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Ellagic Acid Prevents L-NAME-Induced Hypertension via Restoration of eNOS and p47(phox) Expression in Rats

The effect of ellagic acid on oxidative stress and hypertension induced by N(ω)-Nitro-l-arginine methyl ester hydrochloride (L-NAME) was investigated. Male Sprague-Dawley rats were administrated with L-NAME (40 mg/kg/day) for five weeks. L-NAME induced high systolic blood pressure (SBP) and increase...

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Autores principales: Berkban, Thewarid, Boonprom, Pattanapong, Bunbupha, Sarawoot, Umka Welbat, Jariya, Kukongviriyapan, Upa, Kukongviriyapan, Veerapol, Pakdeechote, Poungrat, Prachaney, Parichat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4516999/
https://www.ncbi.nlm.nih.gov/pubmed/26133972
http://dx.doi.org/10.3390/nu7075222
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author Berkban, Thewarid
Boonprom, Pattanapong
Bunbupha, Sarawoot
Umka Welbat, Jariya
Kukongviriyapan, Upa
Kukongviriyapan, Veerapol
Pakdeechote, Poungrat
Prachaney, Parichat
author_facet Berkban, Thewarid
Boonprom, Pattanapong
Bunbupha, Sarawoot
Umka Welbat, Jariya
Kukongviriyapan, Upa
Kukongviriyapan, Veerapol
Pakdeechote, Poungrat
Prachaney, Parichat
author_sort Berkban, Thewarid
collection PubMed
description The effect of ellagic acid on oxidative stress and hypertension induced by N(ω)-Nitro-l-arginine methyl ester hydrochloride (L-NAME) was investigated. Male Sprague-Dawley rats were administrated with L-NAME (40 mg/kg/day) for five weeks. L-NAME induced high systolic blood pressure (SBP) and increased heart rate (HR), hindlimb vascular resistance (HVR) and oxidative stress. Concurrent treatment with ellagic acid (7.5 or 15 mg/kg) prevented these alterations. Co-treatment with ellagic acid was associated with up-regulation of endothelial nitric oxide synthase (eNOS) protein production and alleviation of oxidative stress as indicated by decreased superoxide production in the vascular tissue, reduced plasma malondialdehyde levels, reduced NADPH oxidase subunit p47(phox) expression and increased plasma nitrate/nitrite levels. Our results indicate that ellagic acid attenuates hypertension by reducing NADPH oxidase subunit p47(phox) expression, which prevents oxidative stress and restores NO bioavailability.
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spelling pubmed-45169992015-07-30 Ellagic Acid Prevents L-NAME-Induced Hypertension via Restoration of eNOS and p47(phox) Expression in Rats Berkban, Thewarid Boonprom, Pattanapong Bunbupha, Sarawoot Umka Welbat, Jariya Kukongviriyapan, Upa Kukongviriyapan, Veerapol Pakdeechote, Poungrat Prachaney, Parichat Nutrients Article The effect of ellagic acid on oxidative stress and hypertension induced by N(ω)-Nitro-l-arginine methyl ester hydrochloride (L-NAME) was investigated. Male Sprague-Dawley rats were administrated with L-NAME (40 mg/kg/day) for five weeks. L-NAME induced high systolic blood pressure (SBP) and increased heart rate (HR), hindlimb vascular resistance (HVR) and oxidative stress. Concurrent treatment with ellagic acid (7.5 or 15 mg/kg) prevented these alterations. Co-treatment with ellagic acid was associated with up-regulation of endothelial nitric oxide synthase (eNOS) protein production and alleviation of oxidative stress as indicated by decreased superoxide production in the vascular tissue, reduced plasma malondialdehyde levels, reduced NADPH oxidase subunit p47(phox) expression and increased plasma nitrate/nitrite levels. Our results indicate that ellagic acid attenuates hypertension by reducing NADPH oxidase subunit p47(phox) expression, which prevents oxidative stress and restores NO bioavailability. MDPI 2015-06-30 /pmc/articles/PMC4516999/ /pubmed/26133972 http://dx.doi.org/10.3390/nu7075222 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Berkban, Thewarid
Boonprom, Pattanapong
Bunbupha, Sarawoot
Umka Welbat, Jariya
Kukongviriyapan, Upa
Kukongviriyapan, Veerapol
Pakdeechote, Poungrat
Prachaney, Parichat
Ellagic Acid Prevents L-NAME-Induced Hypertension via Restoration of eNOS and p47(phox) Expression in Rats
title Ellagic Acid Prevents L-NAME-Induced Hypertension via Restoration of eNOS and p47(phox) Expression in Rats
title_full Ellagic Acid Prevents L-NAME-Induced Hypertension via Restoration of eNOS and p47(phox) Expression in Rats
title_fullStr Ellagic Acid Prevents L-NAME-Induced Hypertension via Restoration of eNOS and p47(phox) Expression in Rats
title_full_unstemmed Ellagic Acid Prevents L-NAME-Induced Hypertension via Restoration of eNOS and p47(phox) Expression in Rats
title_short Ellagic Acid Prevents L-NAME-Induced Hypertension via Restoration of eNOS and p47(phox) Expression in Rats
title_sort ellagic acid prevents l-name-induced hypertension via restoration of enos and p47(phox) expression in rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4516999/
https://www.ncbi.nlm.nih.gov/pubmed/26133972
http://dx.doi.org/10.3390/nu7075222
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