Cargando…

Effect of inhibition proliferation in human lung adenocarcinoma A549 cells by cytokine-induced killer cells

BACKGROUND: Adenocarcinoma, the most common form of lung cancer, is one of main human malignant tumors. In this paper, we focus on the effect of antitumor activity of cytokine-induced killer (CIK) cells on human lung adenocarcinoma cell line A549. METHODS: CIK cells were obtained by inducing periphe...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Dengrui, Guo, Sumin, Li, Hui, Zhu, Guiyun, Gao, Li, Xin, Xin, Yan, Dandan, Li, Xiuwu, Geng, Shujun, Hou, Hongwei, Yang, Yonghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511324/
https://www.ncbi.nlm.nih.gov/pubmed/26273401
http://dx.doi.org/10.1111/1759-7714.12205
_version_ 1782382313977413632
author Li, Dengrui
Guo, Sumin
Li, Hui
Zhu, Guiyun
Gao, Li
Xin, Xin
Yan, Dandan
Li, Xiuwu
Geng, Shujun
Hou, Hongwei
Yang, Yonghui
author_facet Li, Dengrui
Guo, Sumin
Li, Hui
Zhu, Guiyun
Gao, Li
Xin, Xin
Yan, Dandan
Li, Xiuwu
Geng, Shujun
Hou, Hongwei
Yang, Yonghui
author_sort Li, Dengrui
collection PubMed
description BACKGROUND: Adenocarcinoma, the most common form of lung cancer, is one of main human malignant tumors. In this paper, we focus on the effect of antitumor activity of cytokine-induced killer (CIK) cells on human lung adenocarcinoma cell line A549. METHODS: CIK cells were obtained by inducing peripheral blood mononuclear cells with recombinant human (rh) interferon-gamma, monoclonal anti-CD3 antibody, rh interleukin (IL)-1alpha, and rhIL-2, which were added into the culture. A549 cell viability of CIK cells was determined using MTS assay. Flow cytometry (FCM) experiments were performed to detect cell cycle changes. The expression of P27 in A549 cells treated by CIK cells was evaluated by Western blot. RESULT: The percentage of CD3+CD16+CD56+ T cells in a representative peripheral blood mononucleated cell sample was 33.7 ± 1.3%. CIK cells, in dose and time dependent manners, inhibited the proliferation of A549. FCM demonstrated that A549 cells were accumulated in G2/M and G(0)/G(1) phases when treated with CIK cells. FCM was used to analyze whether A549 cells treated with CIK cells induced apotosis or necrosis at 10:1 or 20:1. Compared to the control group, P27 was prominently upregulated in the CIK treated group. CONCLUSION: We propose that the pharmacological mechanisms of A549 cells inhibited by CIK cells can be estimated to possibly elicit different biological significance, which, in part, can be ascribed to a different mass transport rate in vitro.
