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Combining CXCL10 gene therapy and radiotherapy improved therapeutic efficacy in cervical cancer HeLa cell xenograft tumor models

Radiotherapy is an important treatment method for cervical cancer, but the efficacy requires improvement. Therefore, novel methods of treatment are required. Previous data have demonstrated that the CXC chemokine ligand 10 (CXCL10) inhibits angiogenesis, induces apoptosis and causes avoidance of the...

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Autores principales: ZHAO, MING, MA, QIAN, XU, JINHUI, FU, SHAOZHI, CHEN, LANLAN, WANG, BIQIONG, WU, JINGBO, YANG, LINGLIN
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4508984/
https://www.ncbi.nlm.nih.gov/pubmed/26622567
http://dx.doi.org/10.3892/ol.2015.3281
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author ZHAO, MING
MA, QIAN
XU, JINHUI
FU, SHAOZHI
CHEN, LANLAN
WANG, BIQIONG
WU, JINGBO
YANG, LINGLIN
author_facet ZHAO, MING
MA, QIAN
XU, JINHUI
FU, SHAOZHI
CHEN, LANLAN
WANG, BIQIONG
WU, JINGBO
YANG, LINGLIN
author_sort ZHAO, MING
collection PubMed
description Radiotherapy is an important treatment method for cervical cancer, but the efficacy requires improvement. Therefore, novel methods of treatment are required. Previous data have demonstrated that the CXC chemokine ligand 10 (CXCL10) inhibits angiogenesis, induces apoptosis and causes avoidance of the S phase of the cell cycle in cervical cancer cells. The aim of the present study was to evaluate the anti-tumor effect of radiotherapy combined with CXCL10 gene therapy. Mouse models of cervical carcinoma were created by inoculation with HeLa cells, and were treated by combining intravenously administered plasmid-encoding CXCL10, administered 5 times (days 12, 15, 18, 21 and 24 following inoculation), with direct radiation (20 Gy/5 fractions) administered on 5 consecutive days (~day 27 after inoculation). The vessel density and tumor cell proliferation were observed by immunostaining, and apoptosis was determined using a TUNEL assay. The results revealed a significant increase in the inhibition of tumor growth, reduced vessel density, decreased cell proliferation and increased apoptosis in the tumor cells of the combination therapy group. Overall, these findings resulted in the conclusion that CXCL10 gene therapy in combination with radiotherapy is a novel effective therapeutic strategy for cervical cancer.
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spelling pubmed-45089842015-11-30 Combining CXCL10 gene therapy and radiotherapy improved therapeutic efficacy in cervical cancer HeLa cell xenograft tumor models ZHAO, MING MA, QIAN XU, JINHUI FU, SHAOZHI CHEN, LANLAN WANG, BIQIONG WU, JINGBO YANG, LINGLIN Oncol Lett Articles Radiotherapy is an important treatment method for cervical cancer, but the efficacy requires improvement. Therefore, novel methods of treatment are required. Previous data have demonstrated that the CXC chemokine ligand 10 (CXCL10) inhibits angiogenesis, induces apoptosis and causes avoidance of the S phase of the cell cycle in cervical cancer cells. The aim of the present study was to evaluate the anti-tumor effect of radiotherapy combined with CXCL10 gene therapy. Mouse models of cervical carcinoma were created by inoculation with HeLa cells, and were treated by combining intravenously administered plasmid-encoding CXCL10, administered 5 times (days 12, 15, 18, 21 and 24 following inoculation), with direct radiation (20 Gy/5 fractions) administered on 5 consecutive days (~day 27 after inoculation). The vessel density and tumor cell proliferation were observed by immunostaining, and apoptosis was determined using a TUNEL assay. The results revealed a significant increase in the inhibition of tumor growth, reduced vessel density, decreased cell proliferation and increased apoptosis in the tumor cells of the combination therapy group. Overall, these findings resulted in the conclusion that CXCL10 gene therapy in combination with radiotherapy is a novel effective therapeutic strategy for cervical cancer. D.A. Spandidos 2015-08 2015-05-27 /pmc/articles/PMC4508984/ /pubmed/26622567 http://dx.doi.org/10.3892/ol.2015.3281 Text en Copyright: © Zhao et al. This is an open access article distributed under the terms of a Creative Commons Attribution License. http://creativecommons.org/licenses/by/4.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 4.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
ZHAO, MING
MA, QIAN
XU, JINHUI
FU, SHAOZHI
CHEN, LANLAN
WANG, BIQIONG
WU, JINGBO
YANG, LINGLIN
Combining CXCL10 gene therapy and radiotherapy improved therapeutic efficacy in cervical cancer HeLa cell xenograft tumor models
title Combining CXCL10 gene therapy and radiotherapy improved therapeutic efficacy in cervical cancer HeLa cell xenograft tumor models
title_full Combining CXCL10 gene therapy and radiotherapy improved therapeutic efficacy in cervical cancer HeLa cell xenograft tumor models
title_fullStr Combining CXCL10 gene therapy and radiotherapy improved therapeutic efficacy in cervical cancer HeLa cell xenograft tumor models
title_full_unstemmed Combining CXCL10 gene therapy and radiotherapy improved therapeutic efficacy in cervical cancer HeLa cell xenograft tumor models
title_short Combining CXCL10 gene therapy and radiotherapy improved therapeutic efficacy in cervical cancer HeLa cell xenograft tumor models
title_sort combining cxcl10 gene therapy and radiotherapy improved therapeutic efficacy in cervical cancer hela cell xenograft tumor models
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4508984/
https://www.ncbi.nlm.nih.gov/pubmed/26622567
http://dx.doi.org/10.3892/ol.2015.3281
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