Cargando…

A lipidomic approach to the study of human CD4(+) T lymphocytes in multiple sclerosis

BACKGROUND: Lipids play different important roles in central nervous system so that dysregulation of lipid pathways has been implicated in a growing number of neurodegenerative disorders including multiple sclerosis (MS). MS is the most prevalent autoimmune disorder of the central nervous system, wi...

Descripción completa

Detalles Bibliográficos
Autores principales: Vergara, Daniele, D’Alessandro, Michele, Rizzello, Antonia, De Riccardis, Lidia, Lunetti, Paola, Del Boccio, Piero, De Robertis, Francesca, Trianni, Giorgio, Maffia, Michele, Giudetti, Anna M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513631/
https://www.ncbi.nlm.nih.gov/pubmed/26205308
http://dx.doi.org/10.1186/s12868-015-0183-1
_version_ 1782382675412123648
author Vergara, Daniele
D’Alessandro, Michele
Rizzello, Antonia
De Riccardis, Lidia
Lunetti, Paola
Del Boccio, Piero
De Robertis, Francesca
Trianni, Giorgio
Maffia, Michele
Giudetti, Anna M
author_facet Vergara, Daniele
D’Alessandro, Michele
Rizzello, Antonia
De Riccardis, Lidia
Lunetti, Paola
Del Boccio, Piero
De Robertis, Francesca
Trianni, Giorgio
Maffia, Michele
Giudetti, Anna M
author_sort Vergara, Daniele
collection PubMed
description BACKGROUND: Lipids play different important roles in central nervous system so that dysregulation of lipid pathways has been implicated in a growing number of neurodegenerative disorders including multiple sclerosis (MS). MS is the most prevalent autoimmune disorder of the central nervous system, with neurological symptoms caused by inflammation and demyelination. In this study, a lipidomic analysis was performed for the rapid profile of CD4(+) T lymphocytes from MS patient and control samples in an untargeted approach. METHODS: A matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry based approach was used for the analysis of lipid extracts using 9-aminoacridine as matrix. Lipids were analyzed in negative mode and selected species fragmented using MALDI tandem mass spectrometry for their structural assignments. RESULTS: The analysis reveals some modifications in the phospholipid pattern of MS CD4(+) T lymphocytes with respect to healthy controls with a significant increase of cardiolipin species in MS samples. CONCLUSIONS: These results demonstrate the feasibility of a MALDI-TOF approach for the analysis of CD4(+) lipid extracts and suggest how alterations in the lipid metabolism characterized lymphocytes of MS patients.
format Online
Article
Text
id pubmed-4513631
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-45136312015-07-25 A lipidomic approach to the study of human CD4(+) T lymphocytes in multiple sclerosis Vergara, Daniele D’Alessandro, Michele Rizzello, Antonia De Riccardis, Lidia Lunetti, Paola Del Boccio, Piero De Robertis, Francesca Trianni, Giorgio Maffia, Michele Giudetti, Anna M BMC Neurosci Research Article BACKGROUND: Lipids play different important roles in central nervous system so that dysregulation of lipid pathways has been implicated in a growing number of neurodegenerative disorders including multiple sclerosis (MS). MS is the most prevalent autoimmune disorder of the central nervous system, with neurological symptoms caused by inflammation and demyelination. In this study, a lipidomic analysis was performed for the rapid profile of CD4(+) T lymphocytes from MS patient and control samples in an untargeted approach. METHODS: A matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry based approach was used for the analysis of lipid extracts using 9-aminoacridine as matrix. Lipids were analyzed in negative mode and selected species fragmented using MALDI tandem mass spectrometry for their structural assignments. RESULTS: The analysis reveals some modifications in the phospholipid pattern of MS CD4(+) T lymphocytes with respect to healthy controls with a significant increase of cardiolipin species in MS samples. CONCLUSIONS: These results demonstrate the feasibility of a MALDI-TOF approach for the analysis of CD4(+) lipid extracts and suggest how alterations in the lipid metabolism characterized lymphocytes of MS patients. BioMed Central 2015-07-24 /pmc/articles/PMC4513631/ /pubmed/26205308 http://dx.doi.org/10.1186/s12868-015-0183-1 Text en © Vergara et al. 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Vergara, Daniele
D’Alessandro, Michele
Rizzello, Antonia
De Riccardis, Lidia
Lunetti, Paola
Del Boccio, Piero
De Robertis, Francesca
Trianni, Giorgio
Maffia, Michele
Giudetti, Anna M
A lipidomic approach to the study of human CD4(+) T lymphocytes in multiple sclerosis
title A lipidomic approach to the study of human CD4(+) T lymphocytes in multiple sclerosis
title_full A lipidomic approach to the study of human CD4(+) T lymphocytes in multiple sclerosis
title_fullStr A lipidomic approach to the study of human CD4(+) T lymphocytes in multiple sclerosis
title_full_unstemmed A lipidomic approach to the study of human CD4(+) T lymphocytes in multiple sclerosis
title_short A lipidomic approach to the study of human CD4(+) T lymphocytes in multiple sclerosis
title_sort lipidomic approach to the study of human cd4(+) t lymphocytes in multiple sclerosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513631/
https://www.ncbi.nlm.nih.gov/pubmed/26205308
http://dx.doi.org/10.1186/s12868-015-0183-1
work_keys_str_mv AT vergaradaniele alipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT dalessandromichele alipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT rizzelloantonia alipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT dericcardislidia alipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT lunettipaola alipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT delbocciopiero alipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT derobertisfrancesca alipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT triannigiorgio alipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT maffiamichele alipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT giudettiannam alipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT vergaradaniele lipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT dalessandromichele lipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT rizzelloantonia lipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT dericcardislidia lipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT lunettipaola lipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT delbocciopiero lipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT derobertisfrancesca lipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT triannigiorgio lipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT maffiamichele lipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis
AT giudettiannam lipidomicapproachtothestudyofhumancd4tlymphocytesinmultiplesclerosis