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Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry
In previous studies, mebudipine, a dihydropyridine calcium channel blocker, showed a considerable potential to be used in cardiovascular diseases. The aim of the current study was to develop a valid method using reversed-phase high performance liquid chromatography coupled with electrospray ionizati...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Shaheed Beheshti University of Medical Sciences
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4518102/ https://www.ncbi.nlm.nih.gov/pubmed/26330862 |
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author | Asgari, Arezoo Kobarfard, Farzad Keyhanfar, Fariborz Mohebbi, Shohreh Noubarani, Maryam |
author_facet | Asgari, Arezoo Kobarfard, Farzad Keyhanfar, Fariborz Mohebbi, Shohreh Noubarani, Maryam |
author_sort | Asgari, Arezoo |
collection | PubMed |
description | In previous studies, mebudipine, a dihydropyridine calcium channel blocker, showed a considerable potential to be used in cardiovascular diseases. The aim of the current study was to develop a valid method using reversed-phase high performance liquid chromatography coupled with electrospray ionization mass spectrometry to assay mebudipine in the human plasma. Separation was achieved on a Zorbax Eclipse(®) C18 analytical column using a mobile phase consisted of methanol/water (90:10, v/v). The flow rate was 0.6 mL/min and carbamazepine was used as an internal standard (IS). This method involved the use of [M +Na](+) ions of mebudipine and IS at m/z 411 and 259, respectively with the selected ion monitoring (SIM) mode. There were no interfering peaks from endogenous components in blank plasma chromatograms. Standard curves were linear (r(2)>0.99) between 5 to 100 ng/mL. The mean extraction efficiency was about 84% and the limit of quantification for mebudipine was 5 ng/mL in plasma. The coefficient of variation and error at all of the intra-day and inter-day assessments were less than 11%. The results indicated that this method is a fast, accurate, sensitive, selective and reliable method for the determination of mebudipine in the human plasma. The assay method has been successfully used to estimate plasma concentration of mebudipine after the oral administration of 2.5 mg tablet in healthy adults. |
format | Online Article Text |
id | pubmed-4518102 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Shaheed Beheshti University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-45181022015-09-01 Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry Asgari, Arezoo Kobarfard, Farzad Keyhanfar, Fariborz Mohebbi, Shohreh Noubarani, Maryam Iran J Pharm Res Original Article In previous studies, mebudipine, a dihydropyridine calcium channel blocker, showed a considerable potential to be used in cardiovascular diseases. The aim of the current study was to develop a valid method using reversed-phase high performance liquid chromatography coupled with electrospray ionization mass spectrometry to assay mebudipine in the human plasma. Separation was achieved on a Zorbax Eclipse(®) C18 analytical column using a mobile phase consisted of methanol/water (90:10, v/v). The flow rate was 0.6 mL/min and carbamazepine was used as an internal standard (IS). This method involved the use of [M +Na](+) ions of mebudipine and IS at m/z 411 and 259, respectively with the selected ion monitoring (SIM) mode. There were no interfering peaks from endogenous components in blank plasma chromatograms. Standard curves were linear (r(2)>0.99) between 5 to 100 ng/mL. The mean extraction efficiency was about 84% and the limit of quantification for mebudipine was 5 ng/mL in plasma. The coefficient of variation and error at all of the intra-day and inter-day assessments were less than 11%. The results indicated that this method is a fast, accurate, sensitive, selective and reliable method for the determination of mebudipine in the human plasma. The assay method has been successfully used to estimate plasma concentration of mebudipine after the oral administration of 2.5 mg tablet in healthy adults. Shaheed Beheshti University of Medical Sciences 2015 /pmc/articles/PMC4518102/ /pubmed/26330862 Text en © 2015 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Asgari, Arezoo Kobarfard, Farzad Keyhanfar, Fariborz Mohebbi, Shohreh Noubarani, Maryam Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry |
title | Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry |
title_full | Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry |
title_fullStr | Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry |
title_full_unstemmed | Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry |
title_short | Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry |
title_sort | determination of mebudipine in human plasma by liquid chromatography–tandem mass spectrometry |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4518102/ https://www.ncbi.nlm.nih.gov/pubmed/26330862 |
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