All that is gold does not glitter? Age, taxonomy, and ancient plant DNA quality

More than 600 herbarium samples from four distantly related groups of flowering plants were used for DNA extraction and subsequent measurements of DNA purity and concentration. We did not find any significant relation between DNA purity and the age of the sample. However, DNA yields were different b...

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Detalles Bibliográficos
Autores principales: Choi, JinHee, Lee, HyeJi, Shipunov, Alexey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2015
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4517958/
https://www.ncbi.nlm.nih.gov/pubmed/26244108
http://dx.doi.org/10.7717/peerj.1087
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author Choi, JinHee
Lee, HyeJi
Shipunov, Alexey
author_facet Choi, JinHee
Lee, HyeJi
Shipunov, Alexey
author_sort Choi, JinHee
collection PubMed
description More than 600 herbarium samples from four distantly related groups of flowering plants were used for DNA extraction and subsequent measurements of DNA purity and concentration. We did not find any significant relation between DNA purity and the age of the sample. However, DNA yields were different between plant groups studied. We believe that there there should be no reservations about “old” samples if the goal is to extract more DNA of better purity. We argue that the older herbarium samples are the mine for the future DNA studies, and have the value not less than the “fresh” specimens.
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spelling pubmed-45179582015-08-04 All that is gold does not glitter? Age, taxonomy, and ancient plant DNA quality Choi, JinHee Lee, HyeJi Shipunov, Alexey PeerJ Plant Science More than 600 herbarium samples from four distantly related groups of flowering plants were used for DNA extraction and subsequent measurements of DNA purity and concentration. We did not find any significant relation between DNA purity and the age of the sample. However, DNA yields were different between plant groups studied. We believe that there there should be no reservations about “old” samples if the goal is to extract more DNA of better purity. We argue that the older herbarium samples are the mine for the future DNA studies, and have the value not less than the “fresh” specimens. PeerJ Inc. 2015-07-23 /pmc/articles/PMC4517958/ /pubmed/26244108 http://dx.doi.org/10.7717/peerj.1087 Text en © 2015 Choi et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Plant Science
Choi, JinHee
Lee, HyeJi
Shipunov, Alexey
All that is gold does not glitter? Age, taxonomy, and ancient plant DNA quality
title All that is gold does not glitter? Age, taxonomy, and ancient plant DNA quality
title_full All that is gold does not glitter? Age, taxonomy, and ancient plant DNA quality
title_fullStr All that is gold does not glitter? Age, taxonomy, and ancient plant DNA quality
title_full_unstemmed All that is gold does not glitter? Age, taxonomy, and ancient plant DNA quality
title_short All that is gold does not glitter? Age, taxonomy, and ancient plant DNA quality
title_sort all that is gold does not glitter? age, taxonomy, and ancient plant dna quality
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4517958/
https://www.ncbi.nlm.nih.gov/pubmed/26244108
http://dx.doi.org/10.7717/peerj.1087
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