Cargando…

Elucidating the Ramularia eucalypti species complex

The genus Ramularia includes numerous phytopathogenic species, several of which are economically important. Ramularia eucalypti is currently the only species of this genus known to infect Eucalyptus by causing severe leaf-spotting symptoms on this host. However, several isolates identified as R. euc...

Descripción completa

Detalles Bibliográficos
Autores principales: Videira, S.I.R, Groenewald, J.Z., Kolecka, A., van Haren, L., Boekhout, T., Crous, P.W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510271/
https://www.ncbi.nlm.nih.gov/pubmed/26240445
http://dx.doi.org/10.3767/003158515X685670
_version_ 1782382136781701120
author Videira, S.I.R
Groenewald, J.Z.
Kolecka, A.
van Haren, L.
Boekhout, T.
Crous, P.W.
author_facet Videira, S.I.R
Groenewald, J.Z.
Kolecka, A.
van Haren, L.
Boekhout, T.
Crous, P.W.
author_sort Videira, S.I.R
collection PubMed
description The genus Ramularia includes numerous phytopathogenic species, several of which are economically important. Ramularia eucalypti is currently the only species of this genus known to infect Eucalyptus by causing severe leaf-spotting symptoms on this host. However, several isolates identified as R. eucalypti based on morphology and on nrDNA sequence data of the ITS region have recently been isolated from other plant hosts, from environmental samples and also from human clinical specimens. Identification of closely related species based on morphology is often difficult and the ITS region has previously been shown to be unreliable for species level identification in several genera. In this study we aimed to resolve this species-complex by applying a polyphasic approach involving morphology, multi-gene phylogeny and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Six partial genes (ITS, ACT, TEF1-α, HIS3, GAPDH and RPB2) were amplified and sequenced for a total of 44 isolates representing R. eucalypti s.lat. and closely related species. A multi-gene Bayesian phylogenetic analysis and parsimony analysis were performed, and both the resulting trees showed significant support for separation of seven species in R. eucalypti s.lat., including two previously described (R. eucalypti and R. miae), four novel species here described (R. haroldporteri, R. glennii, R. mali and R. plurivora) and one undescribed Ramularia species (sterile). Additionally, Mycosphaerella nyssicola is newly combined in Ramularia as R. nyssicola. Main mass spectra (MSPs) of several R. eucalypti s.lat. strains were generated using MALDI-TOF MS and were compared through a Principal Component Analysis (PCA) dendogram. The PCA dendrogram supported three clades containing R. plurivora, R. glenni/R. mali and R. eucalypti/R. miae. Although the dendrogram separation of species differed from the phylogenetic analysis, the clinically relevant strains were successfully identified by MALDI-TOF MS.
format Online
Article
Text
id pubmed-4510271
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
record_format MEDLINE/PubMed
spelling pubmed-45102712015-08-03 Elucidating the Ramularia eucalypti species complex Videira, S.I.R Groenewald, J.Z. Kolecka, A. van Haren, L. Boekhout, T. Crous, P.W. Persoonia Research Article The genus Ramularia includes numerous phytopathogenic species, several of which are economically important. Ramularia eucalypti is currently the only species of this genus known to infect Eucalyptus by causing severe leaf-spotting symptoms on this host. However, several isolates identified as R. eucalypti based on morphology and on nrDNA sequence data of the ITS region have recently been isolated from other plant hosts, from environmental samples and also from human clinical specimens. Identification of closely related species based on morphology is often difficult and the ITS region has previously been shown to be unreliable for species level identification in several genera. In this study we aimed to resolve this species-complex by applying a polyphasic approach involving morphology, multi-gene phylogeny and matrix assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). Six partial genes (ITS, ACT, TEF1-α, HIS3, GAPDH and RPB2) were amplified and sequenced for a total of 44 isolates representing R. eucalypti s.lat. and closely related species. A multi-gene Bayesian phylogenetic analysis and parsimony analysis were performed, and both the resulting trees showed significant support for separation of seven species in R. eucalypti s.lat., including two previously described (R. eucalypti and R. miae), four novel species here described (R. haroldporteri, R. glennii, R. mali and R. plurivora) and one undescribed Ramularia species (sterile). Additionally, Mycosphaerella nyssicola is newly combined in Ramularia as R. nyssicola. Main mass spectra (MSPs) of several R. eucalypti s.lat. strains were generated using MALDI-TOF MS and were compared through a Principal Component Analysis (PCA) dendogram. The PCA dendrogram supported three clades containing R. plurivora, R. glenni/R. mali and R. eucalypti/R. miae. Although the dendrogram separation of species differed from the phylogenetic analysis, the clinically relevant strains were successfully identified by MALDI-TOF MS. Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures 2014-11-20 2015-06 /pmc/articles/PMC4510271/ /pubmed/26240445 http://dx.doi.org/10.3767/003158515X685670 Text en © 2014-2015 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode You are free to share - to copy, distribute and transmit the work, under the following conditions: Attribution: You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). Non-commercial: You may not use this work for commercial purposes. No derivative works: You may not alter, transform, or build upon this work. For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode. Any of the above conditions can be waived if you get permission from the copyright holder. Nothing in this license impairs or restricts the author’s moral rights.
spellingShingle Research Article
Videira, S.I.R
Groenewald, J.Z.
Kolecka, A.
van Haren, L.
Boekhout, T.
Crous, P.W.
Elucidating the Ramularia eucalypti species complex
title Elucidating the Ramularia eucalypti species complex
title_full Elucidating the Ramularia eucalypti species complex
title_fullStr Elucidating the Ramularia eucalypti species complex
title_full_unstemmed Elucidating the Ramularia eucalypti species complex
title_short Elucidating the Ramularia eucalypti species complex
title_sort elucidating the ramularia eucalypti species complex
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510271/
https://www.ncbi.nlm.nih.gov/pubmed/26240445
http://dx.doi.org/10.3767/003158515X685670
work_keys_str_mv AT videirasir elucidatingtheramulariaeucalyptispeciescomplex
AT groenewaldjz elucidatingtheramulariaeucalyptispeciescomplex
AT koleckaa elucidatingtheramulariaeucalyptispeciescomplex
AT vanharenl elucidatingtheramulariaeucalyptispeciescomplex
AT boekhoutt elucidatingtheramulariaeucalyptispeciescomplex
AT crouspw elucidatingtheramulariaeucalyptispeciescomplex