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Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface

To target chronic inflammatory ocular surface diseases, a drug delivery platform is needed that is safe, possesses immunomodulatory properties, and can be used either for drug delivery, or as a foreign antigen carrier. A new therapeutic approach that we have previously proposed uses nonliving bacter...

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Autores principales: Montanaro, Jacqueline, Inic-Kanada, Aleksandra, Ladurner, Angela, Stein, Elisabeth, Belij, Sandra, Bintner, Nora, Schlacher, Simone, Schuerer, Nadine, Mayr, Ulrike Beate, Lubitz, Werner, Leisch, Nikolaus, Barisani-Asenbauer, Talin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4516183/
https://www.ncbi.nlm.nih.gov/pubmed/26229437
http://dx.doi.org/10.2147/DDDT.S84370
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author Montanaro, Jacqueline
Inic-Kanada, Aleksandra
Ladurner, Angela
Stein, Elisabeth
Belij, Sandra
Bintner, Nora
Schlacher, Simone
Schuerer, Nadine
Mayr, Ulrike Beate
Lubitz, Werner
Leisch, Nikolaus
Barisani-Asenbauer, Talin
author_facet Montanaro, Jacqueline
Inic-Kanada, Aleksandra
Ladurner, Angela
Stein, Elisabeth
Belij, Sandra
Bintner, Nora
Schlacher, Simone
Schuerer, Nadine
Mayr, Ulrike Beate
Lubitz, Werner
Leisch, Nikolaus
Barisani-Asenbauer, Talin
author_sort Montanaro, Jacqueline
collection PubMed
description To target chronic inflammatory ocular surface diseases, a drug delivery platform is needed that is safe, possesses immunomodulatory properties, and can be used either for drug delivery, or as a foreign antigen carrier. A new therapeutic approach that we have previously proposed uses nonliving bacterial ghosts (BGs) as a carrier-delivery system which can be engineered to carry foreign antigens and/or be loaded with therapeutic drugs. The parent strain chosen for development of our BG delivery system is the probiotic Escherichia coli strain Nissle 1917 (EcN), whose intrinsic properties trigger the innate immune system with the flagella and fimbriae used to attach and stimulate epithelial cells. In previous studies, we have shown that EcN BGs are safe for the ocular surface route, but evidence that EcN BGs retain flagella and fimbriae after transformation, has never been visually confirmed. In this study, we used different visualization techniques to determine whether flagella and fimbriae are retained on EcN BGs engineered either for drug delivery or as a foreign antigen carrier. We have also shown by immunoelectron microscopy that EcN retains two foreign antigens after processing to become EcN BGs. Furthermore, we demonstrated that BGs derived from EcN and expressing a foreign antigen attachment to conjunctival epithelial cells in vitro without causing reduced cell viability. These results are an important step in constructing a delivery system based on a nonliving probiotic that is suitable for use in ocular surface diseases pairing immunomodulation and targeted delivery.
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spelling pubmed-45161832015-07-30 Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface Montanaro, Jacqueline Inic-Kanada, Aleksandra Ladurner, Angela Stein, Elisabeth Belij, Sandra Bintner, Nora Schlacher, Simone Schuerer, Nadine Mayr, Ulrike Beate Lubitz, Werner Leisch, Nikolaus Barisani-Asenbauer, Talin Drug Des Devel Ther Original Research To target chronic inflammatory ocular surface diseases, a drug delivery platform is needed that is safe, possesses immunomodulatory properties, and can be used either for drug delivery, or as a foreign antigen carrier. A new therapeutic approach that we have previously proposed uses nonliving bacterial ghosts (BGs) as a carrier-delivery system which can be engineered to carry foreign antigens and/or be loaded with therapeutic drugs. The parent strain chosen for development of our BG delivery system is the probiotic Escherichia coli strain Nissle 1917 (EcN), whose intrinsic properties trigger the innate immune system with the flagella and fimbriae used to attach and stimulate epithelial cells. In previous studies, we have shown that EcN BGs are safe for the ocular surface route, but evidence that EcN BGs retain flagella and fimbriae after transformation, has never been visually confirmed. In this study, we used different visualization techniques to determine whether flagella and fimbriae are retained on EcN BGs engineered either for drug delivery or as a foreign antigen carrier. We have also shown by immunoelectron microscopy that EcN retains two foreign antigens after processing to become EcN BGs. Furthermore, we demonstrated that BGs derived from EcN and expressing a foreign antigen attachment to conjunctival epithelial cells in vitro without causing reduced cell viability. These results are an important step in constructing a delivery system based on a nonliving probiotic that is suitable for use in ocular surface diseases pairing immunomodulation and targeted delivery. Dove Medical Press 2015-07-21 /pmc/articles/PMC4516183/ /pubmed/26229437 http://dx.doi.org/10.2147/DDDT.S84370 Text en © 2015 Montanaro et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Montanaro, Jacqueline
Inic-Kanada, Aleksandra
Ladurner, Angela
Stein, Elisabeth
Belij, Sandra
Bintner, Nora
Schlacher, Simone
Schuerer, Nadine
Mayr, Ulrike Beate
Lubitz, Werner
Leisch, Nikolaus
Barisani-Asenbauer, Talin
Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface
title Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface
title_full Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface
title_fullStr Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface
title_full_unstemmed Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface
title_short Escherichia coli Nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface
title_sort escherichia coli nissle 1917 bacterial ghosts retain crucial surface properties and express chlamydial antigen: an imaging study of a delivery system for the ocular surface
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4516183/
https://www.ncbi.nlm.nih.gov/pubmed/26229437
http://dx.doi.org/10.2147/DDDT.S84370
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