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Characterization and colonization of endomycorrhizal Rhizoctonia fungi in the medicinal herb Anoectochilus formosanus (Orchidaceae)
The medicinal effects and techniques for cultivating Anoectochilus formosanus are well-documented, but little is known about the mycorrhizal fungi associated with A. formosanus. Rhizoctonia (Thanatephorus) anastomosis group 6 (AG-6) was the most common species isolated from fungal pelotons in native...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4512280/ https://www.ncbi.nlm.nih.gov/pubmed/25575732 http://dx.doi.org/10.1007/s00572-014-0616-1 |
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author | Jiang, Jr-Hau Lee, Yung-I Cubeta, Marc A. Chen, Lung-Chung |
author_facet | Jiang, Jr-Hau Lee, Yung-I Cubeta, Marc A. Chen, Lung-Chung |
author_sort | Jiang, Jr-Hau |
collection | PubMed |
description | The medicinal effects and techniques for cultivating Anoectochilus formosanus are well-documented, but little is known about the mycorrhizal fungi associated with A. formosanus. Rhizoctonia (Thanatephorus) anastomosis group 6 (AG-6) was the most common species isolated from fungal pelotons in native A. formosanus and represented 67 % of the sample. Rhizoctonia (Ceratobasidium) AG-G, P, and R were also isolated and represent the first occurrence in the Orchidaceae. Isolates of AG-6, AG-R, and AG-P in clade I increased seed germination 44–91 % and promoted protocorm growth from phases III to VI compared to asymbiotic treatments and isolates of AG-G in clade II and Tulasnella species in clade III. All isolates in clades I to III formed fungal pelotons in tissue-cultured seedlings of A. formosanus, which exhibited significantly greater growth than nonmycorrhizal seedlings. An analysis of the relative effect of treatment ([Formula: see text] ) showed that the low level of colonization ([Formula: see text] ) by isolates in clade I resulted in a significant increase in seedling growth compared to isolates in clades II (0.63–0.82) and III (0.63–0.75). There was also a negative correlation (r = −0.8801) with fresh plant weight and fungal colonization. Our results suggest that isolates in clade I may represent an important group associated with native populations of A. formosanus and can vary in their ability to establish a symbiotic association with A. formosanus. The results presented here are potentially useful for advancing research on the medicinal properties, production, and conservation of A. formosanus in diverse ecosystems. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00572-014-0616-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4512280 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-45122802015-07-24 Characterization and colonization of endomycorrhizal Rhizoctonia fungi in the medicinal herb Anoectochilus formosanus (Orchidaceae) Jiang, Jr-Hau Lee, Yung-I Cubeta, Marc A. Chen, Lung-Chung Mycorrhiza Original Paper The medicinal effects and techniques for cultivating Anoectochilus formosanus are well-documented, but little is known about the mycorrhizal fungi associated with A. formosanus. Rhizoctonia (Thanatephorus) anastomosis group 6 (AG-6) was the most common species isolated from fungal pelotons in native A. formosanus and represented 67 % of the sample. Rhizoctonia (Ceratobasidium) AG-G, P, and R were also isolated and represent the first occurrence in the Orchidaceae. Isolates of AG-6, AG-R, and AG-P in clade I increased seed germination 44–91 % and promoted protocorm growth from phases III to VI compared to asymbiotic treatments and isolates of AG-G in clade II and Tulasnella species in clade III. All isolates in clades I to III formed fungal pelotons in tissue-cultured seedlings of A. formosanus, which exhibited significantly greater growth than nonmycorrhizal seedlings. An analysis of the relative effect of treatment ([Formula: see text] ) showed that the low level of colonization ([Formula: see text] ) by isolates in clade I resulted in a significant increase in seedling growth compared to isolates in clades II (0.63–0.82) and III (0.63–0.75). There was also a negative correlation (r = −0.8801) with fresh plant weight and fungal colonization. Our results suggest that isolates in clade I may represent an important group associated with native populations of A. formosanus and can vary in their ability to establish a symbiotic association with A. formosanus. The results presented here are potentially useful for advancing research on the medicinal properties, production, and conservation of A. formosanus in diverse ecosystems. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00572-014-0616-1) contains supplementary material, which is available to authorized users. Springer Berlin Heidelberg 2015-01-11 2015 /pmc/articles/PMC4512280/ /pubmed/25575732 http://dx.doi.org/10.1007/s00572-014-0616-1 Text en © The Author(s) 2015 https://creativecommons.org/licenses/by/4.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Original Paper Jiang, Jr-Hau Lee, Yung-I Cubeta, Marc A. Chen, Lung-Chung Characterization and colonization of endomycorrhizal Rhizoctonia fungi in the medicinal herb Anoectochilus formosanus (Orchidaceae) |
title | Characterization and colonization of endomycorrhizal Rhizoctonia fungi in the medicinal herb Anoectochilus formosanus (Orchidaceae) |
title_full | Characterization and colonization of endomycorrhizal Rhizoctonia fungi in the medicinal herb Anoectochilus formosanus (Orchidaceae) |
title_fullStr | Characterization and colonization of endomycorrhizal Rhizoctonia fungi in the medicinal herb Anoectochilus formosanus (Orchidaceae) |
title_full_unstemmed | Characterization and colonization of endomycorrhizal Rhizoctonia fungi in the medicinal herb Anoectochilus formosanus (Orchidaceae) |
title_short | Characterization and colonization of endomycorrhizal Rhizoctonia fungi in the medicinal herb Anoectochilus formosanus (Orchidaceae) |
title_sort | characterization and colonization of endomycorrhizal rhizoctonia fungi in the medicinal herb anoectochilus formosanus (orchidaceae) |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4512280/ https://www.ncbi.nlm.nih.gov/pubmed/25575732 http://dx.doi.org/10.1007/s00572-014-0616-1 |
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