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Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant
The Neisseria meningitidis outer membrane protein PorA from a Chilean strain was purified as a recombinant protein. PorA mixed with AbISCO induced bactericidal antibodies against N. meningitidis in mice. When PorA was fused to the Helicobacter pylori HpaA antigen gene, the specific response against...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514328/ https://www.ncbi.nlm.nih.gov/pubmed/25750999 http://dx.doi.org/10.1080/21645515.2015.1011011 |
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author | Vasquez, Abel E Manzo, Ricardo A Soto, Daniel A Barrientos, Magaly J Maldonado, Aurora E Mosqueira, Macarena Avila, Anastasia Touma, Jorge Bruce, Elsa Harris, Paul R Venegas, Alejandro |
author_facet | Vasquez, Abel E Manzo, Ricardo A Soto, Daniel A Barrientos, Magaly J Maldonado, Aurora E Mosqueira, Macarena Avila, Anastasia Touma, Jorge Bruce, Elsa Harris, Paul R Venegas, Alejandro |
author_sort | Vasquez, Abel E |
collection | PubMed |
description | The Neisseria meningitidis outer membrane protein PorA from a Chilean strain was purified as a recombinant protein. PorA mixed with AbISCO induced bactericidal antibodies against N. meningitidis in mice. When PorA was fused to the Helicobacter pylori HpaA antigen gene, the specific response against H. pylori protein increased. Splenocytes from PorA-immunized mice were stimulated with PorA, and an increase in the secretion of IL-4 was observed compared with that of IFN-γ. Moreover, in an immunoglobulin sub-typing analysis, a substantially higher IgG(1) level was found compared with IgG(2a) levels, suggesting a Th2-type immune response. This study revealed a peculiar behavior of the purified recombinant PorA protein per se in the absence of AbISCO as an adjuvant. Therefore, the resistance of PorA to proteolytic enzymes, such as those in the gastrointestinal tract, was analyzed, because this is an important feature for an oral protein adjuvant. Finally, we found that PorA fused to the H. pylori HpaA antigen, when expressed in Lactococcus lactis and administered orally, could enhance the antibody response against the HpaA antigen approximately 3 fold. These observations strongly suggest that PorA behaves as an effective oral adjuvant. |
format | Online Article Text |
id | pubmed-4514328 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-45143282016-02-03 Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant Vasquez, Abel E Manzo, Ricardo A Soto, Daniel A Barrientos, Magaly J Maldonado, Aurora E Mosqueira, Macarena Avila, Anastasia Touma, Jorge Bruce, Elsa Harris, Paul R Venegas, Alejandro Hum Vaccin Immunother Research Paper The Neisseria meningitidis outer membrane protein PorA from a Chilean strain was purified as a recombinant protein. PorA mixed with AbISCO induced bactericidal antibodies against N. meningitidis in mice. When PorA was fused to the Helicobacter pylori HpaA antigen gene, the specific response against H. pylori protein increased. Splenocytes from PorA-immunized mice were stimulated with PorA, and an increase in the secretion of IL-4 was observed compared with that of IFN-γ. Moreover, in an immunoglobulin sub-typing analysis, a substantially higher IgG(1) level was found compared with IgG(2a) levels, suggesting a Th2-type immune response. This study revealed a peculiar behavior of the purified recombinant PorA protein per se in the absence of AbISCO as an adjuvant. Therefore, the resistance of PorA to proteolytic enzymes, such as those in the gastrointestinal tract, was analyzed, because this is an important feature for an oral protein adjuvant. Finally, we found that PorA fused to the H. pylori HpaA antigen, when expressed in Lactococcus lactis and administered orally, could enhance the antibody response against the HpaA antigen approximately 3 fold. These observations strongly suggest that PorA behaves as an effective oral adjuvant. Taylor & Francis 2015-03-09 /pmc/articles/PMC4514328/ /pubmed/25750999 http://dx.doi.org/10.1080/21645515.2015.1011011 Text en © 2015 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. |
spellingShingle | Research Paper Vasquez, Abel E Manzo, Ricardo A Soto, Daniel A Barrientos, Magaly J Maldonado, Aurora E Mosqueira, Macarena Avila, Anastasia Touma, Jorge Bruce, Elsa Harris, Paul R Venegas, Alejandro Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant |
title | Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant |
title_full | Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant |
title_fullStr | Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant |
title_full_unstemmed | Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant |
title_short | Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant |
title_sort | oral administration of recombinant neisseria meningitidis pora genetically fused to h. pylori hpaa antigen increases antibody levels in mouse serum, suggesting that pora behaves as a putative adjuvant |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514328/ https://www.ncbi.nlm.nih.gov/pubmed/25750999 http://dx.doi.org/10.1080/21645515.2015.1011011 |
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