Genomic Assays for Identification of Chikungunya Virus in Blood Donors, Puerto Rico, 2014

A newly developed transcription-mediated amplification assay was used to detect chikungunya virus infection in 3 of 557 asymptomatic donors (0.54%) from Puerto Rico during the 2014–2015 Caribbean epidemic. Viral detection was confirmed by using PCR, microarray, and next-generation sequencing. Molecu...

Descripción completa

Detalles Bibliográficos
Autores principales: Chiu, Charles Y., Bres, Vanessa, Yu, Guixia, Krysztof, David, Naccache, Samia N., Lee, Deanna, Pfeil, Jacob, Linnen, Jeffrey M., Stramer, Susan L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2015
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4517739/
https://www.ncbi.nlm.nih.gov/pubmed/26196378
http://dx.doi.org/10.3201/eid2108.150458
_version_ 1782383229726097408
author Chiu, Charles Y.
Bres, Vanessa
Yu, Guixia
Krysztof, David
Naccache, Samia N.
Lee, Deanna
Pfeil, Jacob
Linnen, Jeffrey M.
Stramer, Susan L.
author_facet Chiu, Charles Y.
Bres, Vanessa
Yu, Guixia
Krysztof, David
Naccache, Samia N.
Lee, Deanna
Pfeil, Jacob
Linnen, Jeffrey M.
Stramer, Susan L.
author_sort Chiu, Charles Y.
collection PubMed
description A newly developed transcription-mediated amplification assay was used to detect chikungunya virus infection in 3 of 557 asymptomatic donors (0.54%) from Puerto Rico during the 2014–2015 Caribbean epidemic. Viral detection was confirmed by using PCR, microarray, and next-generation sequencing. Molecular clock analysis dated the emergence of the Puerto Rico strains to early 2013.
format Online
Article
Text
id pubmed-4517739
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Centers for Disease Control and Prevention
record_format MEDLINE/PubMed
spelling pubmed-45177392015-08-01 Genomic Assays for Identification of Chikungunya Virus in Blood Donors, Puerto Rico, 2014 Chiu, Charles Y. Bres, Vanessa Yu, Guixia Krysztof, David Naccache, Samia N. Lee, Deanna Pfeil, Jacob Linnen, Jeffrey M. Stramer, Susan L. Emerg Infect Dis Dispatch A newly developed transcription-mediated amplification assay was used to detect chikungunya virus infection in 3 of 557 asymptomatic donors (0.54%) from Puerto Rico during the 2014–2015 Caribbean epidemic. Viral detection was confirmed by using PCR, microarray, and next-generation sequencing. Molecular clock analysis dated the emergence of the Puerto Rico strains to early 2013. Centers for Disease Control and Prevention 2015-08 /pmc/articles/PMC4517739/ /pubmed/26196378 http://dx.doi.org/10.3201/eid2108.150458 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Chiu, Charles Y.
Bres, Vanessa
Yu, Guixia
Krysztof, David
Naccache, Samia N.
Lee, Deanna
Pfeil, Jacob
Linnen, Jeffrey M.
Stramer, Susan L.
Genomic Assays for Identification of Chikungunya Virus in Blood Donors, Puerto Rico, 2014
title Genomic Assays for Identification of Chikungunya Virus in Blood Donors, Puerto Rico, 2014
title_full Genomic Assays for Identification of Chikungunya Virus in Blood Donors, Puerto Rico, 2014
title_fullStr Genomic Assays for Identification of Chikungunya Virus in Blood Donors, Puerto Rico, 2014
title_full_unstemmed Genomic Assays for Identification of Chikungunya Virus in Blood Donors, Puerto Rico, 2014
title_short Genomic Assays for Identification of Chikungunya Virus in Blood Donors, Puerto Rico, 2014
title_sort genomic assays for identification of chikungunya virus in blood donors, puerto rico, 2014
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4517739/
https://www.ncbi.nlm.nih.gov/pubmed/26196378
http://dx.doi.org/10.3201/eid2108.150458
work_keys_str_mv AT chiucharlesy genomicassaysforidentificationofchikungunyavirusinblooddonorspuertorico2014
AT bresvanessa genomicassaysforidentificationofchikungunyavirusinblooddonorspuertorico2014
AT yuguixia genomicassaysforidentificationofchikungunyavirusinblooddonorspuertorico2014
AT krysztofdavid genomicassaysforidentificationofchikungunyavirusinblooddonorspuertorico2014
AT naccachesamian genomicassaysforidentificationofchikungunyavirusinblooddonorspuertorico2014
AT leedeanna genomicassaysforidentificationofchikungunyavirusinblooddonorspuertorico2014
AT pfeiljacob genomicassaysforidentificationofchikungunyavirusinblooddonorspuertorico2014
AT linnenjeffreym genomicassaysforidentificationofchikungunyavirusinblooddonorspuertorico2014
AT stramersusanl genomicassaysforidentificationofchikungunyavirusinblooddonorspuertorico2014

Ejemplares similares