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Tissue Remodelling following Resection of Porcine Liver

Aim. To study genes regulating the extracellular matrix (ECM) and investigate the tissue remodelling following liver resection in porcine. Methods. Four pigs with 60% partial hepatectomy- (PHx-) induced liver regeneration were studied over six weeks. Four pigs underwent sham surgery and another four...

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Autores principales: Nygård, Ingvild Engdal, Mortensen, Kim Erlend, Hedegaard, Jakob, Conley, Lene Nagstrup, Bendixen, Christian, Sveinbjørnsson, Baldur, Revhaug, Arthur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4512564/
https://www.ncbi.nlm.nih.gov/pubmed/26240819
http://dx.doi.org/10.1155/2015/248920
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author Nygård, Ingvild Engdal
Mortensen, Kim Erlend
Hedegaard, Jakob
Conley, Lene Nagstrup
Bendixen, Christian
Sveinbjørnsson, Baldur
Revhaug, Arthur
author_facet Nygård, Ingvild Engdal
Mortensen, Kim Erlend
Hedegaard, Jakob
Conley, Lene Nagstrup
Bendixen, Christian
Sveinbjørnsson, Baldur
Revhaug, Arthur
author_sort Nygård, Ingvild Engdal
collection PubMed
description Aim. To study genes regulating the extracellular matrix (ECM) and investigate the tissue remodelling following liver resection in porcine. Methods. Four pigs with 60% partial hepatectomy- (PHx-) induced liver regeneration were studied over six weeks. Four pigs underwent sham surgery and another four pigs were used as controls of the normal liver growth. Liver biopsies were taken upon laparotomy, after three and six weeks. Gene expression profiles were obtained using porcine-specific oligonucleotide microarrays. Immunohistochemical staining was performed and a proliferative index was assessed. Results. More differentially expressed genes were associated with the regulation of ECM in the resection group compared to the sham and control groups. Secreted protein acidic and rich in cysteine (SPARC) and collagen 1, alpha 2 (COL1A2) were both upregulated in the early phase of liver regeneration, validated by immunopositive cells during the remodelling phase of liver regeneration. A broadened connective tissue was demonstrated by Masson's Trichrome staining, and an immunohistochemical staining against pan-Cytokeratin (pan-CK) demonstrated a distinct pattern of migrating cells, followed by proliferating cell nuclear antigen (PCNA) positive nuclei. Conclusions. The present study demonstrates both a distinct pattern of PCNA positive nuclei and a deposition of ECM proteins in the remodelling phase of liver regeneration.
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spelling pubmed-45125642015-08-03 Tissue Remodelling following Resection of Porcine Liver Nygård, Ingvild Engdal Mortensen, Kim Erlend Hedegaard, Jakob Conley, Lene Nagstrup Bendixen, Christian Sveinbjørnsson, Baldur Revhaug, Arthur Biomed Res Int Research Article Aim. To study genes regulating the extracellular matrix (ECM) and investigate the tissue remodelling following liver resection in porcine. Methods. Four pigs with 60% partial hepatectomy- (PHx-) induced liver regeneration were studied over six weeks. Four pigs underwent sham surgery and another four pigs were used as controls of the normal liver growth. Liver biopsies were taken upon laparotomy, after three and six weeks. Gene expression profiles were obtained using porcine-specific oligonucleotide microarrays. Immunohistochemical staining was performed and a proliferative index was assessed. Results. More differentially expressed genes were associated with the regulation of ECM in the resection group compared to the sham and control groups. Secreted protein acidic and rich in cysteine (SPARC) and collagen 1, alpha 2 (COL1A2) were both upregulated in the early phase of liver regeneration, validated by immunopositive cells during the remodelling phase of liver regeneration. A broadened connective tissue was demonstrated by Masson's Trichrome staining, and an immunohistochemical staining against pan-Cytokeratin (pan-CK) demonstrated a distinct pattern of migrating cells, followed by proliferating cell nuclear antigen (PCNA) positive nuclei. Conclusions. The present study demonstrates both a distinct pattern of PCNA positive nuclei and a deposition of ECM proteins in the remodelling phase of liver regeneration. Hindawi Publishing Corporation 2015 2015-07-09 /pmc/articles/PMC4512564/ /pubmed/26240819 http://dx.doi.org/10.1155/2015/248920 Text en Copyright © 2015 Ingvild Engdal Nygård et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Nygård, Ingvild Engdal
Mortensen, Kim Erlend
Hedegaard, Jakob
Conley, Lene Nagstrup
Bendixen, Christian
Sveinbjørnsson, Baldur
Revhaug, Arthur
Tissue Remodelling following Resection of Porcine Liver
title Tissue Remodelling following Resection of Porcine Liver
title_full Tissue Remodelling following Resection of Porcine Liver
title_fullStr Tissue Remodelling following Resection of Porcine Liver
title_full_unstemmed Tissue Remodelling following Resection of Porcine Liver
title_short Tissue Remodelling following Resection of Porcine Liver
title_sort tissue remodelling following resection of porcine liver
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4512564/
https://www.ncbi.nlm.nih.gov/pubmed/26240819
http://dx.doi.org/10.1155/2015/248920
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