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Cryo-electron Microscopy Specimen Preparation By Means Of a Focused Ion Beam
Here we present a protocol used to prepare cryo-TEM samples of Aspergillus niger spores, but which can easily be adapted for any number of microorganisms or solutions. We make use of a custom built cryo-transfer station and a modified cryo-SEM preparation chamber(2). The spores are taken from a cult...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MyJove Corporation
2014
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511271/ https://www.ncbi.nlm.nih.gov/pubmed/25146386 http://dx.doi.org/10.3791/51463 |