Cargando…

Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry

In previous studies, mebudipine, a dihydropyridine calcium channel blocker, showed a considerable potential to be used in cardiovascular diseases. The aim of the current study was to develop a valid method using reversed-phase high performance liquid chromatography coupled with electrospray ionizati...

Descripción completa

Detalles Bibliográficos
Autores principales: Asgari, Arezoo, Kobarfard, Farzad, Keyhanfar, Fariborz, Mohebbi, Shohreh, Noubarani, Maryam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shaheed Beheshti University of Medical Sciences 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4518102/
https://www.ncbi.nlm.nih.gov/pubmed/26330862
_version_ 1782383282026971136
author Asgari, Arezoo
Kobarfard, Farzad
Keyhanfar, Fariborz
Mohebbi, Shohreh
Noubarani, Maryam
author_facet Asgari, Arezoo
Kobarfard, Farzad
Keyhanfar, Fariborz
Mohebbi, Shohreh
Noubarani, Maryam
author_sort Asgari, Arezoo
collection PubMed
description In previous studies, mebudipine, a dihydropyridine calcium channel blocker, showed a considerable potential to be used in cardiovascular diseases. The aim of the current study was to develop a valid method using reversed-phase high performance liquid chromatography coupled with electrospray ionization mass spectrometry to assay mebudipine in the human plasma. Separation was achieved on a Zorbax Eclipse(®) C18 analytical column using a mobile phase consisted of methanol/water (90:10, v/v). The flow rate was 0.6 mL/min and carbamazepine was used as an internal standard (IS). This method involved the use of [M +Na](+) ions of mebudipine and IS at m/z 411 and 259, respectively with the selected ion monitoring (SIM) mode. There were no interfering peaks from endogenous components in blank plasma chromatograms. Standard curves were linear (r(2)>0.99) between 5 to 100 ng/mL. The mean extraction efficiency was about 84% and the limit of quantification for mebudipine was 5 ng/mL in plasma. The coefficient of variation and error at all of the intra-day and inter-day assessments were less than 11%. The results indicated that this method is a fast, accurate, sensitive, selective and reliable method for the determination of mebudipine in the human plasma. The assay method has been successfully used to estimate plasma concentration of mebudipine after the oral administration of 2.5 mg tablet in healthy adults.
format Online
Article
Text
id pubmed-4518102
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Shaheed Beheshti University of Medical Sciences
record_format MEDLINE/PubMed
spelling pubmed-45181022015-09-01 Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry Asgari, Arezoo Kobarfard, Farzad Keyhanfar, Fariborz Mohebbi, Shohreh Noubarani, Maryam Iran J Pharm Res Original Article In previous studies, mebudipine, a dihydropyridine calcium channel blocker, showed a considerable potential to be used in cardiovascular diseases. The aim of the current study was to develop a valid method using reversed-phase high performance liquid chromatography coupled with electrospray ionization mass spectrometry to assay mebudipine in the human plasma. Separation was achieved on a Zorbax Eclipse(®) C18 analytical column using a mobile phase consisted of methanol/water (90:10, v/v). The flow rate was 0.6 mL/min and carbamazepine was used as an internal standard (IS). This method involved the use of [M +Na](+) ions of mebudipine and IS at m/z 411 and 259, respectively with the selected ion monitoring (SIM) mode. There were no interfering peaks from endogenous components in blank plasma chromatograms. Standard curves were linear (r(2)>0.99) between 5 to 100 ng/mL. The mean extraction efficiency was about 84% and the limit of quantification for mebudipine was 5 ng/mL in plasma. The coefficient of variation and error at all of the intra-day and inter-day assessments were less than 11%. The results indicated that this method is a fast, accurate, sensitive, selective and reliable method for the determination of mebudipine in the human plasma. The assay method has been successfully used to estimate plasma concentration of mebudipine after the oral administration of 2.5 mg tablet in healthy adults. Shaheed Beheshti University of Medical Sciences 2015 /pmc/articles/PMC4518102/ /pubmed/26330862 Text en © 2015 by School of Pharmacy, Shaheed Beheshti University of Medical Sciences and Health Services This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Asgari, Arezoo
Kobarfard, Farzad
Keyhanfar, Fariborz
Mohebbi, Shohreh
Noubarani, Maryam
Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry
title Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry
title_full Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry
title_fullStr Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry
title_full_unstemmed Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry
title_short Determination of Mebudipine in Human Plasma by Liquid Chromatography–tandem Mass Spectrometry
title_sort determination of mebudipine in human plasma by liquid chromatography–tandem mass spectrometry
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4518102/
https://www.ncbi.nlm.nih.gov/pubmed/26330862
work_keys_str_mv AT asgariarezoo determinationofmebudipineinhumanplasmabyliquidchromatographytandemmassspectrometry
AT kobarfardfarzad determinationofmebudipineinhumanplasmabyliquidchromatographytandemmassspectrometry
AT keyhanfarfariborz determinationofmebudipineinhumanplasmabyliquidchromatographytandemmassspectrometry
AT mohebbishohreh determinationofmebudipineinhumanplasmabyliquidchromatographytandemmassspectrometry
AT noubaranimaryam determinationofmebudipineinhumanplasmabyliquidchromatographytandemmassspectrometry