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Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin

The preparative purification of liriodendrin from Sargentodoxa cuneata using macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most...

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Autores principales: Li, Di-Hua, Wang, Yan, Lv, Yuan-Shan, Liu, Jun-Hong, Yang, Lei, Zhang, Shu-Kun, Zhuo, Yu-Zhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4508389/
https://www.ncbi.nlm.nih.gov/pubmed/26236742
http://dx.doi.org/10.1155/2015/861256
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author Li, Di-Hua
Wang, Yan
Lv, Yuan-Shan
Liu, Jun-Hong
Yang, Lei
Zhang, Shu-Kun
Zhuo, Yu-Zhen
author_facet Li, Di-Hua
Wang, Yan
Lv, Yuan-Shan
Liu, Jun-Hong
Yang, Lei
Zhang, Shu-Kun
Zhuo, Yu-Zhen
author_sort Li, Di-Hua
collection PubMed
description The preparative purification of liriodendrin from Sargentodoxa cuneata using macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most suitable medium for liriodendrin purification. The adsorption of liriodendrin on X-5 resin fitted well with the pseudo-second-order kinetic model and Langmuir isotherm model. Dynamic adsorption/desorption tests were performed using a glass column packed with X-5 resin to optimize the separation process of liriodendrin. After one treatment with X-5 resin, the content of liriodendrin in the product was increased 48.73-fold, from 0.85% to 41.42%, with a recovery yield of 88.9%. 97.48% liriodendrin was obtained by further crystallization and determined by HPLC. The purified product possessed strong antioxidant activity. In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy.
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spelling pubmed-45083892015-08-02 Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin Li, Di-Hua Wang, Yan Lv, Yuan-Shan Liu, Jun-Hong Yang, Lei Zhang, Shu-Kun Zhuo, Yu-Zhen Biomed Res Int Research Article The preparative purification of liriodendrin from Sargentodoxa cuneata using macroporous resin combined with crystallization process was evaluated. The properties of adsorption/desorption of liriodendrin on eight macroporous resins were investigated systematically. X-5 resin was selected as the most suitable medium for liriodendrin purification. The adsorption of liriodendrin on X-5 resin fitted well with the pseudo-second-order kinetic model and Langmuir isotherm model. Dynamic adsorption/desorption tests were performed using a glass column packed with X-5 resin to optimize the separation process of liriodendrin. After one treatment with X-5 resin, the content of liriodendrin in the product was increased 48.73-fold, from 0.85% to 41.42%, with a recovery yield of 88.9%. 97.48% liriodendrin was obtained by further crystallization and determined by HPLC. The purified product possessed strong antioxidant activity. In conclusion, purification of liriodendrin might expend its further pharmacological researches and further applications in pharmacy. Hindawi Publishing Corporation 2015 2015-07-07 /pmc/articles/PMC4508389/ /pubmed/26236742 http://dx.doi.org/10.1155/2015/861256 Text en Copyright © 2015 Di-Hua Li et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Di-Hua
Wang, Yan
Lv, Yuan-Shan
Liu, Jun-Hong
Yang, Lei
Zhang, Shu-Kun
Zhuo, Yu-Zhen
Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin
title Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin
title_full Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin
title_fullStr Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin
title_full_unstemmed Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin
title_short Preparative Purification of Liriodendrin from Sargentodoxa cuneata by Macroporous Resin
title_sort preparative purification of liriodendrin from sargentodoxa cuneata by macroporous resin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4508389/
https://www.ncbi.nlm.nih.gov/pubmed/26236742
http://dx.doi.org/10.1155/2015/861256
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