Genomic Assays for Identification of Chikungunya Virus in Blood Donors, Puerto Rico, 2014

A newly developed transcription-mediated amplification assay was used to detect chikungunya virus infection in 3 of 557 asymptomatic donors (0.54%) from Puerto Rico during the 2014–2015 Caribbean epidemic. Viral detection was confirmed by using PCR, microarray, and next-generation sequencing. Molecu...

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Detalles Bibliográficos
Autores principales: Chiu, Charles Y., Bres, Vanessa, Yu, Guixia, Krysztof, David, Naccache, Samia N., Lee, Deanna, Pfeil, Jacob, Linnen, Jeffrey M., Stramer, Susan L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2015
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4517739/
https://www.ncbi.nlm.nih.gov/pubmed/26196378
http://dx.doi.org/10.3201/eid2108.150458
Descripción
Sumario:A newly developed transcription-mediated amplification assay was used to detect chikungunya virus infection in 3 of 557 asymptomatic donors (0.54%) from Puerto Rico during the 2014–2015 Caribbean epidemic. Viral detection was confirmed by using PCR, microarray, and next-generation sequencing. Molecular clock analysis dated the emergence of the Puerto Rico strains to early 2013.

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