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Pre-administration of turmeric prevents methotrexate-induced liver toxicity and oxidative stress
BACKGROUND: Methotrexate (MTX) is an antimetabolite broadly used in treatment of cancer and autoimmune diseases. MTX-induced hepatotoxicity limits its application. We investigated hepatoprotective effects of turmeric in MTX-induced liver toxicity. METHODS: All experiments were performed on male Wist...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511036/ https://www.ncbi.nlm.nih.gov/pubmed/26199067 http://dx.doi.org/10.1186/s12906-015-0773-6 |
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author | Moghadam, Adel Rezaei Tutunchi, Soheil Namvaran-Abbas-Abad, Ali Yazdi, Mina Bonyadi, Fatemeh Mohajeri, Daryoush Mazani, Mohammad Marzban, Hassan Łos, Marek J. Ghavami, Saeid |
author_facet | Moghadam, Adel Rezaei Tutunchi, Soheil Namvaran-Abbas-Abad, Ali Yazdi, Mina Bonyadi, Fatemeh Mohajeri, Daryoush Mazani, Mohammad Marzban, Hassan Łos, Marek J. Ghavami, Saeid |
author_sort | Moghadam, Adel Rezaei |
collection | PubMed |
description | BACKGROUND: Methotrexate (MTX) is an antimetabolite broadly used in treatment of cancer and autoimmune diseases. MTX-induced hepatotoxicity limits its application. We investigated hepatoprotective effects of turmeric in MTX-induced liver toxicity. METHODS: All experiments were performed on male Wistar albino rats that were randomly divided into six groups. Group one received saline orally for 30 days (control group), groups two and three received turmeric extract (100, 200 mg/kg respectively) orally for 30 days, group four received single dose, of MTX IP at day 30, groups five and six received turmeric extract 100 and 200 mg/kg orally respectively for 30 days and single dose of methoterxate IP (20 mg/kg) at day 30. Four days after MTX injection animals were sacrificed and evaluated. Blood ALT and AST (indicators of hepatocyte injury), ALP and bilirubin (markers of biliary function), albumin (reflect liver synthetic function) as well as the plasma TAS concentration (antioxidant defenses) were determined. The cellular antioxidant defense activities were examined in liver tissue samples using SOD, CAT, and GSH-Px for the oxidative stress, and MDA for lipid peroxidation. In addition, liver damage was evaluated histopathologically. RESULTS: MTX significantly induced liver damage (P < 0.05) and decreased its antioxidant capacity, while turmeric was hepatoprotective. Liver tissue microscopic evaluation showed that MTX treatment induced severe centrilobular and periportal degeneration, hyperemia of portal vein, increased artery inflammatory cells infiltration and necrosis, while all of histopathological changes were attenuated by turmeric (200 mg/kg). CONCLUSION: Turmeric extract can successfully attenuate MTX-hepatotoxicity. The effect is partly mediated through extract’s antinflammatory activity. |
format | Online Article Text |
id | pubmed-4511036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-45110362015-07-23 Pre-administration of turmeric prevents methotrexate-induced liver toxicity and oxidative stress Moghadam, Adel Rezaei Tutunchi, Soheil Namvaran-Abbas-Abad, Ali Yazdi, Mina Bonyadi, Fatemeh Mohajeri, Daryoush Mazani, Mohammad Marzban, Hassan Łos, Marek J. Ghavami, Saeid BMC Complement Altern Med Research Article BACKGROUND: Methotrexate (MTX) is an antimetabolite broadly used in treatment of cancer and autoimmune diseases. MTX-induced hepatotoxicity limits its application. We investigated hepatoprotective effects of turmeric in MTX-induced liver toxicity. METHODS: All experiments were performed on male Wistar albino rats that were randomly divided into six groups. Group one received saline orally for 30 days (control group), groups two and three received turmeric extract (100, 200 mg/kg respectively) orally for 30 days, group four received single dose, of MTX IP at day 30, groups five and six received turmeric extract 100 and 200 mg/kg orally respectively for 30 days and single dose of methoterxate IP (20 mg/kg) at day 30. Four days after MTX injection animals were sacrificed and evaluated. Blood ALT and AST (indicators of hepatocyte injury), ALP and bilirubin (markers of biliary function), albumin (reflect liver synthetic function) as well as the plasma TAS concentration (antioxidant defenses) were determined. The cellular antioxidant defense activities were examined in liver tissue samples using SOD, CAT, and GSH-Px for the oxidative stress, and MDA for lipid peroxidation. In addition, liver damage was evaluated histopathologically. RESULTS: MTX significantly induced liver damage (P < 0.05) and decreased its antioxidant capacity, while turmeric was hepatoprotective. Liver tissue microscopic evaluation showed that MTX treatment induced severe centrilobular and periportal degeneration, hyperemia of portal vein, increased artery inflammatory cells infiltration and necrosis, while all of histopathological changes were attenuated by turmeric (200 mg/kg). CONCLUSION: Turmeric extract can successfully attenuate MTX-hepatotoxicity. The effect is partly mediated through extract’s antinflammatory activity. BioMed Central 2015-07-22 /pmc/articles/PMC4511036/ /pubmed/26199067 http://dx.doi.org/10.1186/s12906-015-0773-6 Text en © Moghadam et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Moghadam, Adel Rezaei Tutunchi, Soheil Namvaran-Abbas-Abad, Ali Yazdi, Mina Bonyadi, Fatemeh Mohajeri, Daryoush Mazani, Mohammad Marzban, Hassan Łos, Marek J. Ghavami, Saeid Pre-administration of turmeric prevents methotrexate-induced liver toxicity and oxidative stress |
title | Pre-administration of turmeric prevents methotrexate-induced liver toxicity and oxidative stress |
title_full | Pre-administration of turmeric prevents methotrexate-induced liver toxicity and oxidative stress |
title_fullStr | Pre-administration of turmeric prevents methotrexate-induced liver toxicity and oxidative stress |
title_full_unstemmed | Pre-administration of turmeric prevents methotrexate-induced liver toxicity and oxidative stress |
title_short | Pre-administration of turmeric prevents methotrexate-induced liver toxicity and oxidative stress |
title_sort | pre-administration of turmeric prevents methotrexate-induced liver toxicity and oxidative stress |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511036/ https://www.ncbi.nlm.nih.gov/pubmed/26199067 http://dx.doi.org/10.1186/s12906-015-0773-6 |
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