pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes

Amphoteric azo dyes were used for internal control of pI values in Comparative two-dimensional Fluorescence Gel Electrophoresis (CoFGE) [1]. The 2D-gel images of separated Escherichia coli proteins as well as those of colored amphoteric dyes separated by isoelectric focussing are presented. The latt...

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Detalles Bibliográficos
Autores principales: Hanneken, Marina, Šlais, Karel, König, Simone
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510154/
https://www.ncbi.nlm.nih.gov/pubmed/26217748
http://dx.doi.org/10.1016/j.dib.2015.03.007
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author Hanneken, Marina
Šlais, Karel
König, Simone
author_facet Hanneken, Marina
Šlais, Karel
König, Simone
author_sort Hanneken, Marina
collection PubMed
description Amphoteric azo dyes were used for internal control of pI values in Comparative two-dimensional Fluorescence Gel Electrophoresis (CoFGE) [1]. The 2D-gel images of separated Escherichia coli proteins as well as those of colored amphoteric dyes separated by isoelectric focussing are presented. The latter were used to correct for variation in the first electrophoretic dimension and further improve protein coordinate assignment in 2D-gel electrophoresis. Data tables are supplied to demonstrate pI-value calibration and the effect on the assignment of protein spot coordinates.
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spelling pubmed-45101542015-07-27 pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes Hanneken, Marina Šlais, Karel König, Simone Data Brief Data Article Amphoteric azo dyes were used for internal control of pI values in Comparative two-dimensional Fluorescence Gel Electrophoresis (CoFGE) [1]. The 2D-gel images of separated Escherichia coli proteins as well as those of colored amphoteric dyes separated by isoelectric focussing are presented. The latter were used to correct for variation in the first electrophoretic dimension and further improve protein coordinate assignment in 2D-gel electrophoresis. Data tables are supplied to demonstrate pI-value calibration and the effect on the assignment of protein spot coordinates. Elsevier 2015-04-01 /pmc/articles/PMC4510154/ /pubmed/26217748 http://dx.doi.org/10.1016/j.dib.2015.03.007 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Hanneken, Marina
Šlais, Karel
König, Simone
pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes
title pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes
title_full pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes
title_fullStr pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes
title_full_unstemmed pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes
title_short pI-Control in Comparative Fluorescence Gel Electrophoresis (CoFGE) using amphoteric azo dyes
title_sort pi-control in comparative fluorescence gel electrophoresis (cofge) using amphoteric azo dyes
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510154/
https://www.ncbi.nlm.nih.gov/pubmed/26217748
http://dx.doi.org/10.1016/j.dib.2015.03.007
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