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Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers

Partial nucleotide sequences of ORF72 (glycoprotein D, gD), ORF64 (infected cell protein 4, ICP4) and ORF30 (DNA polymerase) genes were compared with corresponding sequences of EHV-1 reference strains to characterize the molecular variability of Brazilian strains. Virus isolation assays were applied...

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Autores principales: Mori, Enio, Lara, Maria do Carmo C.S.H., Cunha, Elenice M.S., Villalobos, Eliana M.C., Mori, Claudia M.C., Soares, Rodrigo M., Brandão, Paulo E., Fernandes, Wilson R., Richtzenhain, Leonardo J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Microbiologia 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507552/
https://www.ncbi.nlm.nih.gov/pubmed/26273275
http://dx.doi.org/10.1590/S1517-838246220140096
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author Mori, Enio
Lara, Maria do Carmo C.S.H.
Cunha, Elenice M.S.
Villalobos, Eliana M.C.
Mori, Claudia M.C.
Soares, Rodrigo M.
Brandão, Paulo E.
Fernandes, Wilson R.
Richtzenhain, Leonardo J.
author_facet Mori, Enio
Lara, Maria do Carmo C.S.H.
Cunha, Elenice M.S.
Villalobos, Eliana M.C.
Mori, Claudia M.C.
Soares, Rodrigo M.
Brandão, Paulo E.
Fernandes, Wilson R.
Richtzenhain, Leonardo J.
author_sort Mori, Enio
collection PubMed
description Partial nucleotide sequences of ORF72 (glycoprotein D, gD), ORF64 (infected cell protein 4, ICP4) and ORF30 (DNA polymerase) genes were compared with corresponding sequences of EHV-1 reference strains to characterize the molecular variability of Brazilian strains. Virus isolation assays were applied to 74 samples including visceral tissue, total blood, cerebrospinal fluid (CSF) and nasal swabs of specimens from a total of 64 animals. Only one CSF sample (Iso07/05 strain) was positive by virus isolation in cell culture. EHV-1 Iso07/05 neurologic strain and two abortion visceral tissues samples (Iso11/06 and Iso33/06) were PCR-positive for ORF33 (glycoprotein B, gB) gene of EHV-1. A sequence analysis of the ORF72, ORF64 and ORF30 genes from three EHV-1 archival strains (A3/97, A4/72, A9/92) and three clinical samples (Iso07/05, Iso11/06 and Iso33/06) suggested that among Brazilian EHV-1 strains, the amplified region of the gD gene sequence is highly conserved. Additionally, the analysis of ICP4 gene showed high nucleotide and amino acid identities when compared with genotype P strains, suggesting that the EHV-1 Brazilian strains belonged to the same group. All the EHV-1 Brazilian strains were classified as non-neuropathogenic variants (N752) based on the ORF30 analysis. These findings indicate a high conservation of the gD-, ICP4- and ORF30-encoding sequences. Different pathotypes of the EHV-1 strain might share identical genes with no specific markers, and tissue tropism is not completely dependent on the gD envelope, immediate-early ICP4 and DNA polymerase proteins.
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spelling pubmed-45075522015-08-13 Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers Mori, Enio Lara, Maria do Carmo C.S.H. Cunha, Elenice M.S. Villalobos, Eliana M.C. Mori, Claudia M.C. Soares, Rodrigo M. Brandão, Paulo E. Fernandes, Wilson R. Richtzenhain, Leonardo J. Braz J Microbiol Veterinary Microbiology Partial nucleotide sequences of ORF72 (glycoprotein D, gD), ORF64 (infected cell protein 4, ICP4) and ORF30 (DNA polymerase) genes were compared with corresponding sequences of EHV-1 reference strains to characterize the molecular variability of Brazilian strains. Virus isolation assays were applied to 74 samples including visceral tissue, total blood, cerebrospinal fluid (CSF) and nasal swabs of specimens from a total of 64 animals. Only one CSF sample (Iso07/05 strain) was positive by virus isolation in cell culture. EHV-1 Iso07/05 neurologic strain and two abortion visceral tissues samples (Iso11/06 and Iso33/06) were PCR-positive for ORF33 (glycoprotein B, gB) gene of EHV-1. A sequence analysis of the ORF72, ORF64 and ORF30 genes from three EHV-1 archival strains (A3/97, A4/72, A9/92) and three clinical samples (Iso07/05, Iso11/06 and Iso33/06) suggested that among Brazilian EHV-1 strains, the amplified region of the gD gene sequence is highly conserved. Additionally, the analysis of ICP4 gene showed high nucleotide and amino acid identities when compared with genotype P strains, suggesting that the EHV-1 Brazilian strains belonged to the same group. All the EHV-1 Brazilian strains were classified as non-neuropathogenic variants (N752) based on the ORF30 analysis. These findings indicate a high conservation of the gD-, ICP4- and ORF30-encoding sequences. Different pathotypes of the EHV-1 strain might share identical genes with no specific markers, and tissue tropism is not completely dependent on the gD envelope, immediate-early ICP4 and DNA polymerase proteins. Sociedade Brasileira de Microbiologia 2015-06-01 /pmc/articles/PMC4507552/ /pubmed/26273275 http://dx.doi.org/10.1590/S1517-838246220140096 Text en Copyright © 2015, Sociedade Brasileira de Microbiologia http://creativecommons.org/licenses/by/3.0/ All the content of the journal, except where otherwise noted, is licensed under a Creative Commons License CC BY-NC.
spellingShingle Veterinary Microbiology
Mori, Enio
Lara, Maria do Carmo C.S.H.
Cunha, Elenice M.S.
Villalobos, Eliana M.C.
Mori, Claudia M.C.
Soares, Rodrigo M.
Brandão, Paulo E.
Fernandes, Wilson R.
Richtzenhain, Leonardo J.
Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers
title Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers
title_full Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers
title_fullStr Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers
title_full_unstemmed Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers
title_short Molecular characterization of Brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers
title_sort molecular characterization of brazilian equid herpesvirus type 1 strains based on neuropathogenicity markers
topic Veterinary Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507552/
https://www.ncbi.nlm.nih.gov/pubmed/26273275
http://dx.doi.org/10.1590/S1517-838246220140096
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