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Decellularization and Delipidation Protocols of Bovine Bone and Pericardium for Bone Grafting and Guided Bone Regeneration Procedures

The combination of bone grafting materials with guided bone regeneration (GBR) membranes seems to provide promising results to restore bone defects in dental clinical practice. In the first part of this work, a novel protocol for decellularization and delipidation of bovine bone, based on multiple s...

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Autores principales: Gardin, Chiara, Ricci, Sara, Ferroni, Letizia, Guazzo, Riccardo, Sbricoli, Luca, De Benedictis, Giulia, Finotti, Luca, Isola, Maurizio, Bressan, Eriberto, Zavan, Barbara
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507977/
https://www.ncbi.nlm.nih.gov/pubmed/26191793
http://dx.doi.org/10.1371/journal.pone.0132344
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author Gardin, Chiara
Ricci, Sara
Ferroni, Letizia
Guazzo, Riccardo
Sbricoli, Luca
De Benedictis, Giulia
Finotti, Luca
Isola, Maurizio
Bressan, Eriberto
Zavan, Barbara
author_facet Gardin, Chiara
Ricci, Sara
Ferroni, Letizia
Guazzo, Riccardo
Sbricoli, Luca
De Benedictis, Giulia
Finotti, Luca
Isola, Maurizio
Bressan, Eriberto
Zavan, Barbara
author_sort Gardin, Chiara
collection PubMed
description The combination of bone grafting materials with guided bone regeneration (GBR) membranes seems to provide promising results to restore bone defects in dental clinical practice. In the first part of this work, a novel protocol for decellularization and delipidation of bovine bone, based on multiple steps of thermal shock, washes with detergent and dehydration with alcohol, is described. This protocol is more effective in removal of cellular materials, and shows superior biocompatibility compared to other three methods tested in this study. Furthermore, histological and morphological analyses confirm the maintenance of an intact bone extracellular matrix (ECM). In vitro and in vivo experiments evidence osteoinductive and osteoconductive properties of the produced scaffold, respectively. In the second part of this study, two methods of bovine pericardium decellularization are compared. The osmotic shock-based protocol gives better results in terms of removal of cell components, biocompatibility, maintenance of native ECM structure, and host tissue reaction, in respect to the freeze/thaw method. Overall, the results of this study demonstrate the characterization of a novel protocol for the decellularization of bovine bone to be used as bone graft, and the acquisition of a method to produce a pericardium membrane suitable for GBR applications.
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spelling pubmed-45079772015-07-24 Decellularization and Delipidation Protocols of Bovine Bone and Pericardium for Bone Grafting and Guided Bone Regeneration Procedures Gardin, Chiara Ricci, Sara Ferroni, Letizia Guazzo, Riccardo Sbricoli, Luca De Benedictis, Giulia Finotti, Luca Isola, Maurizio Bressan, Eriberto Zavan, Barbara PLoS One Research Article The combination of bone grafting materials with guided bone regeneration (GBR) membranes seems to provide promising results to restore bone defects in dental clinical practice. In the first part of this work, a novel protocol for decellularization and delipidation of bovine bone, based on multiple steps of thermal shock, washes with detergent and dehydration with alcohol, is described. This protocol is more effective in removal of cellular materials, and shows superior biocompatibility compared to other three methods tested in this study. Furthermore, histological and morphological analyses confirm the maintenance of an intact bone extracellular matrix (ECM). In vitro and in vivo experiments evidence osteoinductive and osteoconductive properties of the produced scaffold, respectively. In the second part of this study, two methods of bovine pericardium decellularization are compared. The osmotic shock-based protocol gives better results in terms of removal of cell components, biocompatibility, maintenance of native ECM structure, and host tissue reaction, in respect to the freeze/thaw method. Overall, the results of this study demonstrate the characterization of a novel protocol for the decellularization of bovine bone to be used as bone graft, and the acquisition of a method to produce a pericardium membrane suitable for GBR applications. Public Library of Science 2015-07-20 /pmc/articles/PMC4507977/ /pubmed/26191793 http://dx.doi.org/10.1371/journal.pone.0132344 Text en © 2015 Gardin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gardin, Chiara
Ricci, Sara
Ferroni, Letizia
Guazzo, Riccardo
Sbricoli, Luca
De Benedictis, Giulia
Finotti, Luca
Isola, Maurizio
Bressan, Eriberto
Zavan, Barbara
Decellularization and Delipidation Protocols of Bovine Bone and Pericardium for Bone Grafting and Guided Bone Regeneration Procedures
title Decellularization and Delipidation Protocols of Bovine Bone and Pericardium for Bone Grafting and Guided Bone Regeneration Procedures
title_full Decellularization and Delipidation Protocols of Bovine Bone and Pericardium for Bone Grafting and Guided Bone Regeneration Procedures
title_fullStr Decellularization and Delipidation Protocols of Bovine Bone and Pericardium for Bone Grafting and Guided Bone Regeneration Procedures
title_full_unstemmed Decellularization and Delipidation Protocols of Bovine Bone and Pericardium for Bone Grafting and Guided Bone Regeneration Procedures
title_short Decellularization and Delipidation Protocols of Bovine Bone and Pericardium for Bone Grafting and Guided Bone Regeneration Procedures
title_sort decellularization and delipidation protocols of bovine bone and pericardium for bone grafting and guided bone regeneration procedures
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4507977/
https://www.ncbi.nlm.nih.gov/pubmed/26191793
http://dx.doi.org/10.1371/journal.pone.0132344
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