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The Corn Smut (‘Huitlacoche’) as a New Platform for Oral Vaccines

The development of new alternative platforms for subunit vaccine production is a priority in the biomedical field. In this study, Ustilago maydis, the causal agent of common corn smut or ‘huitlacoche’has been genetically engineered to assess expression and immunogenicity of the B subunit of the chol...

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Autores principales: Juárez-Montiel, Margarita, Romero-Maldonado, Andrea, Monreal-Escalante, Elizabeth, Becerra-Flora, Alicia, Korban, Schuyler S., Rosales-Mendoza, Sergio, Jiménez-Bremont, Juan Francisco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514630/
https://www.ncbi.nlm.nih.gov/pubmed/26207365
http://dx.doi.org/10.1371/journal.pone.0133535
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author Juárez-Montiel, Margarita
Romero-Maldonado, Andrea
Monreal-Escalante, Elizabeth
Becerra-Flora, Alicia
Korban, Schuyler S.
Rosales-Mendoza, Sergio
Jiménez-Bremont, Juan Francisco
author_facet Juárez-Montiel, Margarita
Romero-Maldonado, Andrea
Monreal-Escalante, Elizabeth
Becerra-Flora, Alicia
Korban, Schuyler S.
Rosales-Mendoza, Sergio
Jiménez-Bremont, Juan Francisco
author_sort Juárez-Montiel, Margarita
collection PubMed
description The development of new alternative platforms for subunit vaccine production is a priority in the biomedical field. In this study, Ustilago maydis, the causal agent of common corn smut or ‘huitlacoche’has been genetically engineered to assess expression and immunogenicity of the B subunit of the cholera toxin (CTB), a relevant immunomodulatory agent in vaccinology. An oligomeric CTB recombinant protein was expressed in corn smut galls at levels of up to 1.3 mg g-1 dry weight (0.8% of the total soluble protein). Mice orally immunized with ‘huitlacoche’-derived CTB showed significant humoral responses that were well-correlated with protection against challenge with the cholera toxin (CT). These findings demonstrate the feasibility of using edible corn smut as a safe, effective, and low-cost platform for production and delivery of a subunit oral vaccine. The implications of this platform in the area of molecular pharming are discussed.
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spelling pubmed-45146302015-07-29 The Corn Smut (‘Huitlacoche’) as a New Platform for Oral Vaccines Juárez-Montiel, Margarita Romero-Maldonado, Andrea Monreal-Escalante, Elizabeth Becerra-Flora, Alicia Korban, Schuyler S. Rosales-Mendoza, Sergio Jiménez-Bremont, Juan Francisco PLoS One Research Article The development of new alternative platforms for subunit vaccine production is a priority in the biomedical field. In this study, Ustilago maydis, the causal agent of common corn smut or ‘huitlacoche’has been genetically engineered to assess expression and immunogenicity of the B subunit of the cholera toxin (CTB), a relevant immunomodulatory agent in vaccinology. An oligomeric CTB recombinant protein was expressed in corn smut galls at levels of up to 1.3 mg g-1 dry weight (0.8% of the total soluble protein). Mice orally immunized with ‘huitlacoche’-derived CTB showed significant humoral responses that were well-correlated with protection against challenge with the cholera toxin (CT). These findings demonstrate the feasibility of using edible corn smut as a safe, effective, and low-cost platform for production and delivery of a subunit oral vaccine. The implications of this platform in the area of molecular pharming are discussed. Public Library of Science 2015-07-24 /pmc/articles/PMC4514630/ /pubmed/26207365 http://dx.doi.org/10.1371/journal.pone.0133535 Text en © 2015 Juárez-Montiel et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Juárez-Montiel, Margarita
Romero-Maldonado, Andrea
Monreal-Escalante, Elizabeth
Becerra-Flora, Alicia
Korban, Schuyler S.
Rosales-Mendoza, Sergio
Jiménez-Bremont, Juan Francisco
The Corn Smut (‘Huitlacoche’) as a New Platform for Oral Vaccines
title The Corn Smut (‘Huitlacoche’) as a New Platform for Oral Vaccines
title_full The Corn Smut (‘Huitlacoche’) as a New Platform for Oral Vaccines
title_fullStr The Corn Smut (‘Huitlacoche’) as a New Platform for Oral Vaccines
title_full_unstemmed The Corn Smut (‘Huitlacoche’) as a New Platform for Oral Vaccines
title_short The Corn Smut (‘Huitlacoche’) as a New Platform for Oral Vaccines
title_sort corn smut (‘huitlacoche’) as a new platform for oral vaccines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514630/
https://www.ncbi.nlm.nih.gov/pubmed/26207365
http://dx.doi.org/10.1371/journal.pone.0133535
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