Cryo-electron Microscopy Specimen Preparation By Means Of a Focused Ion Beam

Here we present a protocol used to prepare cryo-TEM samples of Aspergillus niger spores, but which can easily be adapted for any number of microorganisms or solutions. We make use of a custom built cryo-transfer station and a modified cryo-SEM preparation chamber(2). The spores are taken from a cult...

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Detalles Bibliográficos
Autores principales: Rubino, Stefano, Melin, Petter, Spellward, Paul, Leifer, Klaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2014
Materias:
Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511271/
https://www.ncbi.nlm.nih.gov/pubmed/25146386
http://dx.doi.org/10.3791/51463

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511271/
https://www.ncbi.nlm.nih.gov/pubmed/25146386
http://dx.doi.org/10.3791/51463

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