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In vitro and in vivo host range of Anopheles gambiae densovirus (AgDNV)
AgDNV is a powerful gene transduction tool and potential biological control agent for Anopheles mosquitoes. Using a GFP reporter virus system, we investigated AgDNV host range specificity in four arthropod cell lines (derived from An. gambiae, Aedes albopictus and Drosophila melanogaster) and six mo...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4518260/ https://www.ncbi.nlm.nih.gov/pubmed/26220140 http://dx.doi.org/10.1038/srep12701 |
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author | Suzuki, Yasutsugu Barik, Tapan K. Johnson, Rebecca M. Rasgon, Jason L. |
author_facet | Suzuki, Yasutsugu Barik, Tapan K. Johnson, Rebecca M. Rasgon, Jason L. |
author_sort | Suzuki, Yasutsugu |
collection | PubMed |
description | AgDNV is a powerful gene transduction tool and potential biological control agent for Anopheles mosquitoes. Using a GFP reporter virus system, we investigated AgDNV host range specificity in four arthropod cell lines (derived from An. gambiae, Aedes albopictus and Drosophila melanogaster) and six mosquito species from 3 genera (An. gambiae, An. arabiensis, An. stephensi, Ae. albopictus, Ae. aegypti and Culex tarsalis). In vitro, efficient viral invasion, replication and GFP expression was only observed in MOS55 An. gambiae cells. In vivo, high levels of GFP were observed in An. gambiae mosquitoes. Intermediate levels of GFP were observed in the closely related species An. arabiensis. Low levels of GFP were observed in An. stephensi, Ae. albopictus, Ae. aegypti and Cx. tarsalis. These results suggest that AgDNV is a specific gene transduction tool for members of the An. gambiae species complex, and could be potentially developed into a biocontrol agent with minimal off-target effects. |
format | Online Article Text |
id | pubmed-4518260 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-45182602015-08-06 In vitro and in vivo host range of Anopheles gambiae densovirus (AgDNV) Suzuki, Yasutsugu Barik, Tapan K. Johnson, Rebecca M. Rasgon, Jason L. Sci Rep Article AgDNV is a powerful gene transduction tool and potential biological control agent for Anopheles mosquitoes. Using a GFP reporter virus system, we investigated AgDNV host range specificity in four arthropod cell lines (derived from An. gambiae, Aedes albopictus and Drosophila melanogaster) and six mosquito species from 3 genera (An. gambiae, An. arabiensis, An. stephensi, Ae. albopictus, Ae. aegypti and Culex tarsalis). In vitro, efficient viral invasion, replication and GFP expression was only observed in MOS55 An. gambiae cells. In vivo, high levels of GFP were observed in An. gambiae mosquitoes. Intermediate levels of GFP were observed in the closely related species An. arabiensis. Low levels of GFP were observed in An. stephensi, Ae. albopictus, Ae. aegypti and Cx. tarsalis. These results suggest that AgDNV is a specific gene transduction tool for members of the An. gambiae species complex, and could be potentially developed into a biocontrol agent with minimal off-target effects. Nature Publishing Group 2015-07-29 /pmc/articles/PMC4518260/ /pubmed/26220140 http://dx.doi.org/10.1038/srep12701 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Suzuki, Yasutsugu Barik, Tapan K. Johnson, Rebecca M. Rasgon, Jason L. In vitro and in vivo host range of Anopheles gambiae densovirus (AgDNV) |
title | In vitro and in vivo host range of Anopheles gambiae densovirus (AgDNV) |
title_full | In vitro and in vivo host range of Anopheles gambiae densovirus (AgDNV) |
title_fullStr | In vitro and in vivo host range of Anopheles gambiae densovirus (AgDNV) |
title_full_unstemmed | In vitro and in vivo host range of Anopheles gambiae densovirus (AgDNV) |
title_short | In vitro and in vivo host range of Anopheles gambiae densovirus (AgDNV) |
title_sort | in vitro and in vivo host range of anopheles gambiae densovirus (agdnv) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4518260/ https://www.ncbi.nlm.nih.gov/pubmed/26220140 http://dx.doi.org/10.1038/srep12701 |
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