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The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current
In whole-cell patch clamp recordings from layer 5 neocortical neurons, blockade of voltage gated sodium and calcium channels leaves a cesium current that is outward rectifying. This current was originally identified as a “non-specific cationic current”, and subsequently it was hypothesized that it i...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510442/ https://www.ncbi.nlm.nih.gov/pubmed/26197082 http://dx.doi.org/10.1371/journal.pone.0132108 |
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author | Revah, Omer Libman, Lior Fleidervish, Ilya A. Gutnick, Michael J. |
author_facet | Revah, Omer Libman, Lior Fleidervish, Ilya A. Gutnick, Michael J. |
author_sort | Revah, Omer |
collection | PubMed |
description | In whole-cell patch clamp recordings from layer 5 neocortical neurons, blockade of voltage gated sodium and calcium channels leaves a cesium current that is outward rectifying. This current was originally identified as a “non-specific cationic current”, and subsequently it was hypothesized that it is mediated by TRP channels. In order to test this hypothesis, we used fluorescence imaging of intracellular sodium and calcium indicators, and found no evidence to suggest that it is associated with influx of either of these ions to the cell body or dendrites. Moreover, the current is still prominent in neurons from TRPC1(-/-) and TRPC5(-/-) mice. The effects on the current of various blocking agents, and especially its sensitivity to intracellular tetraethylammonium, suggest that it is not a non-specific cationic current, but rather that it is generated by cesium-permeable delayed rectifier potassium channels. |
format | Online Article Text |
id | pubmed-4510442 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45104422015-07-24 The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current Revah, Omer Libman, Lior Fleidervish, Ilya A. Gutnick, Michael J. PLoS One Research Article In whole-cell patch clamp recordings from layer 5 neocortical neurons, blockade of voltage gated sodium and calcium channels leaves a cesium current that is outward rectifying. This current was originally identified as a “non-specific cationic current”, and subsequently it was hypothesized that it is mediated by TRP channels. In order to test this hypothesis, we used fluorescence imaging of intracellular sodium and calcium indicators, and found no evidence to suggest that it is associated with influx of either of these ions to the cell body or dendrites. Moreover, the current is still prominent in neurons from TRPC1(-/-) and TRPC5(-/-) mice. The effects on the current of various blocking agents, and especially its sensitivity to intracellular tetraethylammonium, suggest that it is not a non-specific cationic current, but rather that it is generated by cesium-permeable delayed rectifier potassium channels. Public Library of Science 2015-07-21 /pmc/articles/PMC4510442/ /pubmed/26197082 http://dx.doi.org/10.1371/journal.pone.0132108 Text en © 2015 Revah et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Revah, Omer Libman, Lior Fleidervish, Ilya A. Gutnick, Michael J. The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current |
title | The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current |
title_full | The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current |
title_fullStr | The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current |
title_full_unstemmed | The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current |
title_short | The Outwardly Rectifying Current of Layer 5 Neocortical Neurons that was Originally Identified as “Non-Specific Cationic” Is Essentially a Potassium Current |
title_sort | outwardly rectifying current of layer 5 neocortical neurons that was originally identified as “non-specific cationic” is essentially a potassium current |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510442/ https://www.ncbi.nlm.nih.gov/pubmed/26197082 http://dx.doi.org/10.1371/journal.pone.0132108 |
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