Cargando…
Cyclooxygenase in normal human tissues – is COX-1 really a constitutive isoform, and COX-2 an inducible isoform?
Cyclooxygenase (COX) is a key enzyme in prostanoid synthesis. It exists in two isoforms, COX-1 and COX-2. COX-1 is referred to as a ‘constitutive isoform’, and is considered to be expressed in most tissues under basal conditions. In contrast, COX-2 is referred to as an ‘inducible isoform’, which is...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Ltd
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4516524/ https://www.ncbi.nlm.nih.gov/pubmed/18657230 http://dx.doi.org/10.1111/j.1582-4934.2008.00430.x |
_version_ | 1782383078347374592 |
---|---|
author | Zidar, Nina Odar, Katarina Glavač, Damjan Jerše, Maja Zupanc, Tomaž Štajer, Dušan |
author_facet | Zidar, Nina Odar, Katarina Glavač, Damjan Jerše, Maja Zupanc, Tomaž Štajer, Dušan |
author_sort | Zidar, Nina |
collection | PubMed |
description | Cyclooxygenase (COX) is a key enzyme in prostanoid synthesis. It exists in two isoforms, COX-1 and COX-2. COX-1 is referred to as a ‘constitutive isoform’, and is considered to be expressed in most tissues under basal conditions. In contrast, COX-2 is referred to as an ‘inducible isoform’, which is believed to be undetectable in most normal tissues, but can be up-regulated during various conditions, many of them pathological. Even though the role of COX in homeostasis and disease in now well appreciated, controversial information is available concerning the distribution of COX isoforms in normal human tissues. There is mounting evidence that it is much more complex than generally believed. Our aim was therefore to analyse the expression and distribution of COX isoforms in normal human tissues, using immunohistochemistry, Western blotting and real-time RT-PCR. Autopsy samples from 20 healthy trauma victims and samples from 48 biopsy surgical specimens were included. COX-1 was found in blood vessels, interstitial cells, smooth muscle cells, platelets and mesothelial cells. In contrast, COX-2 was found predominantly in the parenchymal cells of many tissues, with few exceptions, for example the heart. Our results confirm the hypothesis that the distribution of COX isoforms in healthy tissues is much more complex than generally believed. This and previous studies indicate that both isoforms, not only COX-1, are present in many normal human tissues, and that both isoforms, not only COX-2, are up-regulated in various pathological conditions. We may have to revise the concept of ‘constitutive’ and ‘inducible’ COX isoforms. |
format | Online Article Text |
id | pubmed-4516524 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | John Wiley & Sons, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-45165242015-08-03 Cyclooxygenase in normal human tissues – is COX-1 really a constitutive isoform, and COX-2 an inducible isoform? Zidar, Nina Odar, Katarina Glavač, Damjan Jerše, Maja Zupanc, Tomaž Štajer, Dušan J Cell Mol Med Molecular Medicine Cyclooxygenase (COX) is a key enzyme in prostanoid synthesis. It exists in two isoforms, COX-1 and COX-2. COX-1 is referred to as a ‘constitutive isoform’, and is considered to be expressed in most tissues under basal conditions. In contrast, COX-2 is referred to as an ‘inducible isoform’, which is believed to be undetectable in most normal tissues, but can be up-regulated during various conditions, many of them pathological. Even though the role of COX in homeostasis and disease in now well appreciated, controversial information is available concerning the distribution of COX isoforms in normal human tissues. There is mounting evidence that it is much more complex than generally believed. Our aim was therefore to analyse the expression and distribution of COX isoforms in normal human tissues, using immunohistochemistry, Western blotting and real-time RT-PCR. Autopsy samples from 20 healthy trauma victims and samples from 48 biopsy surgical specimens were included. COX-1 was found in blood vessels, interstitial cells, smooth muscle cells, platelets and mesothelial cells. In contrast, COX-2 was found predominantly in the parenchymal cells of many tissues, with few exceptions, for example the heart. Our results confirm the hypothesis that the distribution of COX isoforms in healthy tissues is much more complex than generally believed. This and previous studies indicate that both isoforms, not only COX-1, are present in many normal human tissues, and that both isoforms, not only COX-2, are up-regulated in various pathological conditions. We may have to revise the concept of ‘constitutive’ and ‘inducible’ COX isoforms. John Wiley & Sons, Ltd 2009-09 2008-07-24 /pmc/articles/PMC4516524/ /pubmed/18657230 http://dx.doi.org/10.1111/j.1582-4934.2008.00430.x Text en © 2008 The Authors Journal compilation © 2009 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd |
spellingShingle | Molecular Medicine Zidar, Nina Odar, Katarina Glavač, Damjan Jerše, Maja Zupanc, Tomaž Štajer, Dušan Cyclooxygenase in normal human tissues – is COX-1 really a constitutive isoform, and COX-2 an inducible isoform? |
title | Cyclooxygenase in normal human tissues – is COX-1 really a constitutive isoform, and COX-2 an inducible isoform? |
title_full | Cyclooxygenase in normal human tissues – is COX-1 really a constitutive isoform, and COX-2 an inducible isoform? |
title_fullStr | Cyclooxygenase in normal human tissues – is COX-1 really a constitutive isoform, and COX-2 an inducible isoform? |
title_full_unstemmed | Cyclooxygenase in normal human tissues – is COX-1 really a constitutive isoform, and COX-2 an inducible isoform? |
title_short | Cyclooxygenase in normal human tissues – is COX-1 really a constitutive isoform, and COX-2 an inducible isoform? |
title_sort | cyclooxygenase in normal human tissues – is cox-1 really a constitutive isoform, and cox-2 an inducible isoform? |
topic | Molecular Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4516524/ https://www.ncbi.nlm.nih.gov/pubmed/18657230 http://dx.doi.org/10.1111/j.1582-4934.2008.00430.x |
work_keys_str_mv | AT zidarnina cyclooxygenaseinnormalhumantissuesiscox1reallyaconstitutiveisoformandcox2aninducibleisoform AT odarkatarina cyclooxygenaseinnormalhumantissuesiscox1reallyaconstitutiveisoformandcox2aninducibleisoform AT glavacdamjan cyclooxygenaseinnormalhumantissuesiscox1reallyaconstitutiveisoformandcox2aninducibleisoform AT jersemaja cyclooxygenaseinnormalhumantissuesiscox1reallyaconstitutiveisoformandcox2aninducibleisoform AT zupanctomaz cyclooxygenaseinnormalhumantissuesiscox1reallyaconstitutiveisoformandcox2aninducibleisoform AT stajerdusan cyclooxygenaseinnormalhumantissuesiscox1reallyaconstitutiveisoformandcox2aninducibleisoform |