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Multimeric Near IR–MR Contrast Agent for Multimodal In Vivo Imaging

[Image: see text] Multiple imaging modalities are often required for in vivo imaging applications that require both high probe sensitivity and excellent spatial and temporal resolution. In particular, MR and optical imaging are an attractive combination that can be used to determine both molecular a...

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Autores principales: Harrison, Victoria S. R., Carney, Christiane E., MacRenaris, Keith W., Waters, Emily A., Meade, Thomas J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2015
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4512902/
https://www.ncbi.nlm.nih.gov/pubmed/26083313
http://dx.doi.org/10.1021/jacs.5b04509
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author Harrison, Victoria S. R.
Carney, Christiane E.
MacRenaris, Keith W.
Waters, Emily A.
Meade, Thomas J.
author_facet Harrison, Victoria S. R.
Carney, Christiane E.
MacRenaris, Keith W.
Waters, Emily A.
Meade, Thomas J.
author_sort Harrison, Victoria S. R.
collection PubMed
description [Image: see text] Multiple imaging modalities are often required for in vivo imaging applications that require both high probe sensitivity and excellent spatial and temporal resolution. In particular, MR and optical imaging are an attractive combination that can be used to determine both molecular and anatomical information. Herein, we describe the synthesis and in vivo testing of two multimeric NIR–MR contrast agents that contain three Gd(III) chelates and an IR-783 dye moiety. One agent contains a PEG linker and the other a short alkyl linker. These agents label cells with extraordinary efficacy and can be detected in vivo using both imaging modalities. Biodistribution of the PEGylated agent shows observable fluorescence in xenograft MCF7 tumors and renal clearance by MR imaging.
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spelling pubmed-45129022015-07-23 Multimeric Near IR–MR Contrast Agent for Multimodal In Vivo Imaging Harrison, Victoria S. R. Carney, Christiane E. MacRenaris, Keith W. Waters, Emily A. Meade, Thomas J. J Am Chem Soc [Image: see text] Multiple imaging modalities are often required for in vivo imaging applications that require both high probe sensitivity and excellent spatial and temporal resolution. In particular, MR and optical imaging are an attractive combination that can be used to determine both molecular and anatomical information. Herein, we describe the synthesis and in vivo testing of two multimeric NIR–MR contrast agents that contain three Gd(III) chelates and an IR-783 dye moiety. One agent contains a PEG linker and the other a short alkyl linker. These agents label cells with extraordinary efficacy and can be detected in vivo using both imaging modalities. Biodistribution of the PEGylated agent shows observable fluorescence in xenograft MCF7 tumors and renal clearance by MR imaging. American Chemical Society 2015-06-17 2015-07-22 /pmc/articles/PMC4512902/ /pubmed/26083313 http://dx.doi.org/10.1021/jacs.5b04509 Text en Copyright © 2015 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Harrison, Victoria S. R.
Carney, Christiane E.
MacRenaris, Keith W.
Waters, Emily A.
Meade, Thomas J.
Multimeric Near IR–MR Contrast Agent for Multimodal In Vivo Imaging
title Multimeric Near IR–MR Contrast Agent for Multimodal In Vivo Imaging
title_full Multimeric Near IR–MR Contrast Agent for Multimodal In Vivo Imaging
title_fullStr Multimeric Near IR–MR Contrast Agent for Multimodal In Vivo Imaging
title_full_unstemmed Multimeric Near IR–MR Contrast Agent for Multimodal In Vivo Imaging
title_short Multimeric Near IR–MR Contrast Agent for Multimodal In Vivo Imaging
title_sort multimeric near ir–mr contrast agent for multimodal in vivo imaging
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4512902/
https://www.ncbi.nlm.nih.gov/pubmed/26083313
http://dx.doi.org/10.1021/jacs.5b04509
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