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Functional and Structural Characterization of FAU Gene/Protein from Marine Sponge Suberites domuncula

Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed (FAU) gene is down-regulated in human prostate, breast and ovarian cancers. Moreover, its dysregulation is associated with poor prognosis in breast cancer. Sponges (Porifera) are animals without tissues which branched off fi...

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Autores principales: Perina, Dragutin, Korolija, Marina, Popović Hadžija, Marijana, Grbeša, Ivana, Belužić, Robert, Imešek, Mirna, Morrow, Christine, Marjanović, Melanija Posavec, Bakran-Petricioli, Tatjana, Mikoč, Andreja, Ćetković, Helena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4515611/
https://www.ncbi.nlm.nih.gov/pubmed/26198235
http://dx.doi.org/10.3390/md13074179
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author Perina, Dragutin
Korolija, Marina
Popović Hadžija, Marijana
Grbeša, Ivana
Belužić, Robert
Imešek, Mirna
Morrow, Christine
Marjanović, Melanija Posavec
Bakran-Petricioli, Tatjana
Mikoč, Andreja
Ćetković, Helena
author_facet Perina, Dragutin
Korolija, Marina
Popović Hadžija, Marijana
Grbeša, Ivana
Belužić, Robert
Imešek, Mirna
Morrow, Christine
Marjanović, Melanija Posavec
Bakran-Petricioli, Tatjana
Mikoč, Andreja
Ćetković, Helena
author_sort Perina, Dragutin
collection PubMed
description Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed (FAU) gene is down-regulated in human prostate, breast and ovarian cancers. Moreover, its dysregulation is associated with poor prognosis in breast cancer. Sponges (Porifera) are animals without tissues which branched off first from the common ancestor of all metazoans. A large majority of genes implicated in human cancers have their homologues in the sponge genome. Our study suggests that FAU gene from the sponge Suberites domuncula reflects characteristics of the FAU gene from the metazoan ancestor, which have changed only slightly during the course of animal evolution. We found pro-apoptotic activity of sponge FAU protein. The same as its human homologue, sponge FAU increases apoptosis in human HEK293T cells. This indicates that the biological functions of FAU, usually associated with “higher” metazoans, particularly in cancer etiology, possess a biochemical background established early in metazoan evolution. The ancestor of all animals possibly possessed FAU protein with the structure and function similar to evolutionarily more recent versions of the protein, even before the appearance of true tissues and the origin of tumors and metastasis. It provides an opportunity to use pre-bilaterian animals as a simpler model for studying complex interactions in human cancerogenesis.
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spelling pubmed-45156112015-07-28 Functional and Structural Characterization of FAU Gene/Protein from Marine Sponge Suberites domuncula Perina, Dragutin Korolija, Marina Popović Hadžija, Marijana Grbeša, Ivana Belužić, Robert Imešek, Mirna Morrow, Christine Marjanović, Melanija Posavec Bakran-Petricioli, Tatjana Mikoč, Andreja Ćetković, Helena Mar Drugs Article Finkel-Biskis-Reilly murine sarcoma virus (FBR-MuSV) ubiquitously expressed (FAU) gene is down-regulated in human prostate, breast and ovarian cancers. Moreover, its dysregulation is associated with poor prognosis in breast cancer. Sponges (Porifera) are animals without tissues which branched off first from the common ancestor of all metazoans. A large majority of genes implicated in human cancers have their homologues in the sponge genome. Our study suggests that FAU gene from the sponge Suberites domuncula reflects characteristics of the FAU gene from the metazoan ancestor, which have changed only slightly during the course of animal evolution. We found pro-apoptotic activity of sponge FAU protein. The same as its human homologue, sponge FAU increases apoptosis in human HEK293T cells. This indicates that the biological functions of FAU, usually associated with “higher” metazoans, particularly in cancer etiology, possess a biochemical background established early in metazoan evolution. The ancestor of all animals possibly possessed FAU protein with the structure and function similar to evolutionarily more recent versions of the protein, even before the appearance of true tissues and the origin of tumors and metastasis. It provides an opportunity to use pre-bilaterian animals as a simpler model for studying complex interactions in human cancerogenesis. MDPI 2015-07-07 /pmc/articles/PMC4515611/ /pubmed/26198235 http://dx.doi.org/10.3390/md13074179 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Perina, Dragutin
Korolija, Marina
Popović Hadžija, Marijana
Grbeša, Ivana
Belužić, Robert
Imešek, Mirna
Morrow, Christine
Marjanović, Melanija Posavec
Bakran-Petricioli, Tatjana
Mikoč, Andreja
Ćetković, Helena
Functional and Structural Characterization of FAU Gene/Protein from Marine Sponge Suberites domuncula
title Functional and Structural Characterization of FAU Gene/Protein from Marine Sponge Suberites domuncula
title_full Functional and Structural Characterization of FAU Gene/Protein from Marine Sponge Suberites domuncula
title_fullStr Functional and Structural Characterization of FAU Gene/Protein from Marine Sponge Suberites domuncula
title_full_unstemmed Functional and Structural Characterization of FAU Gene/Protein from Marine Sponge Suberites domuncula
title_short Functional and Structural Characterization of FAU Gene/Protein from Marine Sponge Suberites domuncula
title_sort functional and structural characterization of fau gene/protein from marine sponge suberites domuncula
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4515611/
https://www.ncbi.nlm.nih.gov/pubmed/26198235
http://dx.doi.org/10.3390/md13074179
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