Biliary tract external drainage increases the expression levels of heme oxygenase-1 in rat livers

BACKGROUND: Heme oxygenase-1 (HO-1) protects cells by anti-oxidation, maintaining normal microcirculation and anti-inflammatory under stress. This study investigated the effects of biliary tract external drainage (BTED) on the expression levels of HO-1 in rat livers. METHODS: Biliary tract external...

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Autores principales: Wang, Lu, Zhao, Bing, Chen, Ying, Ma, Li, Chen, Er-Zhen, Mao, En-Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511237/
https://www.ncbi.nlm.nih.gov/pubmed/26199001
http://dx.doi.org/10.1186/s40001-015-0152-2
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author Wang, Lu
Zhao, Bing
Chen, Ying
Ma, Li
Chen, Er-Zhen
Mao, En-Qiang
author_facet Wang, Lu
Zhao, Bing
Chen, Ying
Ma, Li
Chen, Er-Zhen
Mao, En-Qiang
author_sort Wang, Lu
collection PubMed
description BACKGROUND: Heme oxygenase-1 (HO-1) protects cells by anti-oxidation, maintaining normal microcirculation and anti-inflammatory under stress. This study investigated the effects of biliary tract external drainage (BTED) on the expression levels of HO-1 in rat livers. METHODS: Biliary tract external drainage was performed by inserting a cannula into the bile duct. Sixty Sprague–Dawley rats were randomized to the following groups: sham 1 h group; BTED 1 h group; bile duct ligation (BDL) 1 h group; sham 6 h group and BTED 6 h group. The expression levels of HO-1 mRNA were analyzed using real-time RT-PCR. The expression levels of HO-1 were analyzed using immunohistochemistry. RESULTS: The expression levels of HO-1 mRNA in the liver of the BTED group increased significantly compared with the sham group 1 and 6 h after surgery (p < 0.05).The expression levels of HO-1 in the BTED group increased significantly compared with the sham group 1 and 6 h after surgery. The expression levels of HO-1 mRNA in the liver in the BDL group decreased significantly compared with the sham group 1 h after surgery (p < 0.05).The expression levels of HO-1 in the BDL group decreased significantly compared with the sham group at this time. CONCLUSION: Biliary tract external drainages increase the expression levels of HO-1 in the liver.
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spelling pubmed-45112372015-07-23 Biliary tract external drainage increases the expression levels of heme oxygenase-1 in rat livers Wang, Lu Zhao, Bing Chen, Ying Ma, Li Chen, Er-Zhen Mao, En-Qiang Eur J Med Res Research BACKGROUND: Heme oxygenase-1 (HO-1) protects cells by anti-oxidation, maintaining normal microcirculation and anti-inflammatory under stress. This study investigated the effects of biliary tract external drainage (BTED) on the expression levels of HO-1 in rat livers. METHODS: Biliary tract external drainage was performed by inserting a cannula into the bile duct. Sixty Sprague–Dawley rats were randomized to the following groups: sham 1 h group; BTED 1 h group; bile duct ligation (BDL) 1 h group; sham 6 h group and BTED 6 h group. The expression levels of HO-1 mRNA were analyzed using real-time RT-PCR. The expression levels of HO-1 were analyzed using immunohistochemistry. RESULTS: The expression levels of HO-1 mRNA in the liver of the BTED group increased significantly compared with the sham group 1 and 6 h after surgery (p < 0.05).The expression levels of HO-1 in the BTED group increased significantly compared with the sham group 1 and 6 h after surgery. The expression levels of HO-1 mRNA in the liver in the BDL group decreased significantly compared with the sham group 1 h after surgery (p < 0.05).The expression levels of HO-1 in the BDL group decreased significantly compared with the sham group at this time. CONCLUSION: Biliary tract external drainages increase the expression levels of HO-1 in the liver. BioMed Central 2015-07-22 /pmc/articles/PMC4511237/ /pubmed/26199001 http://dx.doi.org/10.1186/s40001-015-0152-2 Text en © Wang et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Wang, Lu
Zhao, Bing
Chen, Ying
Ma, Li
Chen, Er-Zhen
Mao, En-Qiang
Biliary tract external drainage increases the expression levels of heme oxygenase-1 in rat livers
title Biliary tract external drainage increases the expression levels of heme oxygenase-1 in rat livers
title_full Biliary tract external drainage increases the expression levels of heme oxygenase-1 in rat livers
title_fullStr Biliary tract external drainage increases the expression levels of heme oxygenase-1 in rat livers
title_full_unstemmed Biliary tract external drainage increases the expression levels of heme oxygenase-1 in rat livers
title_short Biliary tract external drainage increases the expression levels of heme oxygenase-1 in rat livers
title_sort biliary tract external drainage increases the expression levels of heme oxygenase-1 in rat livers
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511237/
https://www.ncbi.nlm.nih.gov/pubmed/26199001
http://dx.doi.org/10.1186/s40001-015-0152-2
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