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Genome sequence of Clostridium sporogenes DSM 795(T), an amino acid-degrading, nontoxic surrogate of neurotoxin-producing Clostridium botulinum

Clostridium sporogenes DSM 795 is the type strain of the species Clostridium sporogenes, first described by Metchnikoff in 1908. It is a Gram-positive, rod-shaped, anaerobic bacterium isolated from human faeces and belongs to the proteolytic branch of clostridia. C. sporogenes attracts special inter...

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Autores principales: Poehlein, Anja, Riegel, Karin, König, Sandra M, Leimbach, Andreas, Daniel, Rolf, Dürre, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4517662/
https://www.ncbi.nlm.nih.gov/pubmed/26221421
http://dx.doi.org/10.1186/s40793-015-0016-y
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author Poehlein, Anja
Riegel, Karin
König, Sandra M
Leimbach, Andreas
Daniel, Rolf
Dürre, Peter
author_facet Poehlein, Anja
Riegel, Karin
König, Sandra M
Leimbach, Andreas
Daniel, Rolf
Dürre, Peter
author_sort Poehlein, Anja
collection PubMed
description Clostridium sporogenes DSM 795 is the type strain of the species Clostridium sporogenes, first described by Metchnikoff in 1908. It is a Gram-positive, rod-shaped, anaerobic bacterium isolated from human faeces and belongs to the proteolytic branch of clostridia. C. sporogenes attracts special interest because of its potential use in a bacterial therapy for certain cancer types. Genome sequencing and annotation revealed several gene clusters coding for proteins involved in anaerobic degradation of amino acids, such as glycine and betaine via Stickland reaction. Genome comparison showed that C. sporogenes is closely related to C. botulinum. The genome of C. sporogenes DSM 795 consists of a circular chromosome of 4.1 Mb with an overall GC content of 27.81 mol% harboring 3,744 protein-coding genes, and 80 RNAs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40793-015-0016-y) contains supplementary material, which is available to authorized users.
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spelling pubmed-45176622015-07-29 Genome sequence of Clostridium sporogenes DSM 795(T), an amino acid-degrading, nontoxic surrogate of neurotoxin-producing Clostridium botulinum Poehlein, Anja Riegel, Karin König, Sandra M Leimbach, Andreas Daniel, Rolf Dürre, Peter Stand Genomic Sci Extended Genome Report Clostridium sporogenes DSM 795 is the type strain of the species Clostridium sporogenes, first described by Metchnikoff in 1908. It is a Gram-positive, rod-shaped, anaerobic bacterium isolated from human faeces and belongs to the proteolytic branch of clostridia. C. sporogenes attracts special interest because of its potential use in a bacterial therapy for certain cancer types. Genome sequencing and annotation revealed several gene clusters coding for proteins involved in anaerobic degradation of amino acids, such as glycine and betaine via Stickland reaction. Genome comparison showed that C. sporogenes is closely related to C. botulinum. The genome of C. sporogenes DSM 795 consists of a circular chromosome of 4.1 Mb with an overall GC content of 27.81 mol% harboring 3,744 protein-coding genes, and 80 RNAs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s40793-015-0016-y) contains supplementary material, which is available to authorized users. BioMed Central 2015-07-21 /pmc/articles/PMC4517662/ /pubmed/26221421 http://dx.doi.org/10.1186/s40793-015-0016-y Text en © Poehlein et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Extended Genome Report
Poehlein, Anja
Riegel, Karin
König, Sandra M
Leimbach, Andreas
Daniel, Rolf
Dürre, Peter
Genome sequence of Clostridium sporogenes DSM 795(T), an amino acid-degrading, nontoxic surrogate of neurotoxin-producing Clostridium botulinum
title Genome sequence of Clostridium sporogenes DSM 795(T), an amino acid-degrading, nontoxic surrogate of neurotoxin-producing Clostridium botulinum
title_full Genome sequence of Clostridium sporogenes DSM 795(T), an amino acid-degrading, nontoxic surrogate of neurotoxin-producing Clostridium botulinum
title_fullStr Genome sequence of Clostridium sporogenes DSM 795(T), an amino acid-degrading, nontoxic surrogate of neurotoxin-producing Clostridium botulinum
title_full_unstemmed Genome sequence of Clostridium sporogenes DSM 795(T), an amino acid-degrading, nontoxic surrogate of neurotoxin-producing Clostridium botulinum
title_short Genome sequence of Clostridium sporogenes DSM 795(T), an amino acid-degrading, nontoxic surrogate of neurotoxin-producing Clostridium botulinum
title_sort genome sequence of clostridium sporogenes dsm 795(t), an amino acid-degrading, nontoxic surrogate of neurotoxin-producing clostridium botulinum
topic Extended Genome Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4517662/
https://www.ncbi.nlm.nih.gov/pubmed/26221421
http://dx.doi.org/10.1186/s40793-015-0016-y
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