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RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model
Mitochondrial respiratory supercomplexes (mtRSCs) are stoichiometric assemblies of electron transport chain (ETC) complexes in the inner mitochondrial membrane. They are hypothesized to regulate electron flow, the generation of reactive oxygen species (ROS) and to stabilize ETC complexes. Using the...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4518240/ https://www.ncbi.nlm.nih.gov/pubmed/26220011 http://dx.doi.org/10.1038/srep12697 |
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author | Fischer, Fabian Filippis, Christodoulos Osiewacz, Heinz D. |
author_facet | Fischer, Fabian Filippis, Christodoulos Osiewacz, Heinz D. |
author_sort | Fischer, Fabian |
collection | PubMed |
description | Mitochondrial respiratory supercomplexes (mtRSCs) are stoichiometric assemblies of electron transport chain (ETC) complexes in the inner mitochondrial membrane. They are hypothesized to regulate electron flow, the generation of reactive oxygen species (ROS) and to stabilize ETC complexes. Using the fungal ageing model Podospora anserina, we investigated the impact of homologues of the Saccharomyces cerevisiae respiratory supercomplex factors 1 and 2 (termed PaRCF1 and PaRCF2) on mtRSC formation, fitness and lifespan. Whereas PaRCF2’s role seems negligible, ablation of PaRCF1 alters size of monomeric complex IV, reduces the abundance of complex IV-containing supercomplexes, negatively affects vital functions and shortens lifespan. PaRcf1 overexpression slightly prolongs lifespan, though without appreciably influencing ETC organization. Overall, our results identify PaRCF1 as necessary yet not sufficient for mtRSC formation and demonstrate that PaRCF1-dependent stability of complex IV and associated supercomplexes is highly relevant for maintenance of the healthy lifespan in a eukaryotic model organism. |
format | Online Article Text |
id | pubmed-4518240 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-45182402015-08-06 RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model Fischer, Fabian Filippis, Christodoulos Osiewacz, Heinz D. Sci Rep Article Mitochondrial respiratory supercomplexes (mtRSCs) are stoichiometric assemblies of electron transport chain (ETC) complexes in the inner mitochondrial membrane. They are hypothesized to regulate electron flow, the generation of reactive oxygen species (ROS) and to stabilize ETC complexes. Using the fungal ageing model Podospora anserina, we investigated the impact of homologues of the Saccharomyces cerevisiae respiratory supercomplex factors 1 and 2 (termed PaRCF1 and PaRCF2) on mtRSC formation, fitness and lifespan. Whereas PaRCF2’s role seems negligible, ablation of PaRCF1 alters size of monomeric complex IV, reduces the abundance of complex IV-containing supercomplexes, negatively affects vital functions and shortens lifespan. PaRcf1 overexpression slightly prolongs lifespan, though without appreciably influencing ETC organization. Overall, our results identify PaRCF1 as necessary yet not sufficient for mtRSC formation and demonstrate that PaRCF1-dependent stability of complex IV and associated supercomplexes is highly relevant for maintenance of the healthy lifespan in a eukaryotic model organism. Nature Publishing Group 2015-07-29 /pmc/articles/PMC4518240/ /pubmed/26220011 http://dx.doi.org/10.1038/srep12697 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Fischer, Fabian Filippis, Christodoulos Osiewacz, Heinz D. RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model |
title | RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model |
title_full | RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model |
title_fullStr | RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model |
title_full_unstemmed | RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model |
title_short | RCF1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model |
title_sort | rcf1-dependent respiratory supercomplexes are integral for lifespan-maintenance in a fungal ageing model |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4518240/ https://www.ncbi.nlm.nih.gov/pubmed/26220011 http://dx.doi.org/10.1038/srep12697 |
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