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Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant

The Neisseria meningitidis outer membrane protein PorA from a Chilean strain was purified as a recombinant protein. PorA mixed with AbISCO induced bactericidal antibodies against N. meningitidis in mice. When PorA was fused to the Helicobacter pylori HpaA antigen gene, the specific response against...

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Autores principales: Vasquez, Abel E, Manzo, Ricardo A, Soto, Daniel A, Barrientos, Magaly J, Maldonado, Aurora E, Mosqueira, Macarena, Avila, Anastasia, Touma, Jorge, Bruce, Elsa, Harris, Paul R, Venegas, Alejandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514328/
https://www.ncbi.nlm.nih.gov/pubmed/25750999
http://dx.doi.org/10.1080/21645515.2015.1011011
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author Vasquez, Abel E
Manzo, Ricardo A
Soto, Daniel A
Barrientos, Magaly J
Maldonado, Aurora E
Mosqueira, Macarena
Avila, Anastasia
Touma, Jorge
Bruce, Elsa
Harris, Paul R
Venegas, Alejandro
author_facet Vasquez, Abel E
Manzo, Ricardo A
Soto, Daniel A
Barrientos, Magaly J
Maldonado, Aurora E
Mosqueira, Macarena
Avila, Anastasia
Touma, Jorge
Bruce, Elsa
Harris, Paul R
Venegas, Alejandro
author_sort Vasquez, Abel E
collection PubMed
description The Neisseria meningitidis outer membrane protein PorA from a Chilean strain was purified as a recombinant protein. PorA mixed with AbISCO induced bactericidal antibodies against N. meningitidis in mice. When PorA was fused to the Helicobacter pylori HpaA antigen gene, the specific response against H. pylori protein increased. Splenocytes from PorA-immunized mice were stimulated with PorA, and an increase in the secretion of IL-4 was observed compared with that of IFN-γ. Moreover, in an immunoglobulin sub-typing analysis, a substantially higher IgG(1) level was found compared with IgG(2a) levels, suggesting a Th2-type immune response. This study revealed a peculiar behavior of the purified recombinant PorA protein per se in the absence of AbISCO as an adjuvant. Therefore, the resistance of PorA to proteolytic enzymes, such as those in the gastrointestinal tract, was analyzed, because this is an important feature for an oral protein adjuvant. Finally, we found that PorA fused to the H. pylori HpaA antigen, when expressed in Lactococcus lactis and administered orally, could enhance the antibody response against the HpaA antigen approximately 3 fold. These observations strongly suggest that PorA behaves as an effective oral adjuvant.
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spelling pubmed-45143282016-02-03 Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant Vasquez, Abel E Manzo, Ricardo A Soto, Daniel A Barrientos, Magaly J Maldonado, Aurora E Mosqueira, Macarena Avila, Anastasia Touma, Jorge Bruce, Elsa Harris, Paul R Venegas, Alejandro Hum Vaccin Immunother Research Paper The Neisseria meningitidis outer membrane protein PorA from a Chilean strain was purified as a recombinant protein. PorA mixed with AbISCO induced bactericidal antibodies against N. meningitidis in mice. When PorA was fused to the Helicobacter pylori HpaA antigen gene, the specific response against H. pylori protein increased. Splenocytes from PorA-immunized mice were stimulated with PorA, and an increase in the secretion of IL-4 was observed compared with that of IFN-γ. Moreover, in an immunoglobulin sub-typing analysis, a substantially higher IgG(1) level was found compared with IgG(2a) levels, suggesting a Th2-type immune response. This study revealed a peculiar behavior of the purified recombinant PorA protein per se in the absence of AbISCO as an adjuvant. Therefore, the resistance of PorA to proteolytic enzymes, such as those in the gastrointestinal tract, was analyzed, because this is an important feature for an oral protein adjuvant. Finally, we found that PorA fused to the H. pylori HpaA antigen, when expressed in Lactococcus lactis and administered orally, could enhance the antibody response against the HpaA antigen approximately 3 fold. These observations strongly suggest that PorA behaves as an effective oral adjuvant. Taylor & Francis 2015-03-09 /pmc/articles/PMC4514328/ /pubmed/25750999 http://dx.doi.org/10.1080/21645515.2015.1011011 Text en © 2015 The Author(s). Published with license by Taylor & Francis Group, LLC http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted.
spellingShingle Research Paper
Vasquez, Abel E
Manzo, Ricardo A
Soto, Daniel A
Barrientos, Magaly J
Maldonado, Aurora E
Mosqueira, Macarena
Avila, Anastasia
Touma, Jorge
Bruce, Elsa
Harris, Paul R
Venegas, Alejandro
Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant
title Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant
title_full Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant
title_fullStr Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant
title_full_unstemmed Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant
title_short Oral administration of recombinant Neisseria meningitidis PorA genetically fused to H. pylori HpaA antigen increases antibody levels in mouse serum, suggesting that PorA behaves as a putative adjuvant
title_sort oral administration of recombinant neisseria meningitidis pora genetically fused to h. pylori hpaa antigen increases antibody levels in mouse serum, suggesting that pora behaves as a putative adjuvant
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514328/
https://www.ncbi.nlm.nih.gov/pubmed/25750999
http://dx.doi.org/10.1080/21645515.2015.1011011
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