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The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current

In whole-cell patch clamp recordings from layer 5 neocortical neurons, blockade of voltage gated sodium and calcium channels leaves a cesium current that is outward rectifying. This current was originally identified as a “non-specific cationic current”, and subsequently it was hypothesized that it i...

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Autores principales: Revah, Omer, Libman, Lior, Fleidervish, Ilya A., Gutnick, Michael J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510442/
https://www.ncbi.nlm.nih.gov/pubmed/26197082
http://dx.doi.org/10.1371/journal.pone.0132108
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author Revah, Omer
Libman, Lior
Fleidervish, Ilya A.
Gutnick, Michael J.
author_facet Revah, Omer
Libman, Lior
Fleidervish, Ilya A.
Gutnick, Michael J.
author_sort Revah, Omer
collection PubMed
description In whole-cell patch clamp recordings from layer 5 neocortical neurons, blockade of voltage gated sodium and calcium channels leaves a cesium current that is outward rectifying. This current was originally identified as a “non-specific cationic current”, and subsequently it was hypothesized that it is mediated by TRP channels. In order to test this hypothesis, we used fluorescence imaging of intracellular sodium and calcium indicators, and found no evidence to suggest that it is associated with influx of either of these ions to the cell body or dendrites. Moreover, the current is still prominent in neurons from TRPC1(-/-) and TRPC5(-/-) mice. The effects on the current of various blocking agents, and especially its sensitivity to intracellular tetraethylammonium, suggest that it is not a non-specific cationic current, but rather that it is generated by cesium-permeable delayed rectifier potassium channels.
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spelling pubmed-45104422015-07-24 The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current Revah, Omer Libman, Lior Fleidervish, Ilya A. Gutnick, Michael J. PLoS One Research Article In whole-cell patch clamp recordings from layer 5 neocortical neurons, blockade of voltage gated sodium and calcium channels leaves a cesium current that is outward rectifying. This current was originally identified as a “non-specific cationic current”, and subsequently it was hypothesized that it is mediated by TRP channels. In order to test this hypothesis, we used fluorescence imaging of intracellular sodium and calcium indicators, and found no evidence to suggest that it is associated with influx of either of these ions to the cell body or dendrites. Moreover, the current is still prominent in neurons from TRPC1(-/-) and TRPC5(-/-) mice. The effects on the current of various blocking agents, and especially its sensitivity to intracellular tetraethylammonium, suggest that it is not a non-specific cationic current, but rather that it is generated by cesium-permeable delayed rectifier potassium channels. Public Library of Science 2015-07-21 /pmc/articles/PMC4510442/ /pubmed/26197082 http://dx.doi.org/10.1371/journal.pone.0132108 Text en © 2015 Revah et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Revah, Omer
Libman, Lior
Fleidervish, Ilya A.
Gutnick, Michael J.
The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current
title The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current
title_full The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current
title_fullStr The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current
title_full_unstemmed The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current
title_short The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current
title_sort outwardly rectifying current of layer 5 neocortical neurons that was originally identified as “non-specific cationic” is essentially a potassium current
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510442/
https://www.ncbi.nlm.nih.gov/pubmed/26197082
http://dx.doi.org/10.1371/journal.pone.0132108
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