Cargando…

A mex3 homolog is required for differentiation during planarian stem cell lineage development

Neoblasts are adult stem cells (ASCs) in planarians that sustain cell replacement during homeostasis and regeneration of any missing tissue. While numerous studies have examined genes underlying neoblast pluripotency, molecular pathways driving postmitotic fates remain poorly defined. In this study,...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhu, Shu Jun, Hallows, Stephanie E, Currie, Ko W, Xu, ChangJiang, Pearson, Bret J
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507787/
https://www.ncbi.nlm.nih.gov/pubmed/26114597
http://dx.doi.org/10.7554/eLife.07025
_version_ 1782381850800422912
author Zhu, Shu Jun
Hallows, Stephanie E
Currie, Ko W
Xu, ChangJiang
Pearson, Bret J
author_facet Zhu, Shu Jun
Hallows, Stephanie E
Currie, Ko W
Xu, ChangJiang
Pearson, Bret J
author_sort Zhu, Shu Jun
collection PubMed
description Neoblasts are adult stem cells (ASCs) in planarians that sustain cell replacement during homeostasis and regeneration of any missing tissue. While numerous studies have examined genes underlying neoblast pluripotency, molecular pathways driving postmitotic fates remain poorly defined. In this study, we used transcriptional profiling of irradiation-sensitive and irradiation-insensitive cell populations and RNA interference (RNAi) functional screening to uncover markers and regulators of postmitotic progeny. We identified 32 new markers distinguishing two main epithelial progenitor populations and a planarian homolog to the MEX3 RNA-binding protein (Smed-mex3-1) as a key regulator of lineage progression. mex3-1 was required for generating differentiated cells of multiple lineages, while restricting the size of the stem cell compartment. We also demonstrated the utility of using mex3-1(RNAi) animals to identify additional progenitor markers. These results identified mex3-1 as a cell fate regulator, broadly required for differentiation, and suggest that mex3-1 helps to mediate the balance between ASC self-renewal and commitment. DOI: http://dx.doi.org/10.7554/eLife.07025.001
format Online
Article
Text
id pubmed-4507787
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher eLife Sciences Publications, Ltd
record_format MEDLINE/PubMed
spelling pubmed-45077872015-07-21 A mex3 homolog is required for differentiation during planarian stem cell lineage development Zhu, Shu Jun Hallows, Stephanie E Currie, Ko W Xu, ChangJiang Pearson, Bret J eLife Developmental Biology and Stem Cells Neoblasts are adult stem cells (ASCs) in planarians that sustain cell replacement during homeostasis and regeneration of any missing tissue. While numerous studies have examined genes underlying neoblast pluripotency, molecular pathways driving postmitotic fates remain poorly defined. In this study, we used transcriptional profiling of irradiation-sensitive and irradiation-insensitive cell populations and RNA interference (RNAi) functional screening to uncover markers and regulators of postmitotic progeny. We identified 32 new markers distinguishing two main epithelial progenitor populations and a planarian homolog to the MEX3 RNA-binding protein (Smed-mex3-1) as a key regulator of lineage progression. mex3-1 was required for generating differentiated cells of multiple lineages, while restricting the size of the stem cell compartment. We also demonstrated the utility of using mex3-1(RNAi) animals to identify additional progenitor markers. These results identified mex3-1 as a cell fate regulator, broadly required for differentiation, and suggest that mex3-1 helps to mediate the balance between ASC self-renewal and commitment. DOI: http://dx.doi.org/10.7554/eLife.07025.001 eLife Sciences Publications, Ltd 2015-06-26 /pmc/articles/PMC4507787/ /pubmed/26114597 http://dx.doi.org/10.7554/eLife.07025 Text en © 2015, Zhu et al http://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Developmental Biology and Stem Cells
Zhu, Shu Jun
Hallows, Stephanie E
Currie, Ko W
Xu, ChangJiang
Pearson, Bret J
A mex3 homolog is required for differentiation during planarian stem cell lineage development
title A mex3 homolog is required for differentiation during planarian stem cell lineage development
title_full A mex3 homolog is required for differentiation during planarian stem cell lineage development
title_fullStr A mex3 homolog is required for differentiation during planarian stem cell lineage development
title_full_unstemmed A mex3 homolog is required for differentiation during planarian stem cell lineage development
title_short A mex3 homolog is required for differentiation during planarian stem cell lineage development
title_sort mex3 homolog is required for differentiation during planarian stem cell lineage development
topic Developmental Biology and Stem Cells
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507787/
https://www.ncbi.nlm.nih.gov/pubmed/26114597
http://dx.doi.org/10.7554/eLife.07025
work_keys_str_mv AT zhushujun amex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment
AT hallowsstephaniee amex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment
AT curriekow amex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment
AT xuchangjiang amex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment
AT pearsonbretj amex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment
AT zhushujun mex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment
AT hallowsstephaniee mex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment
AT curriekow mex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment
AT xuchangjiang mex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment
AT pearsonbretj mex3homologisrequiredfordifferentiationduringplanarianstemcelllineagedevelopment