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Characterization of Antibiotic Resistance Gene Abundance and Microbiota Composition in Feces of Organic and Conventional Pigs from Four EU Countries

One of the recent trends in animal production is the revival of interest in organic farming. The increased consumer interest in organic animal farming is mainly due to concerns about animal welfare and the use of antibiotics in conventional farming. On the other hand, providing animals with a more n...

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Autores principales: Gerzova, Lenka, Babak, Vladimir, Sedlar, Karel, Faldynova, Marcela, Videnska, Petra, Cejkova, Darina, Jensen, Annette Nygaard, Denis, Martine, Kerouanton, Annaelle, Ricci, Antonia, Cibin, Veronica, Österberg, Julia, Rychlik, Ivan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4517930/
https://www.ncbi.nlm.nih.gov/pubmed/26218075
http://dx.doi.org/10.1371/journal.pone.0132892
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author Gerzova, Lenka
Babak, Vladimir
Sedlar, Karel
Faldynova, Marcela
Videnska, Petra
Cejkova, Darina
Jensen, Annette Nygaard
Denis, Martine
Kerouanton, Annaelle
Ricci, Antonia
Cibin, Veronica
Österberg, Julia
Rychlik, Ivan
author_facet Gerzova, Lenka
Babak, Vladimir
Sedlar, Karel
Faldynova, Marcela
Videnska, Petra
Cejkova, Darina
Jensen, Annette Nygaard
Denis, Martine
Kerouanton, Annaelle
Ricci, Antonia
Cibin, Veronica
Österberg, Julia
Rychlik, Ivan
author_sort Gerzova, Lenka
collection PubMed
description One of the recent trends in animal production is the revival of interest in organic farming. The increased consumer interest in organic animal farming is mainly due to concerns about animal welfare and the use of antibiotics in conventional farming. On the other hand, providing animals with a more natural lifestyle implies their increased exposure to environmental sources of different microorganisms including pathogens. To address these concerns, we determined the abundance of antibiotic resistance and diversity within fecal microbiota in pigs kept under conventional and organic farming systems in Sweden, Denmark, France and Italy. The abundance of sul1, sul2, strA, tet(A), tet(B) and cat antibiotic resistance genes was determined in 468 samples by real-time PCR and the fecal microbiota diversity was characterized in 48 selected samples by pyrosequencing of V3/V4 regions of 16S rRNA. Contrary to our expectations, there were no extensive differences between the abundance of tested antibiotic resistance genes in microbiota originating from organic or conventionally housed pigs within individual countries. There were also no differences in the microbiota composition of organic and conventional pigs. The only significant difference was the difference in the abundance of antibiotic resistance genes in the samples from different countries. Fecal microbiota in the samples originating from southern European countries (Italy, France) exhibited significantly higher antibiotic resistance gene abundance than those from northern parts of Europe (Denmark, Sweden). Therefore, the geographical location of the herd influenced the antibiotic resistance in the fecal microbiota more than farm’s status as organic or conventional.
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spelling pubmed-45179302015-07-31 Characterization of Antibiotic Resistance Gene Abundance and Microbiota Composition in Feces of Organic and Conventional Pigs from Four EU Countries Gerzova, Lenka Babak, Vladimir Sedlar, Karel Faldynova, Marcela Videnska, Petra Cejkova, Darina Jensen, Annette Nygaard Denis, Martine Kerouanton, Annaelle Ricci, Antonia Cibin, Veronica Österberg, Julia Rychlik, Ivan PLoS One Research Article One of the recent trends in animal production is the revival of interest in organic farming. The increased consumer interest in organic animal farming is mainly due to concerns about animal welfare and the use of antibiotics in conventional farming. On the other hand, providing animals with a more natural lifestyle implies their increased exposure to environmental sources of different microorganisms including pathogens. To address these concerns, we determined the abundance of antibiotic resistance and diversity within fecal microbiota in pigs kept under conventional and organic farming systems in Sweden, Denmark, France and Italy. The abundance of sul1, sul2, strA, tet(A), tet(B) and cat antibiotic resistance genes was determined in 468 samples by real-time PCR and the fecal microbiota diversity was characterized in 48 selected samples by pyrosequencing of V3/V4 regions of 16S rRNA. Contrary to our expectations, there were no extensive differences between the abundance of tested antibiotic resistance genes in microbiota originating from organic or conventionally housed pigs within individual countries. There were also no differences in the microbiota composition of organic and conventional pigs. The only significant difference was the difference in the abundance of antibiotic resistance genes in the samples from different countries. Fecal microbiota in the samples originating from southern European countries (Italy, France) exhibited significantly higher antibiotic resistance gene abundance than those from northern parts of Europe (Denmark, Sweden). Therefore, the geographical location of the herd influenced the antibiotic resistance in the fecal microbiota more than farm’s status as organic or conventional. Public Library of Science 2015-07-28 /pmc/articles/PMC4517930/ /pubmed/26218075 http://dx.doi.org/10.1371/journal.pone.0132892 Text en © 2015 Gerzova et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Gerzova, Lenka
Babak, Vladimir
Sedlar, Karel
Faldynova, Marcela
Videnska, Petra
Cejkova, Darina
Jensen, Annette Nygaard
Denis, Martine
Kerouanton, Annaelle
Ricci, Antonia
Cibin, Veronica
Österberg, Julia
Rychlik, Ivan
Characterization of Antibiotic Resistance Gene Abundance and Microbiota Composition in Feces of Organic and Conventional Pigs from Four EU Countries
title Characterization of Antibiotic Resistance Gene Abundance and Microbiota Composition in Feces of Organic and Conventional Pigs from Four EU Countries
title_full Characterization of Antibiotic Resistance Gene Abundance and Microbiota Composition in Feces of Organic and Conventional Pigs from Four EU Countries
title_fullStr Characterization of Antibiotic Resistance Gene Abundance and Microbiota Composition in Feces of Organic and Conventional Pigs from Four EU Countries
title_full_unstemmed Characterization of Antibiotic Resistance Gene Abundance and Microbiota Composition in Feces of Organic and Conventional Pigs from Four EU Countries
title_short Characterization of Antibiotic Resistance Gene Abundance and Microbiota Composition in Feces of Organic and Conventional Pigs from Four EU Countries
title_sort characterization of antibiotic resistance gene abundance and microbiota composition in feces of organic and conventional pigs from four eu countries
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4517930/
https://www.ncbi.nlm.nih.gov/pubmed/26218075
http://dx.doi.org/10.1371/journal.pone.0132892
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