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Improved Gene Targeting through Cell Cycle Synchronization

Gene targeting is a challenge in organisms where non-homologous end-joining is the predominant form of recombination. We show that cell division cycle synchronization can be applied to significantly increase the rate of homologous recombination during transformation. Using hydroxyurea-mediated cell...

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Detalles Bibliográficos
Autores principales:	Tsakraklides, Vasiliki, Brevnova, Elena, Stephanopoulos, Gregory, Shaw, A. Joe
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507847/ https://www.ncbi.nlm.nih.gov/pubmed/26192309 http://dx.doi.org/10.1371/journal.pone.0133434

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