Cargando…
Identification of Cryptosporidium Species in Fish from Lake Geneva (Lac Léman) in France
Cryptosporidium, a protozoan parasite that can cause severe diarrhea in a wide range of vertebrates including humans, is increasingly recognized as a parasite of a diverse range of wildlife species. However, little data are available regarding the identification of Cryptosporidium species and genoty...
Autores principales: | , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4516323/ https://www.ncbi.nlm.nih.gov/pubmed/26213992 http://dx.doi.org/10.1371/journal.pone.0133047 |
_version_ | 1782383045706252288 |
---|---|
author | Certad, Gabriela Dupouy-Camet, Jean Gantois, Nausicaa Hammouma-Ghelboun, Ourida Pottier, Muriel Guyot, Karine Benamrouz, Sadia Osman, Marwan Delaire, Baptiste Creusy, Colette Viscogliosi, Eric Dei-Cas, Eduardo Aliouat-Denis, Cecile Marie Follet, Jérôme |
author_facet | Certad, Gabriela Dupouy-Camet, Jean Gantois, Nausicaa Hammouma-Ghelboun, Ourida Pottier, Muriel Guyot, Karine Benamrouz, Sadia Osman, Marwan Delaire, Baptiste Creusy, Colette Viscogliosi, Eric Dei-Cas, Eduardo Aliouat-Denis, Cecile Marie Follet, Jérôme |
author_sort | Certad, Gabriela |
collection | PubMed |
description | Cryptosporidium, a protozoan parasite that can cause severe diarrhea in a wide range of vertebrates including humans, is increasingly recognized as a parasite of a diverse range of wildlife species. However, little data are available regarding the identification of Cryptosporidium species and genotypes in wild aquatic environments, and more particularly in edible freshwater fish. To evaluate the prevalence of Cryptosporidiumspp. in fish from Lake Geneva (Lac Léman) in France, 41 entire fish and 100 fillets (cuts of fish flesh) were collected from fishery suppliers around the lake. Nested PCR using degenerate primers followed by sequence analysis was used. Five fish species were identified as potential hosts of Cryptosporidium: Salvelinus alpinus, Esox lucius, Coregonus lavaretus, Perca fluviatilis, and Rutilus rutilus. The presence of Cryptosporidium spp. was found in 15 out of 41 fish (37%), distributed as follows: 13 (87%) C. parvum, 1 (7%) C. molnari, and 1 (7%) mixed infection (C. parvum and C. molnari). C. molnari was identified in the stomach, while C. parvum was found in the stomach and intestine. C. molnari was also detected in 1 out of 100 analyzed fillets. In order to identify Cryptosporidium subtypes, sequencing of the highly polymorphic 60-kDa glycoprotein (gp60) was performed. Among the C. parvum positive samples, three gp60 subtypes were identified: IIaA15G2R1, IIaA16G2R1, and IIaA17G2R1. Histological examination confirmed the presence of potential developmental stages of C. parvum within digestive epithelial cells. These observations suggest that C. parvum is infecting fish, rather than being passively carried. Since C. parvum is a zoonotic species, fish potentially contaminated by the same subtypes found in terrestrial mammals would be an additional source of infection for humans and animals, and may also contribute to the contamination of the environment with this parasite. Moreover, the risk of human transmission is strengthened by the observation of edible fillet contamination. |
format | Online Article Text |
id | pubmed-4516323 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-45163232015-07-29 Identification of Cryptosporidium Species in Fish from Lake Geneva (Lac Léman) in France Certad, Gabriela Dupouy-Camet, Jean Gantois, Nausicaa Hammouma-Ghelboun, Ourida Pottier, Muriel Guyot, Karine Benamrouz, Sadia Osman, Marwan Delaire, Baptiste Creusy, Colette Viscogliosi, Eric Dei-Cas, Eduardo Aliouat-Denis, Cecile Marie Follet, Jérôme PLoS One Research Article Cryptosporidium, a protozoan parasite that can cause severe diarrhea in a wide range of vertebrates including humans, is increasingly recognized as a parasite of a diverse range of wildlife species. However, little data are available regarding the identification of Cryptosporidium species and genotypes in wild aquatic environments, and more particularly in edible freshwater fish. To evaluate the prevalence of Cryptosporidiumspp. in fish from Lake Geneva (Lac Léman) in