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CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 Genes in Trypanosoma cruzi Reveals Their Role in Flagellar Attachment

Trypanosoma cruzi is the etiologic agent of Chagas disease, and current methods for its genetic manipulation have been highly inefficient. We report here the use of the CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 (CRISPR-associated gene 9) system for disrupting genes in t...

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Detalles Bibliográficos
Autores principales:	Lander, Noelia, Li, Zhu-Hong, Niyogi, Sayantanee, Docampo, Roberto
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society of Microbiology 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513075/ https://www.ncbi.nlm.nih.gov/pubmed/26199333 http://dx.doi.org/10.1128/mBio.01012-15

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