Cargando…
A mex3 homolog is required for differentiation during planarian stem cell lineage development
Neoblasts are adult stem cells (ASCs) in planarians that sustain cell replacement during homeostasis and regeneration of any missing tissue. While numerous studies have examined genes underlying neoblast pluripotency, molecular pathways driving postmitotic fates remain poorly defined. In this study,...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507787/ https://www.ncbi.nlm.nih.gov/pubmed/26114597 http://dx.doi.org/10.7554/eLife.07025 |
_version_ | 1782381850800422912 |
---|---|
author | Zhu, Shu Jun Hallows, Stephanie E Currie, Ko W Xu, ChangJiang Pearson, Bret J |
author_facet | Zhu, Shu Jun Hallows, Stephanie E Currie, Ko W Xu, ChangJiang Pearson, Bret J |
author_sort | Zhu, Shu Jun |
collection | PubMed |
description | Neoblasts are adult stem cells (ASCs) in planarians that sustain cell replacement during homeostasis and regeneration of any missing tissue. While numerous studies have examined genes underlying neoblast pluripotency, molecular pathways driving postmitotic fates remain poorly defined. In this study, we used transcriptional profiling of irradiation-sensitive and irradiation-insensitive cell populations and RNA interference (RNAi) functional screening to uncover markers and regulators of postmitotic progeny. We identified 32 new markers distinguishing two main epithelial progenitor populations and a planarian homolog to the MEX3 RNA-binding protein (Smed-mex3-1) as a key regulator of lineage progression. mex3-1 was required for generating differentiated cells of multiple lineages, while restricting the size of the stem cell compartment. We also demonstrated the utility of using mex3-1(RNAi) animals to identify additional progenitor markers. These results identified mex3-1 as a cell fate regulator, broadly required for differentiation, and suggest that mex3-1 helps to mediate the balance between ASC self-renewal and commitment. DOI: http://dx.doi.org/10.7554/eLife.07025.001 |
format | Online Article Text |
id | pubmed-4507787 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-45077872015-07-21 A mex3 homolog is required for differentiation during planarian stem cell lineage development Zhu, Shu Jun Hallows, Stephanie E Currie, Ko W Xu, ChangJiang Pearson, Bret J eLife Developmental Biology and Stem Cells Neoblasts are adult stem cells (ASCs) in planarians that sustain cell replacement during homeostasis and regeneration of any missing tissue. While numerous studies have examined genes underlying neoblast pluripotency, molecular pathways driving postmitotic fates remain poorly defined. In this study, we used transcriptional profiling of irradiation-sensitive and irradiation-insensitive cell populations and RNA interference (RNAi) functional screening to uncover markers and regulators of postmitotic progeny. We identified 32 new markers distinguishing two main epithelial progenitor populations and a planarian homolog to the MEX3 RNA-binding protein (Smed-mex3-1) as a key regulator of lineage progression. mex3-1 was required for generating differentiated cells of multiple lineages, while restricting the size of the stem cell compartment. We also demonstrated the utility of using mex3-1(RNAi) animals to identify additional progenitor markers. These results identified mex3-1 as a cell fate regulator, broadly required for differentiation, and suggest that mex3-1 helps to mediate the balance between ASC self-renewal and commitment. DOI: http://dx.doi.org/10.7554/eLife.07025.001 eLife Sciences Publications, Ltd 2015-06-26 /pmc/articles/PMC4507787/ /pubmed/26114597 http://dx.doi.org/10.7554/eLife.07025 Text en © 2015, Zhu et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Developmental Biology and Stem Cells Zhu, Shu Jun Hallows, Stephanie E Currie, Ko W Xu, ChangJiang Pearson, Bret J A mex3 homolog is required for differentiation during planarian stem cell lineage development |
title | A mex3 homolog is required for differentiation during planarian stem cell lineage development |
title_full | A mex3 homolog is required for differentiation during planarian stem cell lineage development |
title_fullStr | A mex3 homolog is required for differentiation during planarian stem cell lineage development |
title_full_unstemmed | A mex3 homolog is required for differentiation during planarian stem cell lineage development |
title_short | A mex3 homolog is required for differentiation during planarian stem cell lineage development |
title_sort | mex3 homolog is required for differentiation during planarian stem cell lineage development |
topic | Developmental Biology and Stem Cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507787/ https://www.ncbi.nlm.nih.gov/pubmed/26114597 http://dx.doi.org/10.7554/eLife.07025 |
work_keys_str_mv | AT zhushujun amex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment AT hallowsstephaniee amex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment AT curriekow amex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment AT xuchangjiang amex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment AT pearsonbretj amex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment AT zhushujun mex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment AT hallowsstephaniee mex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment AT curriekow mex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment AT xuchangjiang mex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment AT pearsonbretj mex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment |