Cargando…

Coenzyme Q(10) and α-lipoic acid: antioxidant and pro-oxidant effects in plasma and peripheral blood lymphocytes of supplemented subjects

Reactive oxygen species not only cause damage but also have a physiological role in the protection against pathogens and in cell signalling. Mitochondrial nutrients, such as coenzyme Q(10) and α-lipoic acid, beside their acknowledged antioxidant activities, show interesting features in relation to t...

Descripción completa

Detalles Bibliográficos
Autores principales: Silvestri, Sonia, Orlando, Patrick, Armeni, Tatiana, Padella, Lucia, Brugè, Francesca, Seddaiu, Giovanna, Littarru, Gian Paolo, Tiano, Luca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: the Society for Free Radical Research Japan 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4512890/
https://www.ncbi.nlm.nih.gov/pubmed/26236096
http://dx.doi.org/10.3164/jcbn.14-130
_version_ 1782382560413745152
author Silvestri, Sonia
Orlando, Patrick
Armeni, Tatiana
Padella, Lucia
Brugè, Francesca
Seddaiu, Giovanna
Littarru, Gian Paolo
Tiano, Luca
author_facet Silvestri, Sonia
Orlando, Patrick
Armeni, Tatiana
Padella, Lucia
Brugè, Francesca
Seddaiu, Giovanna
Littarru, Gian Paolo
Tiano, Luca
author_sort Silvestri, Sonia
collection PubMed
description Reactive oxygen species not only cause damage but also have a physiological role in the protection against pathogens and in cell signalling. Mitochondrial nutrients, such as coenzyme Q(10) and α-lipoic acid, beside their acknowledged antioxidant activities, show interesting features in relation to their redox state and consequent biological activity. In this study, we tested whether oral supplementation with 200 mg/day of coenzyme Q(10) alone or in association with 200 mg/die of α-lipoic acid for 15 days on 16 healthy subjects was able to modulate the oxidative status into different compartments (plasma and cells), in basal condition and following an oxidative insult in peripheral blood lymphocytes exposed in vitro to H(2)O(2). Data have shown that tested compounds produced antioxidant and bioenergetic effects improving oxidative status of the lipid compartment and mitochondrial functionality in peripheral blood lymphocytes. Simultaneously, an increased intracellular reactive oxygen species level was observed, although they did not lead to enhanced DNA oxidative damage. Coenzyme Q(10) and α-lipoic acid produced beneficial effects also steering intracellular redox poise toward a pro-oxidant environment. In contrast with other antioxidant molecules, pro-oxidant activities of tested mitochondrial nutrients and consequent oxidant mediated signalling, could have important implications in promoting adaptive response to oxidative stress.
format Online
Article
Text
id pubmed-4512890
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher the Society for Free Radical Research Japan
record_format MEDLINE/PubMed
spelling pubmed-45128902015-07-31 Coenzyme Q(10) and α-lipoic acid: antioxidant and pro-oxidant effects in plasma and peripheral blood lymphocytes of supplemented subjects Silvestri, Sonia Orlando, Patrick Armeni, Tatiana Padella, Lucia Brugè, Francesca Seddaiu, Giovanna Littarru, Gian Paolo Tiano, Luca J Clin Biochem Nutr Original Article Reactive oxygen species not only cause damage but also have a physiological role in the protection against pathogens and in cell signalling. Mitochondrial nutrients, such as coenzyme Q(10) and α-lipoic acid, beside their acknowledged antioxidant activities, show interesting features in relation to their redox state and consequent biological activity. In this study, we tested whether oral supplementation with 200 mg/day of coenzyme Q(10) alone or in association with 200 mg/die of α-lipoic acid for 15 days on 16 healthy subjects was able to modulate the oxidative status into different compartments (plasma and cells), in basal condition and following an oxidative insult in peripheral blood lymphocytes exposed in vitro to H(2)O(2). Data have shown that tested compounds produced antioxidant and bioenergetic effects improving oxidative status of the lipid compartment and mitochondrial functionality in peripheral blood lymphocytes. Simultaneously, an increased intracellular reactive oxygen species level was observed, although they did not lead to enhanced DNA oxidative damage. Coenzyme Q(10) and α-lipoic acid produced beneficial effects also steering intracellular redox poise toward a pro-oxidant environment. In contrast with other antioxidant molecules, pro-oxidant activities of tested mitochondrial nutrients and consequent oxidant mediated signalling, could have important implications in promoting adaptive response to oxidative stress. the Society for Free Radical Research Japan 2015-07 2015-04-16 /pmc/articles/PMC4512890/ /pubmed/26236096 http://dx.doi.org/10.3164/jcbn.14-130 Text en Copyright © 2015 JCBN This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Silvestri, Sonia
Orlando, Patrick
Armeni, Tatiana
Padella, Lucia
Brugè, Francesca
Seddaiu, Giovanna
Littarru, Gian Paolo
Tiano, Luca
Coenzyme Q(10) and α-lipoic acid: antioxidant and pro-oxidant effects in plasma and peripheral blood lymphocytes of supplemented subjects
title Coenzyme Q(10) and α-lipoic acid: antioxidant and pro-oxidant effects in plasma and peripheral blood lymphocytes of supplemented subjects
title_full Coenzyme Q(10) and α-lipoic acid: antioxidant and pro-oxidant effects in plasma and peripheral blood lymphocytes of supplemented subjects
title_fullStr Coenzyme Q(10) and α-lipoic acid: antioxidant and pro-oxidant effects in plasma and peripheral blood lymphocytes of supplemented subjects
title_full_unstemmed Coenzyme Q(10) and α-lipoic acid: antioxidant and pro-oxidant effects in plasma and peripheral blood lymphocytes of supplemented subjects
title_short Coenzyme Q(10) and α-lipoic acid: antioxidant and pro-oxidant effects in plasma and peripheral blood lymphocytes of supplemented subjects
title_sort coenzyme q(10) and α-lipoic acid: antioxidant and pro-oxidant effects in plasma and peripheral blood lymphocytes of supplemented subjects
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4512890/
https://www.ncbi.nlm.nih.gov/pubmed/26236096
http://dx.doi.org/10.3164/jcbn.14-130
work_keys_str_mv AT silvestrisonia coenzymeq10andalipoicacidantioxidantandprooxidanteffectsinplasmaandperipheralbloodlymphocytesofsupplementedsubjects
AT orlandopatrick coenzymeq10andalipoicacidantioxidantandprooxidanteffectsinplasmaandperipheralbloodlymphocytesofsupplementedsubjects
AT armenitatiana coenzymeq10andalipoicacidantioxidantandprooxidanteffectsinplasmaandperipheralbloodlymphocytesofsupplementedsubjects
AT padellalucia coenzymeq10andalipoicacidantioxidantandprooxidanteffectsinplasmaandperipheralbloodlymphocytesofsupplementedsubjects
AT brugefrancesca coenzymeq10andalipoicacidantioxidantandprooxidanteffectsinplasmaandperipheralbloodlymphocytesofsupplementedsubjects
AT seddaiugiovanna coenzymeq10andalipoicacidantioxidantandprooxidanteffectsinplasmaandperipheralbloodlymphocytesofsupplementedsubjects
AT littarrugianpaolo coenzymeq10andalipoicacidantioxidantandprooxidanteffectsinplasmaandperipheralbloodlymphocytesofsupplementedsubjects
AT tianoluca coenzymeq10andalipoicacidantioxidantandprooxidanteffectsinplasmaandperipheralbloodlymphocytesofsupplementedsubjects