Toxic effects of Mn(2)O(3) nanoparticles on rat testis and sex hormone

BACKGROUND AND OBJECTIVE: The safety of Mn(2)O(3) nanoparticles (which are extensively used in industries) on male reproductive system is not known. Hence, we investigated the effects of Mn(2)O(3) nanoparticles on male reproductive system. MATERIALS AND METHODS: A total of 40 Wistar adult male rats were randomly assigned to four groups of 10 rats each. Three groups received Mn(2)O(3) solution in concentrations of 100, 200, and 400 ppm orally for 14 days; the control group received equal volume of saline solution. Blood samples and testicles were collected for analysis. RESULTS: Significant reduction in luteinizing hormone (LH), follicle-stimulating hormone (FSH), testosterone, spermatogonial cells, primary spermatocyte, spermatid and Leydig cell was observed in the Mn(2)O(3) nanoparticles treated groups compared with controls. CONCLUSION: Mn(2)O(3) nanoparticles significantly reduce FSH, LH, and testosterone levels resulting in a significant reduction in testicular cytology.