Antimicrobial-resistant and ESBL-producing Escherichia coli in different ecological niches in Bangladesh

INTRODUCTION: The rapid and wide-scale environmental spread of multidrug-resistant bacteria in different ecosystems has become a serious issue in recent years. OBJECTIVES: To investigate the epidemiology of antimicrobial resistance and extended spectrum beta-lactamase (ESBL) in Bangladeshi wild bird...

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Autores principales: Rashid, Mahmudur, Rakib, Mufti Mahmud, Hasan, Badrul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Co-Action Publishing 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507753/
https://www.ncbi.nlm.nih.gov/pubmed/26193990
http://dx.doi.org/10.3402/iee.v5.26712
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author Rashid, Mahmudur
Rakib, Mufti Mahmud
Hasan, Badrul
author_facet Rashid, Mahmudur
Rakib, Mufti Mahmud
Hasan, Badrul
author_sort Rashid, Mahmudur
collection PubMed
description INTRODUCTION: The rapid and wide-scale environmental spread of multidrug-resistant bacteria in different ecosystems has become a serious issue in recent years. OBJECTIVES: To investigate the epidemiology of antimicrobial resistance and extended spectrum beta-lactamase (ESBL) in Bangladeshi wild birds and aquatic environments, samples were taken from Open Bill Stork (Anastomus oscitans) (OBS) and the nearby water sources. METHODS: Water and fresh fecal samples were collected from several locations. All samples were processed and cultured for Escherichia coli and tested for antibiotic susceptibility against commonly used antibiotics. ESBL producers were characterized at genotypic level using polymerase chain reaction (PCR), sequencing, multilocus sequence typing, and rep-PCR. RESULTS AND DISCUSSION: A total of 76 E. coli isolates from the 170 OBS and 8 E. coli isolates from three river sources were isolated. In total, 29% of E. coli isolated from OBS and all of the E. coli isolated from water sources were resistant to at least one of the tested antimicrobials. Resistant phenotypes were observed with all antimicrobials except tigecycline, gentamicin, imipenem, and chloramphenicol. Multidrug resistance was observed in 2.6% of OBS and 37.5% of the water isolates. Also, 1.2% of the ESBL-producing E. coli were isolated from OBS, whereas 50% of the E. coli isolated from water sources were ESBL producers possessing the CTX-M-15 gene. The most concerning aspect of our findings was the presence of human-associated E. coli sequence types in the water samples, for example, ST156-complex156, ST10-complex10 and ST46. CONCLUSION: This study reports the presence of multidrug-resistant ESBL-producing E. coli in OBSs and nearby aquatic sources in Bangladesh.
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spelling pubmed-45077532015-08-10 Antimicrobial-resistant and ESBL-producing Escherichia coli in different ecological niches in Bangladesh Rashid, Mahmudur Rakib, Mufti Mahmud Hasan, Badrul Infect Ecol Epidemiol Original Research Article INTRODUCTION: The rapid and wide-scale environmental spread of multidrug-resistant bacteria in different ecosystems has become a serious issue in recent years. OBJECTIVES: To investigate the epidemiology of antimicrobial resistance and extended spectrum beta-lactamase (ESBL) in Bangladeshi wild birds and aquatic environments, samples were taken from Open Bill Stork (Anastomus oscitans) (OBS) and the nearby water sources. METHODS: Water and fresh fecal samples were collected from several locations. All samples were processed and cultured for Escherichia coli and tested for antibiotic susceptibility against commonly used antibiotics. ESBL producers were characterized at genotypic level using polymerase chain reaction (PCR), sequencing, multilocus sequence typing, and rep-PCR. RESULTS AND DISCUSSION: A total of 76 E. coli isolates from the 170 OBS and 8 E. coli isolates from three river sources were isolated. In total, 29% of E. coli isolated from OBS and all of the E. coli isolated from water sources were resistant to at least one of the tested antimicrobials. Resistant phenotypes were observed with all antimicrobials except tigecycline, gentamicin, imipenem, and chloramphenicol. Multidrug resistance was observed in 2.6% of OBS and 37.5% of the water isolates. Also, 1.2% of the ESBL-producing E. coli were isolated from OBS, whereas 50% of the E. coli isolated from water sources were ESBL producers possessing the CTX-M-15 gene. The most concerning aspect of our findings was the presence of human-associated E. coli sequence types in the water samples, for example, ST156-complex156, ST10-complex10 and ST46. CONCLUSION: This study reports the presence of multidrug-resistant ESBL-producing E. coli in OBSs and nearby aquatic sources in Bangladesh. Co-Action Publishing 2015-07-17 /pmc/articles/PMC4507753/ /pubmed/26193990 http://dx.doi.org/10.3402/iee.v5.26712 Text en © 2015 Mahmudur Rashid et al. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Article
Rashid, Mahmudur
Rakib, Mufti Mahmud
Hasan, Badrul
Antimicrobial-resistant and ESBL-producing Escherichia coli in different ecological niches in Bangladesh
title Antimicrobial-resistant and ESBL-producing Escherichia coli in different ecological niches in Bangladesh
title_full Antimicrobial-resistant and ESBL-producing Escherichia coli in different ecological niches in Bangladesh
title_fullStr Antimicrobial-resistant and ESBL-producing Escherichia coli in different ecological niches in Bangladesh
title_full_unstemmed Antimicrobial-resistant and ESBL-producing Escherichia coli in different ecological niches in Bangladesh
title_short Antimicrobial-resistant and ESBL-producing Escherichia coli in different ecological niches in Bangladesh
title_sort antimicrobial-resistant and esbl-producing escherichia coli in different ecological niches in bangladesh
topic Original Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507753/
https://www.ncbi.nlm.nih.gov/pubmed/26193990
http://dx.doi.org/10.3402/iee.v5.26712
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