Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity

Obesity is associated with impaired mitochondrial function. This study compares mitochondrial protein expression in omental fat in obese and non-obese humans. Omental adipose tissue was obtained by surgical biopsy, adipocytes were purified and mitochondria isolated. Using anion-exchange chromatograp...

Descripción completa

Detalles Bibliográficos
Autores principales: Lindinger, Peter W., Christe, Martine, Eberle, Alex N., Kern, Beatrice, Peterli, Ralph, Peters, Thomas, Jayawardene, Kamburapola J.I., Fearnley, Ian M., Walker, John E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510373/
https://www.ncbi.nlm.nih.gov/pubmed/26217759
http://dx.doi.org/10.1016/j.dib.2015.04.007
_version_ 1782382154150313984
author Lindinger, Peter W.
Christe, Martine
Eberle, Alex N.
Kern, Beatrice
Peterli, Ralph
Peters, Thomas
Jayawardene, Kamburapola J.I.
Fearnley, Ian M.
Walker, John E.
author_facet Lindinger, Peter W.
Christe, Martine
Eberle, Alex N.
Kern, Beatrice
Peterli, Ralph
Peters, Thomas
Jayawardene, Kamburapola J.I.
Fearnley, Ian M.
Walker, John E.
author_sort Lindinger, Peter W.
collection PubMed
description Obesity is associated with impaired mitochondrial function. This study compares mitochondrial protein expression in omental fat in obese and non-obese humans. Omental adipose tissue was obtained by surgical biopsy, adipocytes were purified and mitochondria isolated. Using anion-exchange chromatography, SDS-PAGE and mass-spectrometry, 128 proteins with potentially different abundances in patient groups were identified, 62 of the 128 proteins are mainly localized in the mitochondria. Further quantification of 12 of these 62 proteins by immune dot blot analysis revealed four proteins citrate synthase, HADHA, LETM1 and mitofilin being inversely associated with BMI, and mitofilin being inversely correlated with gender.
format Online
Article
Text
id pubmed-4510373
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-45103732015-07-27 Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity Lindinger, Peter W. Christe, Martine Eberle, Alex N. Kern, Beatrice Peterli, Ralph Peters, Thomas Jayawardene, Kamburapola J.I. Fearnley, Ian M. Walker, John E. Data Brief Data Article Obesity is associated with impaired mitochondrial function. This study compares mitochondrial protein expression in omental fat in obese and non-obese humans. Omental adipose tissue was obtained by surgical biopsy, adipocytes were purified and mitochondria isolated. Using anion-exchange chromatography, SDS-PAGE and mass-spectrometry, 128 proteins with potentially different abundances in patient groups were identified, 62 of the 128 proteins are mainly localized in the mitochondria. Further quantification of 12 of these 62 proteins by immune dot blot analysis revealed four proteins citrate synthase, HADHA, LETM1 and mitofilin being inversely associated with BMI, and mitofilin being inversely correlated with gender. Elsevier 2015-04-23 /pmc/articles/PMC4510373/ /pubmed/26217759 http://dx.doi.org/10.1016/j.dib.2015.04.007 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Lindinger, Peter W.
Christe, Martine
Eberle, Alex N.
Kern, Beatrice
Peterli, Ralph
Peters, Thomas
Jayawardene, Kamburapola J.I.
Fearnley, Ian M.
Walker, John E.
Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity
title Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity
title_full Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity
title_fullStr Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity
title_full_unstemmed Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity
title_short Important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity
title_sort important mitochondrial proteins in human omental adipose tissue show reduced expression in obesity
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510373/
https://www.ncbi.nlm.nih.gov/pubmed/26217759
http://dx.doi.org/10.1016/j.dib.2015.04.007
work_keys_str_mv AT lindingerpeterw importantmitochondrialproteinsinhumanomentaladiposetissueshowreducedexpressioninobesity
AT christemartine importantmitochondrialproteinsinhumanomentaladiposetissueshowreducedexpressioninobesity
AT eberlealexn importantmitochondrialproteinsinhumanomentaladiposetissueshowreducedexpressioninobesity
AT kernbeatrice importantmitochondrialproteinsinhumanomentaladiposetissueshowreducedexpressioninobesity
AT peterliralph importantmitochondrialproteinsinhumanomentaladiposetissueshowreducedexpressioninobesity
AT petersthomas importantmitochondrialproteinsinhumanomentaladiposetissueshowreducedexpressioninobesity
AT jayawardenekamburapolaji importantmitochondrialproteinsinhumanomentaladiposetissueshowreducedexpressioninobesity
AT fearnleyianm importantmitochondrialproteinsinhumanomentaladiposetissueshowreducedexpressioninobesity
AT walkerjohne importantmitochondrialproteinsinhumanomentaladiposetissueshowreducedexpressioninobesity

Ejemplares similares