Cargando…
Increased efficiency of evolved group I intron spliceozymes by decreased side product formation
The group I intron ribozyme from Tetrahymena was recently reengineered into a trans-splicing variant that is able to remove 100-nt introns from pre-mRNA, analogous to the spliceosome. These spliceozymes were improved in this study by 10 rounds of evolution in Escherichia coli cells. One clone with i...
Autores principales: | Amini, Zhaleh N., Müller, Ulrich F. |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory Press
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4509937/ https://www.ncbi.nlm.nih.gov/pubmed/26106216 http://dx.doi.org/10.1261/rna.051888.115 |
Ejemplares similares
-
Spliceozymes: Ribozymes that Remove Introns from Pre-mRNAs in Trans
por: Amini, Zhaleh N., et al.
Publicado: (2014) -
Trans-splicing with the group I intron ribozyme from Azoarcus
por: Dolan, Gregory F., et al.
Publicado: (2014) -
Design and Experimental Evolution of trans-Splicing Group I Intron Ribozymes
por: Müller, Ulrich F.
Publicado: (2017) -
Group I introns are widespread in archaea
por: Nawrocki, Eric P, et al.
Publicado: (2018) -
GISSD: Group I Intron Sequence and Structure Database
por: Zhou, Yu, et al.
Publicado: (2008)