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Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface
To target chronic inflammatory ocular surface diseases, a drug delivery platform is needed that is safe, possesses immunomodulatory properties, and can be used either for drug delivery, or as a foreign antigen carrier. A new therapeutic approach that we have previously proposed uses nonliving bacter...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4516183/ https://www.ncbi.nlm.nih.gov/pubmed/26229437 http://dx.doi.org/10.2147/DDDT.S84370 |
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author | Montanaro, Jacqueline Inic-Kanada, Aleksandra Ladurner, Angela Stein, Elisabeth Belij, Sandra Bintner, Nora Schlacher, Simone Schuerer, Nadine Mayr, Ulrike Beate Lubitz, Werner Leisch, Nikolaus Barisani-Asenbauer, Talin |
author_facet | Montanaro, Jacqueline Inic-Kanada, Aleksandra Ladurner, Angela Stein, Elisabeth Belij, Sandra Bintner, Nora Schlacher, Simone Schuerer, Nadine Mayr, Ulrike Beate Lubitz, Werner Leisch, Nikolaus Barisani-Asenbauer, Talin |
author_sort | Montanaro, Jacqueline |
collection | PubMed |
description | To target chronic inflammatory ocular surface diseases, a drug delivery platform is needed that is safe, possesses immunomodulatory properties, and can be used either for drug delivery, or as a foreign antigen carrier. A new therapeutic approach that we have previously proposed uses nonliving bacterial ghosts (BGs) as a carrier-delivery system which can be engineered to carry foreign antigens and/or be loaded with therapeutic drugs. The parent strain chosen for development of our BG delivery system is the probiotic Escherichia coli strain Nissle 1917 (EcN), whose intrinsic properties trigger the innate immune system with the flagella and fimbriae used to attach and stimulate epithelial cells. In previous studies, we have shown that EcN BGs are safe for the ocular surface route, but evidence that EcN BGs retain flagella and fimbriae after transformation, has never been visually confirmed. In this study, we used different visualization techniques to determine whether flagella and fimbriae are retained on EcN BGs engineered either for drug delivery or as a foreign antigen carrier. We have also shown by immunoelectron microscopy that EcN retains two foreign antigens after processing to become EcN BGs. Furthermore, we demonstrated that BGs derived from EcN and expressing a foreign antigen attachment to conjunctival epithelial cells in vitro without causing reduced cell viability. These results are an important step in constructing a delivery system based on a nonliving probiotic that is suitable for use in ocular surface diseases pairing immunomodulation and targeted delivery. |
format | Online Article Text |
id | pubmed-4516183 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-45161832015-07-30 Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface Montanaro, Jacqueline Inic-Kanada, Aleksandra Ladurner, Angela Stein, Elisabeth Belij, Sandra Bintner, Nora Schlacher, Simone Schuerer, Nadine Mayr, Ulrike Beate Lubitz, Werner Leisch, Nikolaus Barisani-Asenbauer, Talin Drug Des Devel Ther Original Research To target chronic inflammatory ocular surface diseases, a drug delivery platform is needed that is safe, possesses immunomodulatory properties, and can be used either for drug delivery, or as a foreign antigen carrier. A new therapeutic approach that we have previously proposed uses nonliving bacterial ghosts (BGs) as a carrier-delivery system which can be engineered to carry foreign antigens and/or be loaded with therapeutic drugs. The parent strain chosen for development of our BG delivery system is the probiotic Escherichia coli strain Nissle 1917 (EcN), whose intrinsic properties trigger the innate immune system with the flagella and fimbriae used to attach and stimulate epithelial cells. In previous studies, we have shown that EcN BGs are safe for the ocular surface route, but evidence that EcN BGs retain flagella and fimbriae after transformation, has never been visually confirmed. In this study, we used different visualization techniques to determine whether flagella and fimbriae are retained on EcN BGs engineered either for drug delivery or as a foreign antigen carrier. We have also shown by immunoelectron microscopy that EcN retains two foreign antigens after processing to become EcN BGs. Furthermore, we demonstrated that BGs derived from EcN and expressing a foreign antigen attachment to conjunctival epithelial cells in vitro without causing reduced cell viability. These results are an important step in constructing a delivery system based on a nonliving probiotic that is suitable for use in ocular surface diseases pairing immunomodulation and targeted delivery. Dove Medical Press 2015-07-21 /pmc/articles/PMC4516183/ /pubmed/26229437 http://dx.doi.org/10.2147/DDDT.S84370 Text en © 2015 Montanaro et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Montanaro, Jacqueline Inic-Kanada, Aleksandra Ladurner, Angela Stein, Elisabeth Belij, Sandra Bintner, Nora Schlacher, Simone Schuerer, Nadine Mayr, Ulrike Beate Lubitz, Werner Leisch, Nikolaus Barisani-Asenbauer, Talin Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface |
title | Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface |
title_full | Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface |
title_fullStr | Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface |
title_full_unstemmed | Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface |
title_short | Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface |
title_sort | escherichia coli nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4516183/ https://www.ncbi.nlm.nih.gov/pubmed/26229437 http://dx.doi.org/10.2147/DDDT.S84370 |
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