Cargando…
Knockout of SRC-1 and SRC-3 in Mice Decreases Cardiomyocyte Proliferation and Causes a Noncompaction Cardiomyopathy Phenotype
Noncompaction cardiomyopathy (NCC) is a congenital heart disease that causes ventricular dysfunction and high mortality rate in children. The mechanisms responsible for NCC are still unknown. The steroid receptor coactivator-1 (SRC-1) and SRC-3 are transcriptional coactivators for nuclear hormone re...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4515817/ https://www.ncbi.nlm.nih.gov/pubmed/26221073 http://dx.doi.org/10.7150/ijbs.12408 |
_version_ | 1782382976517013504 |
---|---|
author | Chen, Xian Qin, Li Liu, Zhaoliang Liao, Lan Martin, James F. Xu, Jianming |
author_facet | Chen, Xian Qin, Li Liu, Zhaoliang Liao, Lan Martin, James F. Xu, Jianming |
author_sort | Chen, Xian |
collection | PubMed |
description | Noncompaction cardiomyopathy (NCC) is a congenital heart disease that causes ventricular dysfunction and high mortality rate in children. The mechanisms responsible for NCC are still unknown. The steroid receptor coactivator-1 (SRC-1) and SRC-3 are transcriptional coactivators for nuclear hormone receptors and certain other transcription factors that regulate many genes in development and organ function. However, the roles of SRC-1/3 in heart morphogenesis, function and NCC occurrence are unknown. This study aims to examine the spatial and temporal expression patterns of SRC-1/3 in the heart and investigate the specific roles of SRC-1/3 in heart development, function and NCC occurrence. Immunochemical analysis detected SRC-1/3 expressions in the proliferating cardiomyocytes of mouse heart at prenatal and neonatal stages, while these expressions disappeared within two weeks after birth. Through generating and characterizing mouse lines with global or cardiomyocyte-specific knockouts of SRC-1/3, we found ablation of SRC-1/3 in the myocardial lineage resulted in prominent trabeculae, deep intertrabecular recesses and thin ventricular wall and septum. These developmental defects caused a failure of trabecular compaction, decreased internal ventricular dimension, reduced cardiac ejection fraction and output and led to a high rate of postnatal mortality. Collectively, these structural and functional abnormalities closely simulate the phenotype of NCC patients. Further molecular analysis of cardiomyocytes in vivo and in vitro revealed that SRC-1/3 directly up-regulate cyclin E2, cyclin B1 and myocardin to promote cardiomyocyte proliferation and differentiation. In conclusion, SRC-1/3 are required for cardiomyocyte proliferation and differentiation at earlier developmental stages, and their dysfunction causes NCC-like abnormalities in the hearts of newborn and adult mice. |
format | Online Article Text |
id | pubmed-4515817 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-45158172015-07-28 Knockout of SRC-1 and SRC-3 in Mice Decreases Cardiomyocyte Proliferation and Causes a Noncompaction Cardiomyopathy Phenotype Chen, Xian Qin, Li Liu, Zhaoliang Liao, Lan Martin, James F. Xu, Jianming Int J Biol Sci Research Paper Noncompaction cardiomyopathy (NCC) is a congenital heart disease that causes ventricular dysfunction and high mortality rate in children. The mechanisms responsible for NCC are still unknown. The steroid receptor coactivator-1 (SRC-1) and SRC-3 are transcriptional coactivators for nuclear hormone receptors and certain other transcription factors that regulate many genes in development and organ function. However, the roles of SRC-1/3 in heart morphogenesis, function and NCC occurrence are unknown. This study aims to examine the spatial and temporal expression patterns of SRC-1/3 in the heart and investigate the specific roles of SRC-1/3 in heart development, function and NCC occurrence. Immunochemical analysis detected SRC-1/3 expressions in the proliferating cardiomyocytes of mouse heart at prenatal and neonatal stages, while these expressions disappeared within two weeks after birth. Through generating and characterizing mouse lines with global or cardiomyocyte-specific knockouts of SRC-1/3, we found ablation of SRC-1/3 in the myocardial lineage resulted in prominent trabeculae, deep intertrabecular recesses and thin ventricular wall and septum. These developmental defects caused a failure of trabecular compaction, decreased internal ventricular dimension, reduced cardiac ejection fraction and output and led to a high rate of postnatal mortality. Collectively, these structural and functional abnormalities closely simulate the phenotype of NCC patients. Further molecular analysis of cardiomyocytes in vivo and in vitro revealed that SRC-1/3 directly up-regulate cyclin E2, cyclin B1 and myocardin to promote cardiomyocyte proliferation and differentiation. In conclusion, SRC-1/3 are required for cardiomyocyte proliferation and differentiation at earlier developmental stages, and their dysfunction causes NCC-like abnormalities in the hearts of newborn and adult mice. Ivyspring International Publisher 2015-07-15 /pmc/articles/PMC4515817/ /pubmed/26221073 http://dx.doi.org/10.7150/ijbs.12408 Text en © 2015 Ivyspring International Publisher. Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. See http://ivyspring.com/terms for terms and conditions. |
spellingShingle | Research Paper Chen, Xian Qin, Li Liu, Zhaoliang Liao, Lan Martin, James F. Xu, Jianming Knockout of SRC-1 and SRC-3 in Mice Decreases Cardiomyocyte Proliferation and Causes a Noncompaction Cardiomyopathy Phenotype |
title | Knockout of SRC-1 and SRC-3 in Mice Decreases Cardiomyocyte Proliferation and Causes a Noncompaction Cardiomyopathy Phenotype |
title_full | Knockout of SRC-1 and SRC-3 in Mice Decreases Cardiomyocyte Proliferation and Causes a Noncompaction Cardiomyopathy Phenotype |
title_fullStr | Knockout of SRC-1 and SRC-3 in Mice Decreases Cardiomyocyte Proliferation and Causes a Noncompaction Cardiomyopathy Phenotype |
title_full_unstemmed | Knockout of SRC-1 and SRC-3 in Mice Decreases Cardiomyocyte Proliferation and Causes a Noncompaction Cardiomyopathy Phenotype |
title_short | Knockout of SRC-1 and SRC-3 in Mice Decreases Cardiomyocyte Proliferation and Causes a Noncompaction Cardiomyopathy Phenotype |
title_sort | knockout of src-1 and src-3 in mice decreases cardiomyocyte proliferation and causes a noncompaction cardiomyopathy phenotype |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4515817/ https://www.ncbi.nlm.nih.gov/pubmed/26221073 http://dx.doi.org/10.7150/ijbs.12408 |
work_keys_str_mv | AT chenxian knockoutofsrc1andsrc3inmicedecreasescardiomyocyteproliferationandcausesanoncompactioncardiomyopathyphenotype AT qinli knockoutofsrc1andsrc3inmicedecreasescardiomyocyteproliferationandcausesanoncompactioncardiomyopathyphenotype AT liuzhaoliang knockoutofsrc1andsrc3inmicedecreasescardiomyocyteproliferationandcausesanoncompactioncardiomyopathyphenotype AT liaolan knockoutofsrc1andsrc3inmicedecreasescardiomyocyteproliferationandcausesanoncompactioncardiomyopathyphenotype AT martinjamesf knockoutofsrc1andsrc3inmicedecreasescardiomyocyteproliferationandcausesanoncompactioncardiomyopathyphenotype AT xujianming knockoutofsrc1andsrc3inmicedecreasescardiomyocyteproliferationandcausesanoncompactioncardiomyopathyphenotype |