Laser-scanning cytometry can quantify human adipocyte browning and proves effectiveness of irisin

Laser-scanning cytometry is presented as a tool allowing population scale analysis of ex vivo human brown adipogenic differentiation. It combines texture analysis and detection of Ucp1 protein content in single brown adipocytes of mixed cell populations with gene expression pattern and functional ch...

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Detalles Bibliográficos
Autores principales: Kristóf, Endre, Doan-Xuan, Quang-Minh, Bai, Péter, Bacso, Zsolt, Fésüs, László
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4515591/
https://www.ncbi.nlm.nih.gov/pubmed/26212086
http://dx.doi.org/10.1038/srep12540
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author Kristóf, Endre
Doan-Xuan, Quang-Minh
Bai, Péter
Bacso, Zsolt
Fésüs, László
author_facet Kristóf, Endre
Doan-Xuan, Quang-Minh
Bai, Péter
Bacso, Zsolt
Fésüs, László
author_sort Kristóf, Endre
collection PubMed
description Laser-scanning cytometry is presented as a tool allowing population scale analysis of ex vivo human brown adipogenic differentiation. It combines texture analysis and detection of Ucp1 protein content in single brown adipocytes of mixed cell populations with gene expression pattern and functional characteristics of browning. Using this method we could validate mouse data in human samples demonstrating the effectiveness of irisin to induce “beige” differentiation of subcutaneous white adipocytes.
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spelling pubmed-45155912015-07-29 Laser-scanning cytometry can quantify human adipocyte browning and proves effectiveness of irisin Kristóf, Endre Doan-Xuan, Quang-Minh Bai, Péter Bacso, Zsolt Fésüs, László Sci Rep Article Laser-scanning cytometry is presented as a tool allowing population scale analysis of ex vivo human brown adipogenic differentiation. It combines texture analysis and detection of Ucp1 protein content in single brown adipocytes of mixed cell populations with gene expression pattern and functional characteristics of browning. Using this method we could validate mouse data in human samples demonstrating the effectiveness of irisin to induce “beige” differentiation of subcutaneous white adipocytes. Nature Publishing Group 2015-07-27 /pmc/articles/PMC4515591/ /pubmed/26212086 http://dx.doi.org/10.1038/srep12540 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Kristóf, Endre
Doan-Xuan, Quang-Minh
Bai, Péter
Bacso, Zsolt
Fésüs, László
Laser-scanning cytometry can quantify human adipocyte browning and proves effectiveness of irisin
title Laser-scanning cytometry can quantify human adipocyte browning and proves effectiveness of irisin
title_full Laser-scanning cytometry can quantify human adipocyte browning and proves effectiveness of irisin
title_fullStr Laser-scanning cytometry can quantify human adipocyte browning and proves effectiveness of irisin
title_full_unstemmed Laser-scanning cytometry can quantify human adipocyte browning and proves effectiveness of irisin
title_short Laser-scanning cytometry can quantify human adipocyte browning and proves effectiveness of irisin
title_sort laser-scanning cytometry can quantify human adipocyte browning and proves effectiveness of irisin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4515591/
https://www.ncbi.nlm.nih.gov/pubmed/26212086
http://dx.doi.org/10.1038/srep12540
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