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Citrulline Supplementation Improves Organ Perfusion and Arginine Availability under Conditions with Enhanced Arginase Activity

Enhanced arginase-induced arginine consumption is believed to play a key role in the pathogenesis of sickle cell disease-induced end organ failure. Enhancement of arginine availability with l-arginine supplementation exhibited less consistent results; however, l-citrulline, the precursor of l-argini...

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Autores principales: Wijnands, Karolina A.P., Meesters, Dennis M., van Barneveld, Kevin W.Y., Visschers, Ruben G.J., Briedé, Jacob J., Vandendriessche, Benjamin, van Eijk, Hans M.H., Bessems, Babs A.F.M., van den Hoven, Nadine, von Wintersdorff, Christian J.H., Brouckaert, Peter, Bouvy, Nicole D., Lamers, Wouter H., Cauwels, Anje, Poeze, Martijn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4516994/
https://www.ncbi.nlm.nih.gov/pubmed/26132994
http://dx.doi.org/10.3390/nu7075217
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author Wijnands, Karolina A.P.
Meesters, Dennis M.
van Barneveld, Kevin W.Y.
Visschers, Ruben G.J.
Briedé, Jacob J.
Vandendriessche, Benjamin
van Eijk, Hans M.H.
Bessems, Babs A.F.M.
van den Hoven, Nadine
von Wintersdorff, Christian J.H.
Brouckaert, Peter
Bouvy, Nicole D.
Lamers, Wouter H.
Cauwels, Anje
Poeze, Martijn
author_facet Wijnands, Karolina A.P.
Meesters, Dennis M.
van Barneveld, Kevin W.Y.
Visschers, Ruben G.J.
Briedé, Jacob J.
Vandendriessche, Benjamin
van Eijk, Hans M.H.
Bessems, Babs A.F.M.
van den Hoven, Nadine
von Wintersdorff, Christian J.H.
Brouckaert, Peter
Bouvy, Nicole D.
Lamers, Wouter H.
Cauwels, Anje
Poeze, Martijn
author_sort Wijnands, Karolina A.P.
collection PubMed
description Enhanced arginase-induced arginine consumption is believed to play a key role in the pathogenesis of sickle cell disease-induced end organ failure. Enhancement of arginine availability with l-arginine supplementation exhibited less consistent results; however, l-citrulline, the precursor of l-arginine, may be a promising alternative. In this study, we determined the effects of l-citrulline compared to l-arginine supplementation on arginine-nitric oxide (NO) metabolism, arginine availability and microcirculation in a murine model with acutely-enhanced arginase activity. The effects were measured in six groups of mice (n = 8 each) injected intraperitoneally with sterile saline or arginase (1000 IE/mouse) with or without being separately injected with l-citrulline or l-arginine 1 h prior to assessment of the microcirculation with side stream dark-field (SDF)-imaging or in vivo NO-production with electron spin resonance (ESR) spectroscopy. Arginase injection caused a decrease in plasma and tissue arginine concentrations. l-arginine and l-citrulline supplementation both enhanced plasma and tissue arginine concentrations in arginase-injected mice. However, only the citrulline supplementation increased NO production and improved microcirculatory flow in arginase-injected mice. In conclusion, the present study provides for the first time in vivo experimental evidence that l-citrulline, and not l-arginine supplementation, improves the end organ microcirculation during conditions with acute arginase-induced arginine deficiency by increasing the NO concentration in tissues.
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spelling pubmed-45169942015-07-30 Citrulline Supplementation Improves Organ Perfusion and Arginine Availability under Conditions with Enhanced Arginase Activity Wijnands, Karolina A.P. Meesters, Dennis M. van Barneveld, Kevin W.Y. Visschers, Ruben G.J. Briedé, Jacob J. Vandendriessche, Benjamin van Eijk, Hans M.H. Bessems, Babs A.F.M. van den Hoven, Nadine von Wintersdorff, Christian J.H. Brouckaert, Peter Bouvy, Nicole D. Lamers, Wouter H. Cauwels, Anje Poeze, Martijn Nutrients Article Enhanced arginase-induced arginine consumption is believed to play a key role in the pathogenesis of sickle cell disease-induced end organ failure. Enhancement of arginine availability with l-arginine supplementation exhibited less consistent results; however, l-citrulline, the precursor of l-arginine, may be a promising alternative. In this study, we determined the effects of l-citrulline compared to l-arginine supplementation on arginine-nitric oxide (NO) metabolism, arginine availability and microcirculation in a murine model with acutely-enhanced arginase activity. The effects were measured in six groups of mice (n = 8 each) injected intraperitoneally with sterile saline or arginase (1000 IE/mouse) with or without being separately injected with l-citrulline or l-arginine 1 h prior to assessment of the microcirculation with side stream dark-field (SDF)-imaging or in vivo NO-production with electron spin resonance (ESR) spectroscopy. Arginase injection caused a decrease in plasma and tissue arginine concentrations. l-arginine and l-citrulline supplementation both enhanced plasma and tissue arginine concentrations in arginase-injected mice. However, only the citrulline supplementation increased NO production and improved microcirculatory flow in arginase-injected mice. In conclusion, the present study provides for the first time in vivo experimental evidence that l-citrulline, and not l-arginine supplementation, improves the end organ microcirculation during conditions with acute arginase-induced arginine deficiency by increasing the NO concentration in tissues. MDPI 2015-06-29 /pmc/articles/PMC4516994/ /pubmed/26132994 http://dx.doi.org/10.3390/nu7075217 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wijnands, Karolina A.P.
Meesters, Dennis M.
van Barneveld, Kevin W.Y.
Visschers, Ruben G.J.
Briedé, Jacob J.
Vandendriessche, Benjamin
van Eijk, Hans M.H.
Bessems, Babs A.F.M.
van den Hoven, Nadine
von Wintersdorff, Christian J.H.
Brouckaert, Peter
Bouvy, Nicole D.
Lamers, Wouter H.
Cauwels, Anje
Poeze, Martijn
Citrulline Supplementation Improves Organ Perfusion and Arginine Availability under Conditions with Enhanced Arginase Activity
title Citrulline Supplementation Improves Organ Perfusion and Arginine Availability under Conditions with Enhanced Arginase Activity
title_full Citrulline Supplementation Improves Organ Perfusion and Arginine Availability under Conditions with Enhanced Arginase Activity
title_fullStr Citrulline Supplementation Improves Organ Perfusion and Arginine Availability under Conditions with Enhanced Arginase Activity
title_full_unstemmed Citrulline Supplementation Improves Organ Perfusion and Arginine Availability under Conditions with Enhanced Arginase Activity
title_short Citrulline Supplementation Improves Organ Perfusion and Arginine Availability under Conditions with Enhanced Arginase Activity
title_sort citrulline supplementation improves organ perfusion and arginine availability under conditions with enhanced arginase activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4516994/
https://www.ncbi.nlm.nih.gov/pubmed/26132994
http://dx.doi.org/10.3390/nu7075217
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