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Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network
Pericytes enveloping the endothelium play an important role in the physiology and pathology of microvessels, especially in vessel maturation and stabilization. However, our understanding of fundamental pericyte biology is limited by the lack of a robust in vitro model system that allows researchers...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4512698/ https://www.ncbi.nlm.nih.gov/pubmed/26204526 http://dx.doi.org/10.1371/journal.pone.0133880 |
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author | Kim, Jaerim Chung, Minhwan Kim, Sudong Jo, Dong Hyun Kim, Jeong Hun Jeon, Noo Li |
author_facet | Kim, Jaerim Chung, Minhwan Kim, Sudong Jo, Dong Hyun Kim, Jeong Hun Jeon, Noo Li |
author_sort | Kim, Jaerim |
collection | PubMed |
description | Pericytes enveloping the endothelium play an important role in the physiology and pathology of microvessels, especially in vessel maturation and stabilization. However, our understanding of fundamental pericyte biology is limited by the lack of a robust in vitro model system that allows researchers to evaluate the interactions among multiple cell types in perfusable blood vessels. The present work describes a microfluidic platform that can be used to investigate interactions between pericytes and endothelial cells (ECs) during the sprouting, growth, and maturation steps of neovessel formation. A mixture of ECs and pericytes was attached to the side of a pre-patterned three dimensional fibrin matrix and allowed to sprout across the matrix. The effects of intact coverage and EC maturation by the pericytes on the perfused EC network were confirmed using a confocal microscope. Compared with EC monoculture conditions, EC-pericyte co-cultured vessels showed a significant reduction in diameter, increased numbers of junctions and branches and decreased permeability. In response to biochemical factors, ECs and pericytes in the platform showed the similar features with previous reports from in vivo experiments, thus reflect various pathophysiological conditions of in vivo microvessels. Taken together, these results support the physiological relevancy of our three-dimensional microfluidic culture system but also that the system can be used to screen drug effect on EC-pericyte biology. |
format | Online Article Text |
id | pubmed-4512698 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45126982015-07-24 Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network Kim, Jaerim Chung, Minhwan Kim, Sudong Jo, Dong Hyun Kim, Jeong Hun Jeon, Noo Li PLoS One Research Article Pericytes enveloping the endothelium play an important role in the physiology and pathology of microvessels, especially in vessel maturation and stabilization. However, our understanding of fundamental pericyte biology is limited by the lack of a robust in vitro model system that allows researchers to evaluate the interactions among multiple cell types in perfusable blood vessels. The present work describes a microfluidic platform that can be used to investigate interactions between pericytes and endothelial cells (ECs) during the sprouting, growth, and maturation steps of neovessel formation. A mixture of ECs and pericytes was attached to the side of a pre-patterned three dimensional fibrin matrix and allowed to sprout across the matrix. The effects of intact coverage and EC maturation by the pericytes on the perfused EC network were confirmed using a confocal microscope. Compared with EC monoculture conditions, EC-pericyte co-cultured vessels showed a significant reduction in diameter, increased numbers of junctions and branches and decreased permeability. In response to biochemical factors, ECs and pericytes in the platform showed the similar features with previous reports from in vivo experiments, thus reflect various pathophysiological conditions of in vivo microvessels. Taken together, these results support the physiological relevancy of our three-dimensional microfluidic culture system but also that the system can be used to screen drug effect on EC-pericyte biology. Public Library of Science 2015-07-23 /pmc/articles/PMC4512698/ /pubmed/26204526 http://dx.doi.org/10.1371/journal.pone.0133880 Text en © 2015 Kim et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kim, Jaerim Chung, Minhwan Kim, Sudong Jo, Dong Hyun Kim, Jeong Hun Jeon, Noo Li Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network |
title | Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network |
title_full | Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network |
title_fullStr | Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network |
title_full_unstemmed | Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network |
title_short | Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network |
title_sort | engineering of a biomimetic pericyte-covered 3d microvascular network |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4512698/ https://www.ncbi.nlm.nih.gov/pubmed/26204526 http://dx.doi.org/10.1371/journal.pone.0133880 |
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