Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity

Obesity is associated with impaired mitochondrial function. This study compares mitochondrial protein expression in omental fat in obese and non-obese humans. Omental adipose tissue was obtained by surgical biopsy, adipocytes were purified and mitochondria isolated. Using anion-exchange chromatograp...

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Detalles Bibliográficos
Autores principales: Lindinger, Peter W., Christe, Martine, Eberle, Alex N., Kern, Beatrice, Peterli, Ralph, Peters, Thomas, Jayawardene, Kamburapola J.I., Fearnley, Ian M., Walker, John E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510373/
https://www.ncbi.nlm.nih.gov/pubmed/26217759
http://dx.doi.org/10.1016/j.dib.2015.04.007
Descripción
Sumario:Obesity is associated with impaired mitochondrial function. This study compares mitochondrial protein expression in omental fat in obese and non-obese humans. Omental adipose tissue was obtained by surgical biopsy, adipocytes were purified and mitochondria isolated. Using anion-exchange chromatography, SDS-PAGE and mass-spectrometry, 128 proteins with potentially different abundances in patient groups were identified, 62 of the 128 proteins are mainly localized in the mitochondria. Further quantification of 12 of these 62 proteins by immune dot blot analysis revealed four proteins citrate synthase, HADHA, LETM1 and mitofilin being inversely associated with BMI, and mitofilin being inversely correlated with gender.

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