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A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers

Serum and plasma calprotectin concentration is shown to be elevated when neutrophils are activated, and may therefore be used as a marker for inflammatory diseases. A serum calprotectin immunoassay was developed based on calprotectin values observed in samples from the intensive care unit. The polyc...

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Autores principales: Nilsen, Tom, Sunde, Kathrin, Larsson, Anders
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514941/
https://www.ncbi.nlm.nih.gov/pubmed/26213499
http://dx.doi.org/10.1186/s12950-015-0090-3
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author Nilsen, Tom
Sunde, Kathrin
Larsson, Anders
author_facet Nilsen, Tom
Sunde, Kathrin
Larsson, Anders
author_sort Nilsen, Tom
collection PubMed
description Serum and plasma calprotectin concentration is shown to be elevated when neutrophils are activated, and may therefore be used as a marker for inflammatory diseases. A serum calprotectin immunoassay was developed based on calprotectin values observed in samples from the intensive care unit. The polyclonal avian antibodies were raised and affinity purified with calprotectin antigens. The performance was tested and it was observed that the assay was linear in the range 0.3–24.7 mg/L, the limit of quantitation was observed to be lower than 0.3 mg/L, no antigen excess was observed up to 54 mg/L, all CVs were lower than 1.8 % in the precision study, the calibration curve stability was longer than 6 weeks, and there was no significant interference detected for haemoglobin, intralipid or bilirubin. The serum calprotectin immunoassay presented in this paper performs well within the criteria carefully set from the limited clinical experience obtained in both serum and plasma. In addition it is commutable with Bühlmann MRP8/14 ELISA.
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spelling pubmed-45149412015-07-26 A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers Nilsen, Tom Sunde, Kathrin Larsson, Anders J Inflamm (Lond) Research Serum and plasma calprotectin concentration is shown to be elevated when neutrophils are activated, and may therefore be used as a marker for inflammatory diseases. A serum calprotectin immunoassay was developed based on calprotectin values observed in samples from the intensive care unit. The polyclonal avian antibodies were raised and affinity purified with calprotectin antigens. The performance was tested and it was observed that the assay was linear in the range 0.3–24.7 mg/L, the limit of quantitation was observed to be lower than 0.3 mg/L, no antigen excess was observed up to 54 mg/L, all CVs were lower than 1.8 % in the precision study, the calibration curve stability was longer than 6 weeks, and there was no significant interference detected for haemoglobin, intralipid or bilirubin. The serum calprotectin immunoassay presented in this paper performs well within the criteria carefully set from the limited clinical experience obtained in both serum and plasma. In addition it is commutable with Bühlmann MRP8/14 ELISA. BioMed Central 2015-07-25 /pmc/articles/PMC4514941/ /pubmed/26213499 http://dx.doi.org/10.1186/s12950-015-0090-3 Text en © Nilsen et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Nilsen, Tom
Sunde, Kathrin
Larsson, Anders
A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers
title A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers
title_full A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers
title_fullStr A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers
title_full_unstemmed A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers
title_short A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers
title_sort new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4514941/
https://www.ncbi.nlm.nih.gov/pubmed/26213499
http://dx.doi.org/10.1186/s12950-015-0090-3
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