France, 41 entire fish and 100 fillets (cuts of fish flesh) were collected from fishery suppliers around the lake. Nested PCR using degenerate primers followed by sequence analysis was used. Five fish species were identified as potential hosts of Cryptosporidium: Salvelinus alpinus, Esox lucius, Coregonus lavaretus, Perca fluviatilis, and Rutilus rutilus. The presence of Cryptosporidium spp. was found in 15 out of 41 fish (37%), distributed as follows: 13 (87%) C. parvum, 1 (7%) C. molnari, and 1 (7%) mixed infection (C. parvum and C. molnari). C. molnari was identified in the stomach, while C. parvum was found in the stomach and intestine. C. molnari was also detected in 1 out of 100 analyzed fillets. In order to identify Cryptosporidium subtypes, sequencing of the highly polymorphic 60-kDa glycoprotein (gp60) was performed. Among the C. parvum positive samples, three gp60 subtypes were identified: IIaA15G2R1, IIaA16G2R1, and IIaA17G2R1. Histological examination confirmed the presence of potential developmental stages of C. parvum within digestive epithelial cells. These observations suggest that C. parvum is infecting fish, rather than being passively carried. Since C. parvum is a zoonotic species, fish potentially contaminated by the same subtypes found in terrestrial mammals would be an additional source of infection for humans and animals, and may also contribute to the contamination of the environment with this parasite. Moreover, the risk of human transmission is strengthened by the observation of edible fillet contamination. Public Library of Science 2015-07-27 /pmc/articles/PMC4516323/ /pubmed/26213992 http://dx.doi.org/10.1371/journal.pone.0133047 Text en © 2015 Certad et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Certad, Gabriela Dupouy-Camet, Jean Gantois, Nausicaa Hammouma-Ghelboun, Ourida Pottier, Muriel Guyot, Karine Benamrouz, Sadia Osman, Marwan Delaire, Baptiste Creusy, Colette Viscogliosi, Eric Dei-Cas, Eduardo Aliouat-Denis, Cecile Marie Follet, Jérôme Identification of Cryptosporidium Species in Fish from Lake Geneva (Lac Léman) in France |
title | Identification of Cryptosporidium Species in Fish from Lake Geneva (Lac Léman) in France |
title_full | Identification of Cryptosporidium Species in Fish from Lake Geneva (Lac Léman) in France |
title_fullStr | Identification of Cryptosporidium Species in Fish from Lake Geneva (Lac Léman) in France |
title_full_unstemmed | Identification of Cryptosporidium Species in Fish from Lake Geneva (Lac Léman) in France |
title_short | Identification of Cryptosporidium Species in Fish from Lake Geneva (Lac Léman) in France |
title_sort | identification of cryptosporidium species in fish from lake geneva (lac léman) in france |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4516323/ https://www.ncbi.nlm.nih.gov/pubmed/26213992 http://dx.doi.org/10.1371/journal.pone.0133047 |
work_keys_str_mv | AT certadgabriela identificationofcryptosporidiumspeciesinfishfromlakegenevalaclemaninfrance AT dupouycametjean identificationofcryptosporidiumspeciesinfishfromlakegenevalaclemaninfrance AT gantoisnausicaa identificationofcryptosporidiumspeciesinfishfromlakegenevalaclemaninfrance AT hammoumaghelbounourida identificationofcryptosporidiumspeciesinfishfromlakegenevalaclemaninfrance AT pottiermuriel identificationofcryptosporidiumspeciesinfishfromlakegenevalaclemaninfrance AT guyotkarine identificationofcryptosporidiumspeciesinfishfromlakegenevalaclemaninfrance AT benamrouzsadia identificationofcryptosporidiumspeciesinfishfromlakegenevalaclemaninfrance AT osmanmarwan identificationofcryptosporidiumspeciesinfishfromlakegenevalaclemaninfrance AT delairebaptiste identificationofcryptosporidiumspeciesinfishfromlakegenevalaclemaninfrance AT creusycolette identificationofcryptosporidiumspeciesinfishfromlakegenevalaclemaninfrance AT viscogliosieric identificationofcryptosporidiumspeciesinfishfromlakegenevalaclemaninfrance AT deicaseduardo identificationofcryptosporidiumspeciesinfishfromlakegenevalaclemaninfrance AT aliouatdeniscecilemarie identificationofcryptosporidiumspeciesinfishfromlakegenevalaclemaninfrance AT folletjerome identificationofcryptosporidiumspeciesinfishfromlakegenevalaclemaninfrance |