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A cis-encoded sRNA, Hfq and mRNA secondary structure act independently to suppress IS200 transposition

IS200 is found throughout Enterobacteriaceae and transposes at a notoriously low frequency. In addition to the transposase protein (TnpA), IS200 encodes an uncharacterized Hfq-binding sRNA that is encoded opposite to the tnpA 5'UTR. In the current work we asked if this sRNA represses tnpA expre...

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Detalles Bibliográficos
Autores principales: Ellis, Michael J., Trussler, Ryan S., Haniford, David B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
RNA
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513863/
https://www.ncbi.nlm.nih.gov/pubmed/26044710
http://dx.doi.org/10.1093/nar/gkv584
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author Ellis, Michael J.
Trussler, Ryan S.
Haniford, David B.
author_facet Ellis, Michael J.
Trussler, Ryan S.
Haniford, David B.
author_sort Ellis, Michael J.
collection PubMed
description IS200 is found throughout Enterobacteriaceae and transposes at a notoriously low frequency. In addition to the transposase protein (TnpA), IS200 encodes an uncharacterized Hfq-binding sRNA that is encoded opposite to the tnpA 5'UTR. In the current work we asked if this sRNA represses tnpA expression. We show here that the IS200 sRNA (named art200 for antisense regulator of transposase IS200) basepairs with tnpA to inhibit translation initiation. Unexpectedly, art200-tnpA pairing is limited to 40 bp, despite 90 nt of perfect complementarity. Additionally, we show that Hfq and RNA secondary structure in the tnpA 5'UTR each repress tnpA expression in an art200-independent manner. Finally, we show that disrupting translational control of tnpA expression leads to increased IS200 transposition in E. coli. The current work provides new mechanistic insight into why IS200 transposition is so strongly suppressed. The possibility of art200 acting in trans to regulate a yet-unidentified target is discussed as well as potential applications of the IS200 system for designing novel riboregulators.
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spelling pubmed-45138632015-07-27 A cis-encoded sRNA, Hfq and mRNA secondary structure act independently to suppress IS200 transposition Ellis, Michael J. Trussler, Ryan S. Haniford, David B. Nucleic Acids Res RNA IS200 is found throughout Enterobacteriaceae and transposes at a notoriously low frequency. In addition to the transposase protein (TnpA), IS200 encodes an uncharacterized Hfq-binding sRNA that is encoded opposite to the tnpA 5'UTR. In the current work we asked if this sRNA represses tnpA expression. We show here that the IS200 sRNA (named art200 for antisense regulator of transposase IS200) basepairs with tnpA to inhibit translation initiation. Unexpectedly, art200-tnpA pairing is limited to 40 bp, despite 90 nt of perfect complementarity. Additionally, we show that Hfq and RNA secondary structure in the tnpA 5'UTR each repress tnpA expression in an art200-independent manner. Finally, we show that disrupting translational control of tnpA expression leads to increased IS200 transposition in E. coli. The current work provides new mechanistic insight into why IS200 transposition is so strongly suppressed. The possibility of art200 acting in trans to regulate a yet-unidentified target is discussed as well as potential applications of the IS200 system for designing novel riboregulators. Oxford University Press 2015-07-27 2015-06-04 /pmc/articles/PMC4513863/ /pubmed/26044710 http://dx.doi.org/10.1093/nar/gkv584 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle RNA
Ellis, Michael J.
Trussler, Ryan S.
Haniford, David B.
A cis-encoded sRNA, Hfq and mRNA secondary structure act independently to suppress IS200 transposition
title A cis-encoded sRNA, Hfq and mRNA secondary structure act independently to suppress IS200 transposition
title_full A cis-encoded sRNA, Hfq and mRNA secondary structure act independently to suppress IS200 transposition
title_fullStr A cis-encoded sRNA, Hfq and mRNA secondary structure act independently to suppress IS200 transposition
title_full_unstemmed A cis-encoded sRNA, Hfq and mRNA secondary structure act independently to suppress IS200 transposition
title_short A cis-encoded sRNA, Hfq and mRNA secondary structure act independently to suppress IS200 transposition
title_sort cis-encoded srna, hfq and mrna secondary structure act independently to suppress is200 transposition
topic RNA
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4513863/
https://www.ncbi.nlm.nih.gov/pubmed/26044710
http://dx.doi.org/10.1093/nar/gkv584
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