format Online
Article
Text
id pubmed-4511324
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher John Wiley & Sons, Ltd
record_format MEDLINE/PubMed
spelling pubmed-45113242015-08-13 Effect of inhibition proliferation in human lung adenocarcinoma A549 cells by cytokine-induced killer cells Li, Dengrui Guo, Sumin Li, Hui Zhu, Guiyun Gao, Li Xin, Xin Yan, Dandan Li, Xiuwu Geng, Shujun Hou, Hongwei Yang, Yonghui Thorac Cancer Original Articles BACKGROUND: Adenocarcinoma, the most common form of lung cancer, is one of main human malignant tumors. In this paper, we focus on the effect of antitumor activity of cytokine-induced killer (CIK) cells on human lung adenocarcinoma cell line A549. METHODS: CIK cells were obtained by inducing peripheral blood mononuclear cells with recombinant human (rh) interferon-gamma, monoclonal anti-CD3 antibody, rh interleukin (IL)-1alpha, and rhIL-2, which were added into the culture. A549 cell viability of CIK cells was determined using MTS assay. Flow cytometry (FCM) experiments were performed to detect cell cycle changes. The expression of P27 in A549 cells treated by CIK cells was evaluated by Western blot. RESULT: The percentage of CD3+CD16+CD56+ T cells in a representative peripheral blood mononucleated cell sample was 33.7 ± 1.3%. CIK cells, in dose and time dependent manners, inhibited the proliferation of A549. FCM demonstrated that A549 cells were accumulated in G2/M and G(0)/G(1) phases when treated with CIK cells. FCM was used to analyze whether A549 cells treated with CIK cells induced apotosis or necrosis at 10:1 or 20:1. Compared to the control group, P27 was prominently upregulated in the CIK treated group. CONCLUSION: We propose that the pharmacological mechanisms of A549 cells inhibited by CIK cells can be estimated to possibly elicit different biological significance, which, in part, can be ascribed to a different mass transport rate in vitro. John Wiley & Sons, Ltd 2015-07 2014-12-29 /pmc/articles/PMC4511324/ /pubmed/26273401 http://dx.doi.org/10.1111/1759-7714.12205 Text en © 2014 The Authors. Thoracic Cancer published by Tianjin Lung Cancer Institute and Wiley Publishing Asia Pty Ltd. http://creativecommons.org/licenses/by-nc/4.0/ This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Original Articles
Li, Dengrui
Guo, Sumin
Li, Hui
Zhu, Guiyun
Gao, Li
Xin, Xin
Yan, Dandan
Li, Xiuwu
Geng, Shujun
Hou, Hongwei
Yang, Yonghui
Effect of inhibition proliferation in human lung adenocarcinoma A549 cells by cytokine-induced killer cells
title Effect of inhibition proliferation in human lung adenocarcinoma A549 cells by cytokine-induced killer cells
title_full Effect of inhibition proliferation in human lung adenocarcinoma A549 cells by cytokine-induced killer cells
title_fullStr Effect of inhibition proliferation in human lung adenocarcinoma A549 cells by cytokine-induced killer cells
title_full_unstemmed Effect of inhibition proliferation in human lung adenocarcinoma A549 cells by cytokine-induced killer cells
title_short Effect of inhibition proliferation in human lung adenocarcinoma A549 cells by cytokine-induced killer cells
title_sort effect of inhibition proliferation in human lung adenocarcinoma a549 cells by cytokine-induced killer cells
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4511324/
https://www.ncbi.nlm.nih.gov/pubmed/26273401
http://dx.doi.org/10.1111/1759-7714.12205
work_keys_str_mv AT lidengrui effectofinhibitionproliferationinhumanlungadenocarcinomaa549cellsbycytokineinducedkillercells
AT guosumin effectofinhibitionproliferationinhumanlungadenocarcinomaa549cellsbycytokineinducedkillercells
AT lihui effectofinhibitionproliferationinhumanlungadenocarcinomaa549cellsbycytokineinducedkillercells
AT zhuguiyun effectofinhibitionproliferationinhumanlungadenocarcinomaa549cellsbycytokineinducedkillercells
AT gaoli effectofinhibitionproliferationinhumanlungadenocarcinomaa549cellsbycytokineinducedkillercells
AT xinxin effectofinhibitionproliferationinhumanlungadenocarcinomaa549cellsbycytokineinducedkillercells
AT yandandan effectofinhibitionproliferationinhumanlungadenocarcinomaa549cellsbycytokineinducedkillercells
AT lixiuwu effectofinhibitionproliferationinhumanlungadenocarcinomaa549cellsbycytokineinducedkillercells
AT gengshujun effectofinhibitionproliferationinhumanlungadenocarcinomaa549cellsbycytokineinducedkillercells
AT houhongwei effectofinhibitionproliferationinhumanlungadenocarcinomaa549cellsbycytokineinducedkillercells
AT yangyonghui effectofinhibitionproliferationinhumanlungadenocarcinomaa549cellsbycytokineinducedkillercells