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DNA Microarray Characterization of Pathogens Associated with Sexually Transmitted Diseases

This study established a multiplex PCR-based microarray to detect simultaneously a diverse panel of 17 sexually transmitted diseases (STDs)-associated pathogens including Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma, Herpes simplex virus (HSV) t...

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Autores principales: Cao, Boyang, Wang, Suwei, Tian, Zhenyang, Hu, Pinliang, Feng, Lu, Wang, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514671/
https://www.ncbi.nlm.nih.gov/pubmed/26208181
http://dx.doi.org/10.1371/journal.pone.0133927
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author Cao, Boyang
Wang, Suwei
Tian, Zhenyang
Hu, Pinliang
Feng, Lu
Wang, Lei
author_facet Cao, Boyang
Wang, Suwei
Tian, Zhenyang
Hu, Pinliang
Feng, Lu
Wang, Lei
author_sort Cao, Boyang
collection PubMed
description This study established a multiplex PCR-based microarray to detect simultaneously a diverse panel of 17 sexually transmitted diseases (STDs)-associated pathogens including Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma, Herpes simplex virus (HSV) types 1 and 2, and Human papillomavirus (HPV) types 6, 11, 16, 18, 31, 33, 35, 39, 54 and 58. The target genes are 16S rRNA gene for N. gonorrhoeae, M. genitalium, M. hominism, and Ureaplasma, the major outer membrane protein gene (ompA) for C. trachomatis, the glycoprotein B gene (gB) for HSV; and the L1 gene for HPV. A total of 34 probes were selected for the microarray including 31 specific probes, one as positive control, one as negative control, and one as positional control probe for printing reference. The microarray is specific as the commensal and pathogenic microbes (and closely related organisms) in the genitourinary tract did not cross-react with the microarray probes. The microarray is 10 times more sensitive than that of the multiplex PCR. Among the 158 suspected HPV specimens examined, the microarray showed that 49 samples contained HPV, 21 samples contained Ureaplasma, 15 contained M. hominis, four contained C. trachomatis, and one contained N. gonorrhoeae. This work reports the development of the first high through-put detection system that identifies common pathogens associated with STDs from clinical samples, and paves the way for establishing a time-saving, accurate and high-throughput diagnostic tool for STDs.
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spelling pubmed-45146712015-07-29 DNA Microarray Characterization of Pathogens Associated with Sexually Transmitted Diseases Cao, Boyang Wang, Suwei Tian, Zhenyang Hu, Pinliang Feng, Lu Wang, Lei PLoS One Research Article This study established a multiplex PCR-based microarray to detect simultaneously a diverse panel of 17 sexually transmitted diseases (STDs)-associated pathogens including Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma, Herpes simplex virus (HSV) types 1 and 2, and Human papillomavirus (HPV) types 6, 11, 16, 18, 31, 33, 35, 39, 54 and 58. The target genes are 16S rRNA gene for N. gonorrhoeae, M. genitalium, M. hominism, and Ureaplasma, the major outer membrane protein gene (ompA) for C. trachomatis, the glycoprotein B gene (gB) for HSV; and the L1 gene for HPV. A total of 34 probes were selected for the microarray including 31 specific probes, one as positive control, one as negative control, and one as positional control probe for printing reference. The microarray is specific as the commensal and pathogenic microbes (and closely related organisms) in the genitourinary tract did not cross-react with the microarray probes. The microarray is 10 times more sensitive than that of the multiplex PCR. Among the 158 suspected HPV specimens examined, the microarray showed that 49 samples contained HPV, 21 samples contained Ureaplasma, 15 contained M. hominis, four contained C. trachomatis, and one contained N. gonorrhoeae. This work reports the development of the first high through-put detection system that identifies common pathogens associated with STDs from clinical samples, and paves the way for establishing a time-saving, accurate and high-throughput diagnostic tool for STDs. Public Library of Science 2015-07-24 /pmc/articles/PMC4514671/ /pubmed/26208181 http://dx.doi.org/10.1371/journal.pone.0133927 Text en © 2015 Cao et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Cao, Boyang
Wang, Suwei
Tian, Zhenyang
Hu, Pinliang
Feng, Lu
Wang, Lei
DNA Microarray Characterization of Pathogens Associated with Sexually Transmitted Diseases
title DNA Microarray Characterization of Pathogens Associated with Sexually Transmitted Diseases
title_full DNA Microarray Characterization of Pathogens Associated with Sexually Transmitted Diseases
title_fullStr DNA Microarray Characterization of Pathogens Associated with Sexually Transmitted Diseases
title_full_unstemmed DNA Microarray Characterization of Pathogens Associated with Sexually Transmitted Diseases
title_short DNA Microarray Characterization of Pathogens Associated with Sexually Transmitted Diseases
title_sort dna microarray characterization of pathogens associated with sexually transmitted diseases
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514671/
https://www.ncbi.nlm.nih.gov/pubmed/26208181
http://dx.doi.org/10.1371/journal.pone.0133927
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