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Characterization of moose intestinal glycosphingolipids

As a part of a systematic investigation of the species-specific expression of glycosphingolipids, acid and non-acid glycosphingolipids were isolated from three small intestines and one large intestine of the moose (Alces alces). The glycosphingolipids were characterized by binding of monoclonal anti...

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Autores principales: Johansson, Miralda Madar, Dedic, Benjamin, Lundholm, Klara, Branzell, Filip Berner, Barone, Angela, Benktander, John, Teneberg, Susann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4515253/
https://www.ncbi.nlm.nih.gov/pubmed/26104834
http://dx.doi.org/10.1007/s10719-015-9604-8
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author Johansson, Miralda Madar
Dedic, Benjamin
Lundholm, Klara
Branzell, Filip Berner
Barone, Angela
Benktander, John
Teneberg, Susann
author_facet Johansson, Miralda Madar
Dedic, Benjamin
Lundholm, Klara
Branzell, Filip Berner
Barone, Angela
Benktander, John
Teneberg, Susann
author_sort Johansson, Miralda Madar
collection PubMed
description As a part of a systematic investigation of the species-specific expression of glycosphingolipids, acid and non-acid glycosphingolipids were isolated from three small intestines and one large intestine of the moose (Alces alces). The glycosphingolipids were characterized by binding of monoclonal antibodies, lectins and bacteria in chromatogram binding assays, and by mass spectrometry. The non-acid fractions were complex mixtures, and all had glycosphingolipids belonging to the lacto- and neolactoseries (lactotriaosylceramide, lactotetraosylceramide, neolactotetraosylceramide, Galα3-Le(x) hexaosylceramide, and lacto-neolactohexaosylceramide), globo-series (globotriaosylceramide and globotetraosylceramide), and isogloboseries (isoglobotriaosylceramide). Penta- and heptaglycosylceramides with terminal Galili determinants were also characterized. Furthermore, glycosphingolipids with terminal blood group O determinants (H triaosylceramide, H type 2 pentaosylceramide, H type 1 penta- and heptaosylceramide) were characterized in two of the moose small intestines, and in the one large intestine, while the third small intestine had glycosphingolipids with terminal blood group A determinants (A tetraosylceramide, A type 1 hexa- and octaosylceramide, A dodecaosylceramide). The acid glycosphingolipid fractions of moose small and large intestine contained sulfatide, and the gangliosides GM3, GD3, GD1a, GD1b, and also NeuGc and NeuAc variants of the Sd(a) ganglioside and the sialyl-globopenta/SSEA-4 ganglioside. In humans, the NeuAc-globopenta/SSEA-4 ganglioside is a marker of embryonic and adult stem cells, and is also expressed in several human cancers. This is the first time sialyl-globopentaosylceramide/SSEA-4 has been characterized in a fully differentiated normal tissue, and also the first time NeuGc-globopentaosylceramide has been characterized. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10719-015-9604-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-45152532015-07-27 Characterization of moose intestinal glycosphingolipids Johansson, Miralda Madar Dedic, Benjamin Lundholm, Klara Branzell, Filip Berner Barone, Angela Benktander, John Teneberg, Susann Glycoconj J Original Article As a part of a systematic investigation of the species-specific expression of glycosphingolipids, acid and non-acid glycosphingolipids were isolated from three small intestines and one large intestine of the moose (Alces alces). The glycosphingolipids were characterized by binding of monoclonal antibodies, lectins and bacteria in chromatogram binding assays, and by mass spectrometry. The non-acid fractions were complex mixtures, and all had glycosphingolipids belonging to the lacto- and neolactoseries (lactotriaosylceramide, lactotetraosylceramide, neolactotetraosylceramide, Galα3-Le(x) hexaosylceramide, and lacto-neolactohexaosylceramide), globo-series (globotriaosylceramide and globotetraosylceramide), and isogloboseries (isoglobotriaosylceramide). Penta- and heptaglycosylceramides with terminal Galili determinants were also characterized. Furthermore, glycosphingolipids with terminal blood group O determinants (H triaosylceramide, H type 2 pentaosylceramide, H type 1 penta- and heptaosylceramide) were characterized in two of the moose small intestines, and in the one large intestine, while the third small intestine had glycosphingolipids with terminal blood group A determinants (A tetraosylceramide, A type 1 hexa- and octaosylceramide, A dodecaosylceramide). The acid glycosphingolipid fractions of moose small and large intestine contained sulfatide, and the gangliosides GM3, GD3, GD1a, GD1b, and also NeuGc and NeuAc variants of the Sd(a) ganglioside and the sialyl-globopenta/SSEA-4 ganglioside. In humans, the NeuAc-globopenta/SSEA-4 ganglioside is a marker of embryonic and adult stem cells, and is also expressed in several human cancers. This is the first time sialyl-globopentaosylceramide/SSEA-4 has been characterized in a fully differentiated normal tissue, and also the first time NeuGc-globopentaosylceramide has been characterized. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10719-015-9604-8) contains supplementary material, which is available to authorized users. Springer US 2015-06-24 2015 /pmc/articles/PMC4515253/ /pubmed/26104834 http://dx.doi.org/10.1007/s10719-015-9604-8 Text en © The Author(s) 2015 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Johansson, Miralda Madar
Dedic, Benjamin
Lundholm, Klara
Branzell, Filip Berner
Barone, Angela
Benktander, John
Teneberg, Susann
Characterization of moose intestinal glycosphingolipids
title Characterization of moose intestinal glycosphingolipids
title_full Characterization of moose intestinal glycosphingolipids
title_fullStr Characterization of moose intestinal glycosphingolipids
title_full_unstemmed Characterization of moose intestinal glycosphingolipids
title_short Characterization of moose intestinal glycosphingolipids
title_sort characterization of moose intestinal glycosphingolipids
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4515253/
https://www.ncbi.nlm.nih.gov/pubmed/26104834
http://dx.doi.org/10.1007/s10719-015-9604-8
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