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Antimicrobial Activity of Calcium Hydroxide and Betamethasone on Enterococcus faecalis; An in vitro Assessment
INTRODUCTION: Calcium hydroxide (CH) is one of the most common intracanal medications. Corticosteroids (CS) are used in endodontics because of their anti-inflammatory activity. This study aimed to evaluate the antimicrobial effect of CH+betamethasone and CH+saline against Enterococcus faecalis (E. f...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Iranian Center for Endodontic Research
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4509127/ https://www.ncbi.nlm.nih.gov/pubmed/26213541 http://dx.doi.org/10.7508/iej.2015.03.008 |
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author | Tabrizizadeh, Mahdi Rasti, Mojtaba Ayatollahi, Fatemeh Mossadegh, Mohammad Hossein Zandi, Hengameh Dehghan, Farzad Mousavi, Zohreh |
author_facet | Tabrizizadeh, Mahdi Rasti, Mojtaba Ayatollahi, Fatemeh Mossadegh, Mohammad Hossein Zandi, Hengameh Dehghan, Farzad Mousavi, Zohreh |
author_sort | Tabrizizadeh, Mahdi |
collection | PubMed |
description | INTRODUCTION: Calcium hydroxide (CH) is one of the most common intracanal medications. Corticosteroids (CS) are used in endodontics because of their anti-inflammatory activity. This study aimed to evaluate the antimicrobial effect of CH+betamethasone and CH+saline against Enterococcus faecalis (E. faecalis) using agar diffusion test and measuring the microbial zone of inhibition (ZOI). METHODS AND MATERIALS: Four plates containing Mueller-Hinton broth and E. faecalis culture media, were prepared. In each plate, 5 holes (5×3 mm) were created and a creamy mixture of CH+betamethasone was inserted into the holes (10 holes for each material). Two holes with ampicillin disks and two empty holes were used as negative and positive controls, respectively. Plates were incubated for 24 h and then the diameter of microbial ZOI was measured. The pH of each mixture was measured by pH meter. Data were analyzed using the Mann-Whitney U test. RESULTS: The mean diameter of ZOI for CH+betamethasone and CH+saline was 3.4 and 3 mm, respectively. The difference was not significant (P=0.143). The pH was 12.5 for CH+saline and 12.3 CH+betamethasone, respectively. CONCLUSION: The mixture of CH+betamethasone had good antimicrobial effects against E. faecalis. Further studies are needed to confirm the value of this mixture in clinical settings. |
format | Online Article Text |
id | pubmed-4509127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Iranian Center for Endodontic Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-45091272015-07-24 Antimicrobial Activity of Calcium Hydroxide and Betamethasone on Enterococcus faecalis; An in vitro Assessment Tabrizizadeh, Mahdi Rasti, Mojtaba Ayatollahi, Fatemeh Mossadegh, Mohammad Hossein Zandi, Hengameh Dehghan, Farzad Mousavi, Zohreh Iran Endod J Original Article INTRODUCTION: Calcium hydroxide (CH) is one of the most common intracanal medications. Corticosteroids (CS) are used in endodontics because of their anti-inflammatory activity. This study aimed to evaluate the antimicrobial effect of CH+betamethasone and CH+saline against Enterococcus faecalis (E. faecalis) using agar diffusion test and measuring the microbial zone of inhibition (ZOI). METHODS AND MATERIALS: Four plates containing Mueller-Hinton broth and E. faecalis culture media, were prepared. In each plate, 5 holes (5×3 mm) were created and a creamy mixture of CH+betamethasone was inserted into the holes (10 holes for each material). Two holes with ampicillin disks and two empty holes were used as negative and positive controls, respectively. Plates were incubated for 24 h and then the diameter of microbial ZOI was measured. The pH of each mixture was measured by pH meter. Data were analyzed using the Mann-Whitney U test. RESULTS: The mean diameter of ZOI for CH+betamethasone and CH+saline was 3.4 and 3 mm, respectively. The difference was not significant (P=0.143). The pH was 12.5 for CH+saline and 12.3 CH+betamethasone, respectively. CONCLUSION: The mixture of CH+betamethasone had good antimicrobial effects against E. faecalis. Further studies are needed to confirm the value of this mixture in clinical settings. Iranian Center for Endodontic Research 2015 2015-07-01 /pmc/articles/PMC4509127/ /pubmed/26213541 http://dx.doi.org/10.7508/iej.2015.03.008 Text en © 2015, Iranian Center for Endodontic Research This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Tabrizizadeh, Mahdi Rasti, Mojtaba Ayatollahi, Fatemeh Mossadegh, Mohammad Hossein Zandi, Hengameh Dehghan, Farzad Mousavi, Zohreh Antimicrobial Activity of Calcium Hydroxide and Betamethasone on Enterococcus faecalis; An in vitro Assessment |
title | Antimicrobial Activity of Calcium Hydroxide and Betamethasone on Enterococcus faecalis; An in vitro Assessment |
title_full | Antimicrobial Activity of Calcium Hydroxide and Betamethasone on Enterococcus faecalis; An in vitro Assessment |
title_fullStr | Antimicrobial Activity of Calcium Hydroxide and Betamethasone on Enterococcus faecalis; An in vitro Assessment |
title_full_unstemmed | Antimicrobial Activity of Calcium Hydroxide and Betamethasone on Enterococcus faecalis; An in vitro Assessment |
title_short | Antimicrobial Activity of Calcium Hydroxide and Betamethasone on Enterococcus faecalis; An in vitro Assessment |
title_sort | antimicrobial activity of calcium hydroxide and betamethasone on enterococcus faecalis; an in vitro assessment |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4509127/ https://www.ncbi.nlm.nih.gov/pubmed/26213541 http://dx.doi.org/10.7508/iej.2015.03.008 |
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