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Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization

The efficient uptake is important for the xylose utilization by Saccharomyces cerevisiae. A heterogenous transporter Mgt05196p was cloned from Meyerozyma guilliermondii and expressed in Saccharomyces cerevisiae[1]. This data article contains the transport characteristics of Mgt05196p in S. cerevisia...

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Autores principales: Wang, Chengqiang, Bao, Xiaoming, Li, Yanwei, Jiao, Chunlei, Hou, Jin, Zhang, Qingzhu, Zhang, Weixin, Liu, Weifeng, Shen, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510445/
https://www.ncbi.nlm.nih.gov/pubmed/26217774
http://dx.doi.org/10.1016/j.dib.2015.05.005
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author Wang, Chengqiang
Bao, Xiaoming
Li, Yanwei
Jiao, Chunlei
Hou, Jin
Zhang, Qingzhu
Zhang, Weixin
Liu, Weifeng
Shen, Yu
author_facet Wang, Chengqiang
Bao, Xiaoming
Li, Yanwei
Jiao, Chunlei
Hou, Jin
Zhang, Qingzhu
Zhang, Weixin
Liu, Weifeng
Shen, Yu
author_sort Wang, Chengqiang
collection PubMed
description The efficient uptake is important for the xylose utilization by Saccharomyces cerevisiae. A heterogenous transporter Mgt05196p was cloned from Meyerozyma guilliermondii and expressed in Saccharomyces cerevisiae[1]. This data article contains the transport characteristics of Mgt05196p in S. cerevisiae. The fluorescence of fusion protein Mgt05196p-GFP expressing strain was located on the cell surface demonstrated that the heterogenous transporter Mgt05196p was targeted to the plasma membrane of S. cerevisiae. The expressing of Mgt05196p in the hxt null S. cerevisiae endowed the strain with the glucose and d-xylose absorption capacity, as well as expressing the native d-xylose transporter Gal2p. The transmembrane domains of Mgt05196p were predicted and compared with the XylEp, whose crystal structure was revealed. And then, the homologous modeling of Mgt05196p was built basing on the XylEp to find out the crucial amino acid residues for sugars binding and transport.
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spelling pubmed-45104452015-07-27 Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization Wang, Chengqiang Bao, Xiaoming Li, Yanwei Jiao, Chunlei Hou, Jin Zhang, Qingzhu Zhang, Weixin Liu, Weifeng Shen, Yu Data Brief Data Article The efficient uptake is important for the xylose utilization by Saccharomyces cerevisiae. A heterogenous transporter Mgt05196p was cloned from Meyerozyma guilliermondii and expressed in Saccharomyces cerevisiae[1]. This data article contains the transport characteristics of Mgt05196p in S. cerevisiae. The fluorescence of fusion protein Mgt05196p-GFP expressing strain was located on the cell surface demonstrated that the heterogenous transporter Mgt05196p was targeted to the plasma membrane of S. cerevisiae. The expressing of Mgt05196p in the hxt null S. cerevisiae endowed the strain with the glucose and d-xylose absorption capacity, as well as expressing the native d-xylose transporter Gal2p. The transmembrane domains of Mgt05196p were predicted and compared with the XylEp, whose crystal structure was revealed. And then, the homologous modeling of Mgt05196p was built basing on the XylEp to find out the crucial amino acid residues for sugars binding and transport. Elsevier 2015-05-19 /pmc/articles/PMC4510445/ /pubmed/26217774 http://dx.doi.org/10.1016/j.dib.2015.05.005 Text en © 2015 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Data Article
Wang, Chengqiang
Bao, Xiaoming
Li, Yanwei
Jiao, Chunlei
Hou, Jin
Zhang, Qingzhu
Zhang, Weixin
Liu, Weifeng
Shen, Yu
Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization
title Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization
title_full Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization
title_fullStr Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization
title_full_unstemmed Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization
title_short Data set for cloning and characterization of heterologous transporters in Saccharomyces cerevisiae and identification of important amino acids for xylose utilization
title_sort data set for cloning and characterization of heterologous transporters in saccharomyces cerevisiae and identification of important amino acids for xylose utilization
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4510445/
https://www.ncbi.nlm.nih.gov/pubmed/26217774
http://dx.doi.org/10.1016/j.dib.2015.05.005
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